EMAGE mouse gene expression spatial database

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EMAGE:11667

Prim1 DNA primase, p49 subunit ( MGI:97757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006997_01	euxassay_006997_02	euxassay_006997_03	euxassay_006997_04

euxassay_006997_05	euxassay_006997_06	euxassay_006997_07	euxassay_006997_08	euxassay_006997_09

euxassay_006997_10	euxassay_006997_11	euxassay_006997_12	euxassay_006997_13	euxassay_006997_14

euxassay_006997_15	euxassay_006997_16	euxassay_006997_17	euxassay_006997_18	euxassay_006997_19

euxassay_006997_20	euxassay_006997_21	euxassay_006997_22	euxassay_006997_23	euxassay_006997_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11667_wholemount_strong.wlz

11667_wholemount_moderate.wlz

11667_wholemount_weak.wlz

11667_wholemount_possible.wlz

11667_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11667_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9999
Entity Detected:	Prim1, DNA primase, p49 subunit ( MGI:97757)
Sequence:	sense strand is shown >T9999 GCGCAGGGATGGAGGCCTGTCTTGTTCCCGCCAATTCTCGTGTCAGTCCTGTCCGGGTCTAGTTCTTTAC TTCATGGAGCCATTTGATCCTGCGGAGCTGCCGGAGCTACTCAAGCTTTATTACCGAAGGCTCTTTCCCT ACGCCCAGTACTATCGCTGGCTCAACTATGGCGGAGTGACAAAGAATTACTTTCAACACCGTGAATTTTC ATTCACACTGAAAGATGATATTTACATTCGCTACCAGTCCTTCAACAACCAGAGTGAACTGGAGAAGGAG ATGCAGAAAATGAATCCATATAAGATTGATATAGGCGCAGTATATTCTCACAGACCCAATCAACACAATA CAGTGAAGCTGGGAGCTTTCCAGGCCCAGGAGAAAGAACTGGTCTTTGACATCGACATGACAGACTACGA TGACGTAAGGAGATGCTGCAGTTCTGCAGACATATGTTCCAAATGCTGGACTCTCATGACAATGGCCATG CGCATCATAGACCGGGCATTGAAGGAGGACTTTGGATTTAAGCACCGCCTCTGGGTCTATTCAGGAAGGA GAGGTGTGCACTGTTGGGTCTGTGATGAATCCGTTCGAAAGCTGTCCTCTGCTGTGCGCTCCGGGATCGT GGAGTACTTGAGTCTTGTAAAGGGTGGTCAAGATGTTAAAAAGAAAGTTCACCTAAATGAGAAAGTTCAC CCTTTTGTCAGAAAATCTATAAACATAATTAAAAAGTACTTTGAGGAATATGCCTTAGTCGGCCAAGATA TTCTTGAAAATAAAGAAAACTGGGATAAGATTTTAGCCCTTGTTCCTGAGACAATTCATGATGAGCTGCA AAGAGGGTTCCAGAAGTTCCACAGTTCACCTCAGCGCTGGGAACACCTGAGGAAAGTGGCCAACTCATCT CAGAATATGAAGAACGATAAGTGCGGCCCCTGGCTGGAGTGGGAGGTCATGCTCCAGTACTGTTTCCCAC GCCTGGATGTCAACGTCAGCAAGGGAGTCAATCACTTACTGAAGAGCCCTTTCAGTGTTCATCCCAAGAC AGGTCGGATTTCTGTGCCTATCGATTTTCATAAAGTGGATCAATTTGATCCGTTTACTGTCCCAACTATA AGTGCCATCTGCCGGGAATTGGATATGGTTTCCACTCATGAGAAGGAAAAGGAGGAGAATGAAGCTGACA GCAAACACAGAGTCAGAGGTTACAAGAAGACCAGTCTAGCACCGTATGTGAAAGTATTTGAACAGTTTCT TGAAAACCTGGATAAATCCCGGAAAGGGGAACTTCTCAAGAAGAGCGATTTACAAAAAGATTTCTGAAGA AAACAGCTCCCATCAAACCAACGTGAATGCCTTCTACCTTCATCCATGGAGCATTTAATAAATACAGTAG AATCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30504228 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30504228 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11666 same embryo
	EMAGE:11668 same embryo
	EMAGE:11669 same embryo
	EMAGE:11665 same embryo
	EMAGE:11670 same embryo
	EurExpress:euxassay_006997 same experiment
	MGI:4827412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source