EMAGE mouse gene expression spatial database

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EMAGE:11703

Nr1h2 nuclear receptor subfamily 1, group H, member 2 ( MGI:1352463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006874. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006874_01	euxassay_006874_02	euxassay_006874_03	euxassay_006874_04

euxassay_006874_05	euxassay_006874_06	euxassay_006874_07	euxassay_006874_08	euxassay_006874_09

euxassay_006874_10	euxassay_006874_11	euxassay_006874_12	euxassay_006874_13	euxassay_006874_14

euxassay_006874_15	euxassay_006874_16	euxassay_006874_17	euxassay_006874_18	euxassay_006874_19

euxassay_006874_20	euxassay_006874_21	euxassay_006874_22	euxassay_006874_23	euxassay_006874_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11703_wholemount_strong.wlz

11703_wholemount_moderate.wlz

11703_wholemount_weak.wlz

11703_wholemount_possible.wlz

11703_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11703_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21 22

vibrissa

moderate

regional

moderate expression: see section 08 09 10 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9194
Entity Detected:	Nr1h2, nuclear receptor subfamily 1, group H, member 2 ( MGI:1352463)
Sequence:	sense strand is shown >T9194 GGCGAAGTTACTTTTGCTTTTCGCTCAGCAAGCGCTGTTGCTTCGAGCTACTCCCAGGCTTCTGAAGTTA CTTCCAAAGTGCTGTGGAGGCACAATCACCGGTGCGGACACAGAGCTCTCGCCTCCCACGGCCGTTTCCA GGGCAACAGAGTCGGAGACCCCCTGCGACCCCCCTCCCGATCGCCGGTGCAGTCATGAGCCCCGCCTCCC CCTGGTGCACGGAGAGGGGCGGGGCCTGGAACAAGGCTGCTTCGTGACCCACTATGTCTTCCCCCACAAG TTCTCTGGACACTCCCGTGCCTGGGAATGGTTCTCCTCAGCCCAGTACCTCCGCCACGTCACCCACTATT AAGGAAGAGGGGCAGGAGACTGATCCTCCTCCAGGCTCTGAAGGGTCCAGCTCTGCCTACATCGTGGTCA TCTTAGAGCCAGAGGATGAGCCTGAGCGCAAGCGGAAGAAGGGGCCGGCCCCGAAGATGCTGGGCCATGA GCTGTGCCGCGTGTGCGGAGACAAGGCTTCGGGCTTCCACTACAACGTGCTCAGCTGTGAAGGCTGCAAA GGCTTCTTCCGGCGCAGTGTGGTCCACGGTGGGGCCGGGCGCTATGCCTGTCGGGGCAGCGGAACCTGCC AGATGGATGCCTTCATGCGGCGCAAGTGCCAGCTCTGCCGGCTGCGCAAGTGCAAGGAGGCTGGCATGCG GGAGCAGTGCGTGCTCTCTGAGGAGCAGATTCGGAAGAAAAGGATTCAGAAGCAGCAACAGCAGCAGCCA CCACCCCCATCTGAGCCAGCAGCCAGCAGCTCAGGCCGGCCAGCGGCCTCCCCTGGCACTTCGGAAGCAA GCAGCCAGGGCTCCGGGGAAGGAGAGGGCATCCAGCTGACCGCGGCTCAGGAGCTGATGATCCAGCAGTT AGTTGCCGCGCAGCTGCAGTGCAACAAACGATCTTTCTCCGACCAGCCCAAAGTCACGCCCTGGCCCCTG GGTGCAGACCCTCAGTCCCGAGATGCCCGTCAGCAACGCTTTGCCCACTTCACCGAGCTAGCCATCATCT CGGTCCAGGAGATTGTGGACTTTGCCAAGCAGGTGCCAGGGTTCTTGCAGTTGGGCCGGGAGGACCAGAT CGCCCTCCTGAAGGCGTCCACCATTGAGATCATGTTGCTAGAAACAGCCAGACGCTACAACCACGAGACA GAATGCATCACGTTCCTGAAGGACTTCACCTACAGCAAGGACGACTTCCACCGTGCAGGCTTGCAGGTGG AATTCATCAATCCCATCTTCGAGTTCTCGCGGGCCATGCGGCGGCTGGGCCTGGACGATGCAGAGTATGC CTTGCTTATCGCCATCAACATCTTCTCAGCCGATCGGCCTAATGTGCAGGAGCCCAGCCGTGTGGAGGCC CTGCAGCAGCCCTACGTGGAGGCGCTCCTCTCCTACACGAGGATCAAGCGCCCACAGGACCAGCTCCGCT TCCCACGCATGCTCATGAAGCTGGTGAGCCTGCGCACCCTCAGCTCCGTGCACTCGGAGCAGGTCTTTGC ATTGCGACTCCAGGACAAGAAGCTGCCGCCCTTGCTGTCCGAGATCTGGGATGTGCACGAGTAGGGGCAG CCACAAGTGCCCCAGCCTTGGTGGTGTCTTCTTGAAGATGGACTCTTCACCTCTCCTCCTGGGGTGGGAG GACATTGTCACGGCCCAGTCCCTCGGGCTCAGCCTCAAACTCAGCGGCAGTTGGCACTAGAAGGCCCCAC CCCACCCATTGAGTCTTCCAAGAGTGGTGAGGGTCACAGGTCCTAGCCTCTGACCGTTCCCAGCTGCCCT CCCACCCACGCTTACACCTCAGCCTACCACACCATGCACCTTGAGTGGAGAGAGGTTAGGGCAGGTGGCC CCCCACAGTTGGGAGACCACAGGCCCTCTCTTCTGCCCCTTTTATTTAATAAAAAAACAAAAATAAAGTT TGAGTACAAGCCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829527 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829527 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11706 same embryo
	EMAGE:11705 same embryo
	EMAGE:11707 same embryo
	EMAGE:11704 same embryo
	EMAGE:11708 same embryo
	EurExpress:euxassay_006874 same experiment
	MGI:4826781 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source