EMAGE mouse gene expression spatial database

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EMAGE:11633

Ppargc1a peroxisome proliferative activated receptor, gamma, coactivator 1 alpha ( MGI:1342774)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006699. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006699_01	euxassay_006699_02	euxassay_006699_03	euxassay_006699_04

euxassay_006699_05	euxassay_006699_06	euxassay_006699_07	euxassay_006699_08	euxassay_006699_09

euxassay_006699_10	euxassay_006699_11	euxassay_006699_12	euxassay_006699_13	euxassay_006699_14

euxassay_006699_15	euxassay_006699_16	euxassay_006699_17	euxassay_006699_18	euxassay_006699_19

euxassay_006699_20	euxassay_006699_21	euxassay_006699_22	euxassay_006699_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11633_wholemount_strong.wlz

11633_wholemount_moderate.wlz

11633_wholemount_weak.wlz

11633_wholemount_possible.wlz

11633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 04 05 weak expression: see section 22 23

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 22 23

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 22 23

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 22 23

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 05 06 weak expression: see section 22 23

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 22 23

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 05 06 19 moderate expression: see section 02 03 07 08 09 20 21 22 weak expression: see section 01 10 11 16 17 18

telencephalon ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 18 19 20 21 22

pons mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 11 12 13 14 15 16 17 18

midbrain mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 18 19 20 21 weak expression: see section 07 08 17

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

single cell

moderate expression: see section 06 07 08 10 11 15 16 17

anterior naris

weak

regional

weak expression: see section 16 18

external naris

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

posterior naris

moderate

regional

moderate expression: see section 14

heart left ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11

heart right ventricle

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

lower jaw incisor

strong

regional

strong expression: see section 12 13 16 17

oral epithelium

moderate

regional

moderate expression: see section 08 09 16 17 18 weak expression: see section 10

upper jaw incisor

strong

regional

strong expression: see section 13 17

bladder

moderate

regional

Expression in the fundus region.

kidney calyx

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20

urethra of male

moderate

regional

moderate expression: see section 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8100
Entity Detected:	Ppargc1a, peroxisome proliferative activated receptor, gamma, coactivator 1 alpha ( MGI:1342774)
Sequence:	sense strand is shown >T8100 TGAAAAAGCTTGACTGGCGTCATTCGGGAGCTGGATGGCTTGGGACATGTGCAGCCAAGACTCTGTATGG AGTGACATAGAGTGTGCTGCTCTGGTTGGTGAGGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGACC TTTCTGAACTTGATGTGAATGACTTGGATACAGACAGCTTTCTGGGTGGATTGAAGTGGTGTAGCGACCA ATCGGAAATCATATCCAACCAGTACAACAATGAGCCTGCGAACATATTTGAGAAGATAGATGAAGAGAAT GAGGCAAACTTGCTAGCGGTCCTCACAGAGACACTGGACAGTCTCCCCGTGGATGAAGACGGATTGCCCT CATTTGATGCACTGACAGATGGAGCCGTGACCACTGACAACGAGGCCAGTCCTTCCTCCATGCCTGACGG CACCCCTCCCCCTCAGGAGGCAGAAGAGCCGTCTCTACTTAAGAAGCTCTTACTGGCACCAGCCAACACT CAGCTCAGCTACAATGAATGCAGCGGTCTTAGCACTCAGAACCATGCAGCAAACCACACCCACAGGATCA GAACAAACCCTGCCATTGTTAAGACCGAGAATTCATGGAGCAATAAAGCGAAGAGCATTTGTCAACAGCA AAAGCCACAAAGACGTCCCTGCTCAGAGCTTCTCAAGTATCTGACCACAAACGATGACCCTCCTCACACC AAACCCACAGAAAACAGGAACAGCAGCAGAGACAAATGTGCTTCCAAAAAGAAGTCCCATACACAACCGC AGTCGCAACATGCTCAAGCCAAACCAACAACTTTATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCC CAAGGGTTCCCCATTTGAGAACAAGACTATTGAGCGAACCTTAAGTGTGGAACTCTCTGGAACTGCAGGC CTAACTCCTCCCACAACTCCTCCTCATAAAGCCAACCAAGATAACCCTTTCAAGGCTTCGCCAAAGCTGA AGCCCTCTTGCAAGACCGTGGTGCCACCGCCAACCAAGAGGGCCCGGTACAGCGAGTGTTCTGGTACCCA AGGCAGCCACTCCACCAAGAAAGGGCCCGAGCAATCTGAGTTGTACGCACAACTCAGCAAGTCCTCAGGG CTCAGCCGAGGACACGAGGAAAGGAAGACTAAACGGCCCAGTCTTCGGCTGTTTGGTGACCATGACTATT GTCAGTCACTCAATTCCAAAACGGATATACTCATTAACATATCACAGGAGCTCCAAGACTCTAGACAACT AGACTTCAAAGATGCCTCCTGTGACTGGCAGGGGCACATCTGTTCTTCCACAGATTCAGGCCAGTGCTAC CTGAGAGAGACTTTGGAGGCCAGCAAGCAGGTCTCTCCTTGCAGCACCAGAAAACAGCTCCAAGACCAGG AAATCCGAGCGGAGCTGAACAAGCACTTCGGTCATCCCTGTCAAGCTGTGTTTGACGACAAATCAGACAA GACCAGTGAACTAAGGGATGGCGACTTCAGTAATGAACAATTCTCCAAACTACCTGTGTTTATAAATTCA GGACTAGCCATGGATGGCCTATTTGATGACAGTGAAGATGAAAGTGATAAACTGAGCTACCCTTGGGATG GCACGCAGCCCTATTCATTGTTCGATGTGTCGCCTTCTTGCTCTTCCTTTAACTCTCCGTGTCGAGACTC AGTGTCACCACCGAAATCCTTATTTTCTCAAAGACCCCAAAGGATGCGCTCTCGTTCAAGATCCTTTTCT CGACACAGGTCGTGTTCCCGATCACCATATTCCAGGTCAAGATTAAGGTCCCCAGGCAGTAGATCCTCTT CAAGATCCTGTTACTACTATGAATCAAGCCACTACAGACACCGCACACACCGCAATTCTCCCTTGTATGT GAGATCACGTTCAAGGTCACCCTACAGCTGTAGGCCCAGGTACGACAGCTATGAAGCCTATGAGCACGAA AGGCTCAAGAGGGATGAATACCGCAAAGAGCACGAGAAGCGGGAGTCTGAAAGGGCCAAGCAGAGAGAGA GGCAGAAGCAGAAAGCAATTGAAGAGCGCCGTGTGATTTACGTTGGTAAAATCAGACCTGACACAACGCG GACAGAATTGAGAGACCGCTTTGAAGTTTTTGGTGAAATTGAGGAATGCACCGTAAATCTGCGGGATGAT GGAGACAGCTATGGTTTCATCACCTACCGTTACACCTGTGACGCTTTCGCTGCTCTTGAGAATGGATATA CTTTACGCAGGTCGAACGAAACTGACTTCGAGCTGTACTTTTGTGGACGGAAGCAATTTTTCAAGTCTAA CTATGCAGACCTAGATACAAACTCAGACGATTTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTG GATTTTGATAGTTTACTGAAGGAAGCTCAGAGAAGCTTGCGCAGGTAACATGTTCCCAGGCTGAGGAATG ACAGAGAGATGGTCAATACCTCATGGGACAGCGTGTCCTTTCCCAAGACTCTTGCAAGTCATACTTAGGA ATTTCTCCTACTTTACACTCTCTGTACAAAAATAAAACAAAACAAAACAACAATAACAACAACAACAACA ACAACAACAATAACAACAACAACCATACCAGAACAAGAACAACGGTTTACATGAACACAGCTGCTGAAGA GGCAAGAGACAGAATGATAATCCAGTAAGCACACGTTTATTCACGGGTGTCAGCTTTGCTTTCCCTGGAG GCTCTTGGTGACAGTGTGTGTGCGTGTGTGTGTGTGGGTGTGCGTGTGTGTATGTGTGTGTGTGTACTTG TTTAGAAAGTACATATGTACACATGTGAGGACTTGGGGGCATCTGAACAGAACGAACAAGGGCGACCCCT TCAAATGGCAGCATTTCCATGAAGACACACTTAAAACCTACAACTTCAAAATGTTCGTATTCTATACAAA AGGAAAATAAATAAATATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30094033 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30094033 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11634 same embryo
	EMAGE:11636 same embryo
	EMAGE:11637 same embryo
	EMAGE:11635 same embryo
	EMAGE:11638 same embryo
	EurExpress:euxassay_006699 same experiment
	MGI:4827349 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source