EMAGE mouse gene expression spatial database

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EMAGE:11637

Itga4 integrin alpha 4 ( MGI:96603)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006687. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006687_01	euxassay_006687_02	euxassay_006687_03	euxassay_006687_04

euxassay_006687_05	euxassay_006687_06	euxassay_006687_07	euxassay_006687_08	euxassay_006687_09

euxassay_006687_10	euxassay_006687_11	euxassay_006687_12	euxassay_006687_13	euxassay_006687_14

euxassay_006687_15	euxassay_006687_16	euxassay_006687_17	euxassay_006687_18	euxassay_006687_19

euxassay_006687_20	euxassay_006687_21	euxassay_006687_22	euxassay_006687_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11637_wholemount_strong.wlz

11637_wholemount_moderate.wlz

11637_wholemount_weak.wlz

11637_wholemount_possible.wlz

11637_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11637_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

weak

regional

weak expression: see section 09 10 11 12 13

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 05 06 07 08 09 11 12 13 14 15 16

telencephalon mantle layer

moderate

regional

moderate expression: see section 04 19 weak expression: see section 05 18

pons mantle layer

weak

regional

weak expression: see section 03 04 05 14 16

midbrain mantle layer

weak

regional

weak expression: see section 05 07 08 13 14

ventral grey horn

moderate

regional

moderate expression: see section 14 weak expression: see section 07 08 09 10 11 12 13

heart ventricle

moderate

regional

moderate expression: see section 06 07 12 13 14 weak expression: see section 08 09 10 11 15 16 17 18

bladder

weak

regional

Expression in the fundus region.

urethra of male

weak

regional

weak expression: see section 13 14 15

left lung

moderate

regional

moderate expression: see section 07 08 09 10 weak expression: see section 03 04 05 06

right lung

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8101
Entity Detected:	Itga4, integrin alpha 4 ( MGI:96603)
Sequence:	sense strand is shown >T8101 CTCCACCAAGAGCGCATGGCTGCGGAAGCGAGGTGCAGACCGAGGTCCCGAGGGATCGCCCTCCGGGAAG CGGTGATGCTGTTGTTGTACTTCGGGGTGCCAACCGGGCACTCCTACAACCTGGACCCGGAGAATGCACT GCTGTACCAGGGCCCCTCCGGCACGCTGTTTGGCTACTCGGTGGTGCTGCACAGCCACGGGTCGAAGCGC TGGCTCATCGTGGGGGCTCCCACTGCCAGCTGGCTCTCTAATGCCTCAGTGGTCAATCCTGGGGCGATTT ACAGATGCGGGATCAGAAAGAATCCAAACCAGACCTGCGAACAGCTCCAGCTGGGTAGCCCCAGTGGAGA GCCTTGTGGGAAGACATGCCTGGAGGAGAGGGATAACCAGTGGCTGGGGGTCACCCTTTCCAGACAGCCT GGAGAAAATGGCTCTATCGTGACTTGTGGGCACAGGTGGAAAAATATTTTTTACATGAAGAGCGATAACA AACTCCCCACTGGCATTTGCTACGTCATGCCTTCTGATTTGCGGACAGAACTGAGTAAAAGGATGGCCCC GTGTTACAAAGATTATACGAGAAAATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCTAGTTTT TACACACAGGATTTAATTGTGATGGGGGCCCCGGGATCATCGTACTGGACTGGCACCGTCTTTGTCTACA ATATAACTACAAACCAATACAAAGCATTTGTAGACAGACAGAACCAAGTAAAATTTGGAAGCTACTTAGG CTACTCAGTTGGAGCTGGACATTTTCGAAGTCCACATACTACCGAAGTCGTGGGAGGAGCCCCTCAACAC GAACAGATAGGAAAAGCATATATATTTAGCATTGATGAAAACGAACTGAACATCGTATATGAAATGAAAG GTAAAAAGCTTGGCTCATACTTTGGAGCTTCTGTCTGCGCTGTGGACCTCAATGCAGATGGCTTCTCAGA TCTCCTTGTTGGAGCTCCCATGCAGAGCACCATCAGGGAGGAAGGAAGAGTATTCGTGTACATCAACTCT GGCATGGGAGCTGTGATGGTTGAAATGGAAAGGGTCCTTGTCGGAAGTGACAAATATGCTGCAAGATTTG GGGAGTCTATAGCGAATCTTGGCGACATTGACAATGACGGCTTTGAAGATATTGCTATTGGTGCACCACA AGAAGACGACTTGCGAGGTGCTGTCTACATTTACAATGGCCGAGTCGATGGAATCTCCTCCACCTACTCA CAGAGAATTGAAGGACAGCAAATCAGCAAATCATTAAGGATGTTTGGACAATCTATCTCAGGACAAATTG ATGCAGACAACAATGGATATGTTGATGTAGCCATTGGTGCATTTCACTCTGATTCTGCAGTGTTGCTAAG GACAAGGCCTGTAGTGATTGTTGAAGCATCTTTAAGCCATCCTGAGTCTGTAAATAGGACAAAGTTTGAC TGTACTGAAAATGGACTTCCATCTGTGTGCATGCATCTTACACTGTGTTTCTCATATAAAGGCAAAGAGG TCCCAGGCTACATCGTTTTGTTTTACAATGTGAGCTTGGATGTGCACAGGAAGGCAGAGTCTCCGTCAAG ATTTTATTTCTTCTCTAATGGGACTTCTGACGTGATTACAGGAAGCATACGAGTTTCAAGCAGTGGAGAG AAATGTAGGACACACCAGGCATTCATGCGGAAAGATGTGCGAGACATCCTTACCCCCATTCATGTAGAGG CCACATACCACCTTGGGCATCATGTGATCACCAAACGAAACACTGAGGAATTTCCACCACTCCAGCCGAT CCTTCAGCAGAAGAAAGAAAAAGACGTTATTAGAAAAATGATAAACTTTGCAAGGTTTTGTGCCTATGAA AATTGCTCTGCTGATCTCCAAGTTTCTGCAAAAGTTGGATTTTTGAAGCCATATGAAAATAAAACCTATC TTGCTGTTGGGAGCATGAAGACCATAATGCTAAACGTGTCCTTGTTCAACGCTGGCGATGATGCTTACGA AACCACTCTGAATGTCCAACTCCCCACAGGCCTTTATTTCATTAAGATCTTAGACCTGGAAGAGAAACAA ATAAACTGCGAAGTGACTGAGAGCTCAGGCATAGTGAAGCTTGCCTGCAGCCTAGGTTACATATATGTGG ATCGCCTCTCAAGGATAGACATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGGGCACATGAGGACCT CAGCATCAGTGTGCATGCCTCCTGTGAAAACGAGGGTGAATTGGACCAAGTGAGGGACAACAGAGTAACC TTAACGATACCTCTAAGGTATGAGGTTATGCTGACTGTTCATGGGCTTGTGAACCCAACTTCATTTGTGT ATGGATCTAGCGAAGAAAACGAGCCAGAAACATGCATGGCCGAGAAGCTGAACCTCACTTTCCATGTTAT AAACACTGGGATTAGCATGGCTCCAAATGTTAGTGTGAAAATAATGGTACCAAATTCTTTTCTCCCTCAA GATGATAAGTTGTTCAACGTTTTGGATGTCCAGACAACTACAGGGCAATGCCATTTTAAACACTATGGAA GAGAGTGTACATTTGCACAGCAAAAAGGCATAGCGGGGACGTTGACCGATATAGTCAAATTCCTATCAAA GACTGATAAGAGACTCCTGTATTGCATGAAAGCTGATCAACACTGTTTAGATTTCTTATGCAATTTCGGA AAAATGGAAAGTGGGAAGGAAGCCAGCGTTCATATTCAGCTGGAGGGCAGGCCATCCATCTTGGAAATGG ATGAGACCTCATCACTCAAGTTTGAAATAAAAGCAACAGCTTTTCCAGAGCCACACCCAAAAGTTATTGA ACTAAATAAAGATGAGAACGTGGCCCATGTTTTCTTGGAAGGGCTCCATCATCAAAGACCCAAACGACAT TTCACCATCATTATTATTACCATCAGCTTGCTACTTGGACTTATTGTACTTTTATTAATTTCATGTGTTA TGTGGAAGGCTGGATTCTTTAAAAGACAGTACAAATCTATCCTACAAGAAGAAAACAGGAGAGACAGCTG GAGTTATGTCAACAGCAAAAGCAATGATGACTGAAGACTTCTACACTGAGAGAACTGAAAAACTCAGGTT AGGAAAAAGAAATCCTGTTCAGAAGACCCGTCAGAATTTCTTCTTTTTTTCCATGTGCTTATGATTTTGT GACATACTCTTAGTGCAGGGGAAATCTTCAAGAAAGAAGCTACCCAAAGGTGGCTTGTCAGCTTCGGTGG ATGAGTGAAGCAAAACACTAAAGCTCTGGATGTACCGGAGAGGTGACCTGTTTAAGACAACTTAAAGCTA GAGAGAATCCAGACTCAGCAGGGCCGACTTAAAGGGAATGATTTTTCAACATCACTGATGAAGTGGCTCA TCTCAGTGAAATGGATGCCATGATGTGGAAACTTGTTGGCTTCAAATACTTTTATCTTCAAACTATGATC ATGATCTTTGAATTCACTGAGACTCTTTCAAATGGCTGTTCCAAGATTGTCTAATGGATAAGTCATTTTT TATTAGATATTTTCTTTATTTACATTTCAAATGTTATCCCCTTTCCTAGTTTCCACTCCAAAAATCCCCT ATCCTCTCCCCTGTCCCCTGCTCCCCAATCGACCCACTCCCGCTTCCTGGCCAAGGCGTTCCCCTATACT GGGGAATAGAACCTTCACAGGACCAAGGGCCTCTCCTCCTATTGACAGAGCACAGGGTCCCCAATGAAGG AGTTAGAGAAAATACCCAAGGAGCTGAAGGGGTTTGCAGCCCCATAGGAGGAACAACAATATGAACTAAC CAGTAACCCCAGAGCTCCTTGGGACTAAACCACCAACCAAAGAAAACACACGGAGAGACGCATGGCTCTA GCTGGATCAATCATTTTAAAGCAGAGGACCTGAAGACTGCTCTTGGTGGTGCTGCATCCCTGGGCTGTGT CCCTACAGCCCTTTTACTCTGCAGATAGGAACTGTAAAATCCTAAAGAACAAAATCCGTTTGCAGCAGGA TGGCTTCTGGGGAAATGGTCAGCTGCGGACTCTTGTGTGTATGTACATATACTTTTTTAAAGGATAAAAA TCTCAATCTACTTCTTTATTAAGAACACCCAGGAATAGTTTGCATCTTCTAAGCTTCACCTTTAATACCA ATTCTTAAGAATTTTATCCTCATAGCAGATTATAAATATCGCACTTTAGCTGATACACACTGATACAACA TGAACTGCAGCTCACTGATGATCGAAAATTGTGTGAAGAGAGATTTAGGAACCAATATTTCATAAAACAT TCACTGCATCAAATTATGCAAACATGAGTATCTGCTAGGATATTGTTGTTTCTCTTGGTCTGGTTGAATA TACATAAACTAGGACTCTAAAACACCACATTCTGTCATAGAAATGGAGACACAAAAATCATAGTTGGAAC TGCAAATCACTGATCAAGTTTGTGTGCAGTGGCATCAAAATTCCCATGCGATAATGCATGCTTGATGTTG TTGAACAAGATACTATTTTAAAATGAAATAGCTTCTACCTGGGTTTCACATAATTTGTCTGTAATTACAA TCATTTTTGTAGTATTTATTTCATATATGTAGTTTGATAAATGTAAGTTTTCATTTGAATCTGTTATGTG TAAGCAAGCTAAGGCCAGACTTTGAACATGTGCTGGGTAAAAGGGGCAGCCTTCAGTGTTGTGAAGAAAG ATCGCTTTACAGGAAGGGTTTGATGCCAGCTGGCCCTTCCTCAGATTTCCTGACTTGTGAGGATGGCTTG TTCACAAGCCTCTGGCTGGAGCCGTGGAGGGAGGTTTTGAGTGCTGAAACTTGAGCATAGAGCATCAGAT CTGGGTGAGCAGAAACCATATTTAATTAGATGCTTATATTTTTAAGGGATGCTTTTTTGTGTAAAAGGTT TAAGTTGATGGTTTTCTTATAAAAATTCAAGTAGTTATTTCATGAGGATGCCAAAGTTAATAGATGAGAA ATCAATATCATTTTAGACCTACCGTTTCAAGGAATGGTCTCTGAGGAGAGTTAAATGTGGATCATTGCAC TGAAAACCGACTCCTGCCTTTCAGGTACAAAAGAACGTAGCTACCTCCTGGGCCACATTTTGCCCAATTA GAAAGAAATATTTTTATATTTATTAATAAAACTTAGAATTTTATATAAGCTTTAAAATGTAGTTATTTCA AAAGATGCTGTTATGGGATGGGTAGTAGAATAGTAGCCTAAGAGGAAAGAATGTTTTTTAAGTGGGTGTT CGTGGTTATGTTACTATCTCACTGTGAAGTCTGAGATTTTACAGAAGTTTTGTTTTACTAGGTTACAGGA GATACTAAAGAATTATCTTCAACAGTATTGAGAGAGCACAATGTTGTTAAAATTAGATCTAACAAACACC TTCCAAAAATCAAAACTTTGATTTTTTTCATTAATTTCATATTTTTTGATTTTCAAATACCTGGCTACTA TTTTAATTATATTAAAATATTTTAATTGCATTAAAGTAGATACTTTAATTATATTAAATAGATAGCTTCA TTAAAATGTGTTGCAGTAAATCTCTCCAACCCAAATATGCCTTGGCAATGAAAACACAACACAGTTAATA TGATTGCATGCTGTGCGCCTAGATTGGGCAGATCTACTGCTACACTACTGTCTTCCACAGCTTATGAGAC CCCTTAGAACTTGCGGTTTCTCCAGGCCATGTGCTTCTGCTCTGCTTCACTTCATCTTCCTCCCCCTCTG CATCCTCTCCCTCTTCCATTTTCTCCTTCTTTTCTCTCCCTACCTTCGGCTCCGCCTTCCCTTTTATCTG CCCAATCATCAGCTCTCCTTTATTTTACAAATTAAGGTGGGAATCAGGTTTACAGGAAATCACCTGAGTG CTGACTCATTCCTTGTTCACAACCGCTCATGGGAGAACAGAATTAACATCAAATATAATTAGCCCCAGGG CTATCCTCAACATTTACCCCCTTCTGTCCAATTAAAAGACTATTTTATCTCAGATATAATTGAACATAAC AATTATTATTTTGTAATTTATAAAGTACAGGAGACCTAACACCCAGTCCATCATCTTTGTTAATTAAATA AAAATCTCTGTCATCTATCCTTACTTAAAAGACTATAGTTCTGCACCTGGCTAGTTCTGCATCTGAAAAC CATCCTCTCAAATCTATTCCTCTCAAAGTGAAAAGCCTGGGCTCGCCTGAGACTATGTAGTTTTTCAACC TCAACATAAATCCAAGAATGACTGATATTAACTGAAAATATATAGGAAGCCTAACATAGCTTCCAAAATT TAGATAATTTGTTGAGACCACTGGTCACTTGGACAGTCCCTTACTTCAACAGGGTGGAGCTTTGATCTTC AGGCTACTGGCCTTAGTCATCTGACAAGACTTAGAGAAACAAGAATATTAAGGACTAGCCTACTGTCTCG GCAGAATCAAGCTGTCCTAACCTGTAGTTGTGTCCTTTCTTTGGACAGTACTATATCTGTAGATGAAATG AGCCAATTCTTGCCTAGTGACTTTCACCACAACTAGAGTAACTCAAAGATGCTCAGTTTCTTCTTTGAAT CCAAGACAGGGAAAGCTGTCAGGAGCAGACTAGTCTCAACAACAAGTGAATAAATAACATCAAACTTCAC ATTCTGTGGACTTCTGATGTTTTTGGAAAACCAATTATCTATATGAAGTAATCTGAACTGTTCTCTGAAC CTCTCTCAGCCATTTCTGATTAAAATAACTGAAAACACCCTAACAATAAACTCAGAGCCAAGAATTTCCT ATCTGACCCTTAACTCACAGGCTTAAACATCTCAGCTCTGTTTATCATAACAGCAATTGAAGGACTGGGT CTAAGCCTTGTACTTCAAAATTTTTAGTATCAAAGACAACCTATAATAACCACCCGCAGCCGCAAAGCTT AGGGAACTGGGATGATGAATCTTCATAACTTCTTCAAGCTGATATGGGCGTTGAGATATTTTTGAAGGAA GGGGGAAGAATAGGGAAAGGGGGAGTTAGTTGGCCTTAGGAAGGCCCCACCATGATTGTATTAGTTTCTG ATGTCTCTGTCCTGACTGGATCCGGATGAAAAACCAAGATATCAGTTCAGGCAAGTCAGTTCAGCATGTC TGATGATGAAATTCACCAAGGCTGTATATTCTGTAATATATAAATCTCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30093248 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30093248 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11634 same embryo
	EMAGE:11636 same embryo
	EMAGE:11635 same embryo
	EMAGE:11638 same embryo
	EMAGE:11633 same embryo
	EurExpress:euxassay_006687 same experiment
	MGI:4825642 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source