EMAGE mouse gene expression spatial database

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EMAGE:11704

Ikzf5 IKAROS family zinc finger 5 ( MGI:1914393)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006896. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006896_01	euxassay_006896_02	euxassay_006896_03	euxassay_006896_04

euxassay_006896_05	euxassay_006896_06	euxassay_006896_07	euxassay_006896_08	euxassay_006896_09

euxassay_006896_10	euxassay_006896_11	euxassay_006896_12	euxassay_006896_13	euxassay_006896_14

euxassay_006896_15	euxassay_006896_16	euxassay_006896_17	euxassay_006896_18	euxassay_006896_19

euxassay_006896_20	euxassay_006896_21	euxassay_006896_22	euxassay_006896_23	euxassay_006896_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11704_wholemount_strong.wlz

11704_wholemount_moderate.wlz

11704_wholemount_weak.wlz

11704_wholemount_possible.wlz

11704_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11704_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 18 19 20 weak expression: see section 13 14

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24 weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9149
Entity Detected:	Ikzf5, IKAROS family zinc finger 5 ( MGI:1914393)
Sequence:	sense strand is shown >T9149 CAGCTGCGACGCTGACGAACGCTGAGGCGGTGGCGGTGGCGGCGACGGCAGTGAAAAGGGCTGTGGGGAT TCCTCGGTGCGGTTGTCAGGAGATATAACAGACTTTCAACTTAAAGAAAGAAAGAAAAAAAAAAGCACCA AGATGGGTGAAAAGAAACCAGAGCCTTTGGACTTTGTAAAAGATTTCCAGGAATACCTGACCCAGCAGAC GCATCATGTGAACATGATTTCGGGATCAGTTAGTGGGGACAAAGAAGCAGAGACTCTTCAGGGAGCTGGA ACAGATGGCGATCAGAATGGCCTTGATCACCCGTCTGTTGAAGTTTCCCTGGATGAAAACTCAGGAATGT TAGTAGACGGGTTTGAGAGGACCTTCGATGGGAAGCTCAAGTGTCGGTACTGCAACTATGCCAGCAAAGG AACGGCCAGGCTCATTGAGCACATCAGGATCCACACAGGCGAGAAGCCTCACAGGTGTCACTTATGTCCA TTTGCATCTGCTTACGAGCGCCACCTGGAAGCCCATATGCGCTCTCACACTGGGGAAAAACCATACAAAT GTGAATTGTGCTCCTTCCGCTGCAGTGACCGAAGTAACCTGTCCCACCATCGAAGGCGCAAACATAAAAT GGTACCAATTAAAGGTACCAGGTCTTCCTTAAGCAGCAAGAAAATGTGGGGGGTTTTACAAAAGAAAACA AGCAATCTGGGGTATAGCAGAAGAGCACTAATCAACTTAAGTCCACCTTCCATGGTGGTCCAGAAACCAG ACTACCTTAATGATTTTACACACGAAATCCCAAATATTCAGACTGACTCCTATGAAGCTATGGCTAAAAC TACACCAACTGGTGGCCTGCCAAGGGACCCCCAAGAACTCATGGTTGACAACCCTTTGAATCAGCTCTCT ACTTTAGCAGGACAGTTGTCCAGCTTGCCACCAGAAAACCAAAACCCTGCCTCTCCTGATGTAGATGCCT GCCCTGATGAAAAGCCTTTCATGATTCAGCAACCCTCTGCCCAAGCAGTTGTCTCTGCTGTGTCAGCAAG TATTCCTCAGAGCTCCTCCCCCACAAGTCCGGAACCGCGGCCATCGCATAGTCAGAGGAACTACAGTCCA GTGGCAGGGCCCAGCAGTGAACCAAGTGCCCACACCAGTACTCCCAGCATAGGAAACAGCCAGCCAAGCA CTCCAGCTCCAACCCTGCCGGTGCAGGATCCTCAGCTTCTACACCACTGCCAGCACTGTGACGTGTACTT TGCAGACAACGTCCTTTACACTGTCCACATGGGGTGCCATGGGTATGACAGCCCCTTTCAGTGTAACGTA TGTGGGTGCAAATGTAAAGACAAGTACGATTTTGCCTGTCATTTTGCAAGAGGGCAACACAACCAGCACT GAACACAATCAACATTGTTCTCACTTGGTTTGGCCTTTTGGGAGTTTGTAGTTTGGTTTTTAGTTTGATC ATCCCTAATAAGGTATATGCTAATTTAAAATTTATACATTCTAGTCTATAAAATAGAAGTTTGATAGTGA AAAAGGGTTTTGTTAACTTTTCATAAAAGTCTGGTCAGGGTTATTTATGCATTAGATAGTTAGATGCATT GCTGTCTTTTTCATTTACACCTATGAGAGTTAAAGTACAGTTGTAATCAGTTTCCTACACTTGTCCATAA GGATTCCCAGGGTTATCTGTACTCCTGATGCTTCAGGATATACATTACAGCTAATGTTCTTTCTGCCATA TGTCAAAATGGCACACTTCTCATAAAATCTTTTATAACATTTCAGTGTTTATAGACAAACTGCCTTTAAG TTTGCTCCTAATTAAAATACTATTTTCATTCACATAACTTAAGTTGAATTAGTTTCATTACAACCAAAAC CAGTAGTTATTTCAGTGTAATAAATATTTCTCTTCAAGGAGAAGCATAAAACAGTTCTTTCAAATTTAAA TTATAAAATGAAAATCTTGGAAGTTGAAATTTGGGGCTAAATTGAAAATTGTGTTTGGTATTTGAGATAT CTGTTCATTTAATGTGTAAACATGTTAGTTTTTAGCTTTTCATTTATATATGTATTTATATATTTTGAGT ATTATTTTAAAGCCACAGGTAAATATACTAGAGTGCTAGATGCCTTTTTTCAATCTGATCTTTCATGTAA ATTCTGCATTCACCTGTTACTGGTGTCAGGTAAGATTGACAGCCACACTGATTATTTTATTCTTCCCAGT CTTTGATGACCAAAGAGGAAGTGAGCGCATCATCCCACTGAGATGCTTCCAGAGCCTCCGAGGCTCCTTT CCTGCCCTCTGTGCATAATTGCCCGTAATGTCCACGAAGGGTTGGTCTAGAAACCCAGAGACGTAATAAG TGAGGAAACAGCTACAAACCCAGAGCATGCCCAGACAGAGCCTCCTTGCCTCTCCAGAGCAGAGCAGGCC ATGGAAAGTCCTCCCTGTGAGTCTTTATGGACAGAGACGGTGGGATCGTCACCACAGTGGGAAAGGATAA ATAGAGTTATTATGAAGCACATTTAAATGTGTCATTTAACTTACTTAGAATTAGAGTGGTTCATTTTATA TAGGAAGCCATTATTTTTGTATTGTGTAATAAGTAGCTTACTCTGAAGAGAGCCTGGCAAAACAATAAAA CATTGTCACTTACTATTGGTTTCTGTGTACCTTGCAAATTTTTCATTTTTATTTGGTTAATAGCTTGAAA ATATTCTCAATTGGCTTCTTAAAGAAATACCCTGTCAAGCATTTATTAACCAAAAAATAGCCTCTTACAG TAAATAAGAGGGAACTATGAGAGTGTCTACCCGTGAAGCCCCAAGTGCCCTGGTAATGGACCTGCTCTCC CTCCCTGTGCTGGAAAGCCATTCCGAGGGTGCCTGTGCGTACACAGCCAGTCTTGGCTCCTCCCAACTGC ACTGTTAAAGCGCAGCCCCGAAGACACTTTCTAGTGATTTTGGTCTGTTGCTTAAATCATTTACTAGATT AGTTTTAGGGCAACACTTTCCAAATACATAACTTGACTGTTGTGCTTATCAGCTGCCAGAAAGAAACAGG TTATTGTTGGGAAACCTTGCTGTTTTTAAAGTTTGATTCAATTAAAAGCAATACTTCCAAATAGACAGTG AAAACTTCCCAAAAGAGGAAGAAGCCCAGTGTACTGCCGCACATCTGTAGTCCCAGCTGCTTGAAGCAGA GGCGGGAAGAGTGCTAGAGCCCAGGAGGTCCCTGCCAGGCCGGGCAACACTGCAAGACACAGTCTCAAAG GCCAAGCAGCCGAAAGGGCTCTCCTCACAGCCAGCTTTATCACCTCTACGTTCTGGGCCAAACAGTGACA TGGGCATTTTGGTTTTCCTAACAACTAACGATGAATGTTCTGAAGGTACATTTCATTTTAAATACGGAAA AAACTTTTCATAATTACAGACAAGAAGGAAAAACCCCCGATTTTCCAATAATATAAAGAGCACAGTTGGT CTTCCAAAGGTGTCCATGTGTGAAGCTACAAGTACTTCGAAATGCACTAAAATTTTGAAGCAAATCTACC TTAGGAACTTTTTTGAAATTGTACTTTTAAAGGTTTAGGCTTTTGCTTAAATATTCTGAGTTAAATTCTG TACTAGGAAAGATGTTGGATTCTTTCCTTAAATACTAAATAAGTTCCTCATCTTATTTTTAATCAAATGT TTTATAGAAATGAGTTGAGAAAAGTTGACCATGCACTATTTATAGTACTTTGCCATAACAGGCAATGTTC TAAAACTAAATTTATTTTTGTTTAGTGAACATGAGTTTAGATTTTTAAAGTTGGTAATTTATCTCCACTA ATATTTTTTTAAAGAAACTGTGAAGAGATTAACAGAATAATTTTATTTCAGATTTTACTAATGTAGTGTG TAGCTACAACTTGACATTCAAGTGAAGACTAGACTTCTGTTTTGAAATTTTTCCATCAGGTAGTTTCAGT GGTTTCATTTTAGAAATGTGTTTGCAGATGTTTGACAAAGGGAATATTACTAAAAAAGAAAAGCAAAAAA AAAAAAAACTTATCAAATGAAGAATTGTTAGAAAAATTTAAATTCCCTGCTAAAGACATTTTTGTTAGTC TTAGGGGGTAGGTATTTGCTTTCTAGAACTTGTTTTTGTTGATAGTATTTGCTTTCTAGAACTTGTTTTT GTTGATAGCTGGATGTATGGAACCTGTTTTCTATGCGAGGTGCAAGTCTTGCACTACAGTTGTAAGATAT ATTATATTGTTTAGGGATCAAGAAAGCAATCTATGCTGAAGTAACTATAGTATGTAGTTCCTATTGTGAA TAATGCTTTGCTTTTGTAATGTATTAATAAAAAGTAACACTTTCTAATGAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826723 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11706 same embryo
	EMAGE:11705 same embryo
	EMAGE:11703 same embryo
	EMAGE:11707 same embryo
	EMAGE:11708 same embryo
	EurExpress:euxassay_006896 same experiment
	MGI:4825556 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source