EMAGE mouse gene expression spatial database

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EMAGE:11619

Pigr polymeric immunoglobulin receptor ( MGI:103080)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006515. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006515_01	euxassay_006515_02	euxassay_006515_03	euxassay_006515_04

euxassay_006515_05	euxassay_006515_06	euxassay_006515_07	euxassay_006515_08	euxassay_006515_09

euxassay_006515_10	euxassay_006515_11	euxassay_006515_12	euxassay_006515_13	euxassay_006515_14

euxassay_006515_15	euxassay_006515_16	euxassay_006515_17	euxassay_006515_18	euxassay_006515_19

euxassay_006515_20	euxassay_006515_21	euxassay_006515_22	euxassay_006515_23	euxassay_006515_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11619_wholemount_strong.wlz

11619_wholemount_moderate.wlz

11619_wholemount_weak.wlz

11619_wholemount_possible.wlz

11619_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11619_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6666
Entity Detected:	Pigr, polymeric immunoglobulin receptor ( MGI:103080)
Sequence:	sense strand is shown >T6666 CTGGCTTGCAGGCTGTGCCCGAAACTGGATCACCTGGAGAGAAGGAAGTAGCTAAAACATTCTCATACAA GAAGCCAACCTGAGCGGCACAGCCCCCCTGGAAGCCACAAGCAATGAGGCTCTACTTGTTCACGCTCTTG GTAACTGTCTTTTCAGGGGTCTCCACAAAAAGCCCCATATTTGGTCCCCAGGAGGTGAGTAGTATAGAAG GCGACTCTGTTTCCATCACGTGCTACTACCCAGACACCTCTGTCAACCGGCACACCCGGAAATACTGGTG CCGACAAGGAGCCAGCGGCATGTGCACAACGCTCATCTCTTCAAATGGCTACCTCTCCAAGGAGTATTCA GGCAGAGCCAACCTCATCAACTTCCCAGAGAACAACACATTTGTGATTAACATTGAGCAGCTCACCCAGG ACGACACTGGGAGCTACAAGTGTGGCCTGGGTACCAGTAACCGAGGCCTGTCCTTCGATGTCAGCCTGGA GGTCAGCCAGGTTCCTGAGTTGCCGAGTGACACCCACGTCTACACAAAGGACATAGGCAGAAATGTGACC ATTGAATGCCCTTTCAAAAGGGAGAATGCTCCCAGCAAGAAATCCCTGTGTAAGAAGACAAACCAGTCCT GCGAACTTGTCATTGACTCTACTGAGAAGGTGAACCCCAGCTATATAGGCAGAGCAAAACTTTTTATGAA AGGGACCGACCTAACTGTATTCTATGTCAACATTAGTCACCTAACGCACAATGATGCTGGGCTGTACATC TGCCAAGCTGGAGAAGGTCCTAGTGCTGATAAGAAGAATGTTGACCTCCAGGTGCTAGCGCCTGAGCCAG AGCTGCTTTATAAAGACCTGAGGTCCTCAGTGACTTTTGAATGTGACCTGGGCCGTGAGGTGGCAAACGA GGCCAAATATCTGTGCCGGATGAATAAGGAAACCTGTGATGTGATCATTAACACCCTGGGGAAGAGGGAT CCAGACTTTGAGGGCAGGATCCTGATAACCCCCAAGGATGACAATGGCCGCTTCAGTGTGTTGATCACAG GCCTGAGGAAGGAGGATGCAGGGCACTACCAGTGTGGAGCCCACAGTTCTGGTTTGCCTCAAGAAGGCTG GCCCATCCAGACTTGGCAACTCTTTGTCAATGAAGAGTCTACCATTCCCAATCGTCGCTCTGTTGTGAAG GGAGTCACAGGAGGCTCTGTGGCCATCGCCTGTCCCTATAACCCCAAGGAAAGCAGCAGCCTCAAGTACT GGTGTCGCTGGGAAGGGGACGGAAATGGACATTGCCCCGTGCTTGTGGGGACCCAGGCCCAGGTGCAAGA AGAGTATGAAGGCCGACTGGCACTGTTTGATCAGCCAGGCAATGGTACTTACACTGTCATCCTCAACCAG CTCACCACCGAGGATGCTGGCTTCTATTGGTGTCTTACCAATGGTGACTCTCGCTGGAGAACCACAATAG AACTCCAGGTTGCCGAAGCTACAAGGGAGCCAAACCTTGAGGTGACGCCACAGAACGCAACAGCAGTACT AGGAGAGACCTTCACCGTTTCCTGCCACTATCCGTGCAAATTCTACTCCCAGGAGAAATACTGGTGCAAG TGGAGCAACAAGGGTTGCCACATCCTGCCAAGCCATGACGAAGGTGCCCGCCAATCTTCTGTGAGCTGCG ACCAGAGCAGCCAGCTGGTCTCCATGACCCTGAACCCGGTCAGTAAGGAAGATGAAGGCTGGTACTGGTG TGGGGTAAAGCAAGGCCAGACCTATGGAGAAACTACCGCCATCTATATAGCAGTTGAAGAGAGGACCAGA GGGTCATCCCATGTCAACCCAACAGATGCAAATGCACGTGCCAAAGTCGCTCTGGAAGAAGAGGTAGTGG ACTCCTCCATCAGTGAAAAAGAGAACAAAGCCATTCCAAATCCCGGGCCTTTTGCCAACGAAAGAGAGAT ACAGAATGTGGGAGACCAAGCTCAGGAGAACAGAGCATCTGGGGATGCTGGCAGTGCTGATGGACAAAGC AGGAGCTCCAGCTCCAAAGTGCTGTTCTCCACCCTGGTGCCCCTGGGTCTGGTGCTGGCAGTGGGTGCTA TAGCTGTGTGGGTGGCCAGAGTCCGACATCGGAAGAATGTAGACCGCATGTCAATCAGCAGCTACAGGAC AGACATTAGCATGGCAGACTTCAAGAACTCCAGAGATTTGGGAGGCAATGACAACATGGGGGCCTCTCCA GACACACAGCAAACAGTCATCGAAGGAAAAGATGAAATCGTGACTACCACGGAGTGCACCGCTGAGCCAG AAGAATCCAAGAAAGCAAAAAGGTCATCCAAGGAGGAAGCTGACATGGCCTACTCGGCATTCCTGCTTCA GTCCAGCACCATAGCTGCACAGGTCCACGATGGTCCCCAGGAAGCCTAGGCAGTGCTGACCACCCACCCT TGCCTGTGACAATCAACTTGAGAATCACACTGATCCGCTCGCAGCCCACACTCACCCATCACCTCCGCTC TTCCCTCCTGTCCTCAGAGGTGTGCTGGTTCCTTCCTCGGCCATGGAAGCCTGGCCTAGTTACGCCTGTT TAGGAGAGAGTGTGAGGCGTTCTTTTCTCTATGAAGAGAGTGAGGTGGAAATGAGGAGGAGGTGAACCTG AGAGACATCTCTGGAGGAAGAGGGTTCACAATAGGGGCTCGTTTCAGGAGAAAAGGCCATTTGAATCTTC TTTATAACCATATGATAGGATGTCAGCGTAACTCTTCTCTCCTCCATCTCTCCTTTCCTATCCTCTTGAT TCAAACAACACATCTGAGAACTCACTAGGCTTCAGTGCCTACTAAATGCTGAGAGCCAGGCCACAATCTT TCTATAAATATTACTGGAAGAGATGCCATCTCCTCCCAGATTCTGTCTTTTCATTAAGATAAGACATCAT TACCAGGCATACCTCCTGCCTCTGTGCCTCATAGGCATACACAAGCCATAAGGGCATCATGATTTTCAGA TGAGAAGAGATGTTTCTCAAGAGTGCCTAGTGAGATAGACTAGCGTCAAACCAGATGTGGCAACTCCTGG CTCTTGGCCTACGATCTGTCTTCAAGACCTCTGCTCTTATTAGAGGGAGAACTTTAGCATGGGGGAAAGT AAGAGAAAATGAGTTAAAGGAGCATGGTGGTGTGCTCCTGCAATCCCAATATTAAGAGGTAAAAATAGGA TCAGAAGTTCAAGGTAATCCTTGGCTACCTAGGGAGTTTAAGGCCAGCCTGGGATACATTGAGACTTGGA TTCAAAGAAGCAGGAGGAGGGAGCAGGAAGAGGAGGAGTAGAAAGGGGGAAGAAGGAGAAGGAAAAAAGA AGAAGAGGAGGAAGAGGAGGAGGAGGAGGAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGATGAAGAAGAAGAAGAAGA AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601095 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601095 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11621 same embryo
	EMAGE:11620 same embryo
	EMAGE:11618 same embryo
	EurExpress:euxassay_006515 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source