EMAGE mouse gene expression spatial database

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EMAGE:11717

Sgtb small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta ( MGI:2444615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007090. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007090_01	euxassay_007090_02	euxassay_007090_03	euxassay_007090_04

euxassay_007090_05	euxassay_007090_06	euxassay_007090_07	euxassay_007090_08	euxassay_007090_09

euxassay_007090_10	euxassay_007090_11	euxassay_007090_12	euxassay_007090_13	euxassay_007090_14

euxassay_007090_15	euxassay_007090_16	euxassay_007090_17	euxassay_007090_18	euxassay_007090_19

euxassay_007090_20	euxassay_007090_21	euxassay_007090_22	euxassay_007090_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11717_wholemount_strong.wlz

11717_wholemount_moderate.wlz

11717_wholemount_weak.wlz

11717_wholemount_possible.wlz

11717_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11717_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 11 12 13 14 15 16 17 18 moderate expression: see section 19 20 21

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 15 16

pons mantle layer

moderate

regional

moderate expression: see section 05 15 16

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 17

trigeminal v nerve

moderate

regional

moderate expression: see section 08 15 16

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

weak

regional

weak expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 17 18 weak expression: see section 08

neural retina

strong

regional

strong expression: see section 01 20 21 moderate expression: see section 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 moderate expression: see section 06 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4354
Entity Detected:	Sgtb, small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta ( MGI:2444615)
Sequence:	sense strand is shown >T4354 GCGAGCAGCCGGGTGGGCTGAGGGGGCGGCGGCGGCGGCAGCATTGGAGCGGGGGAAGTTTGGGAGCCGT GGCCCCCTCTGCGATCCAGAACACCGCTCAAGCCCCGAGGACCGCGGGGACAGGCGGACCCGCTTCCCGA CGCCCCCGGGCCCGCAGCCGTCATCTCGTGTCCTCTCGTGGAGATATTTAAGCGTCTAAAATGTCATCTG TGAAGCCCCTAGTTTATGCAGTTATTCGTTTCTTGCGGGAACAAAGTCAGATGGATGCATATACTTCTGA TGAACAAGAAAGTTTGGAAGTTGCCATCCAGTGTTTGGAGACCGTTTTTAAGATCAGTCCAGAAGACACA CATCTGGCAGTTTCACAGCCTTTGACAGAAATGTTCACAAACTCCGTCTGTAAGAATGACATACGGCCGC TTTCAAACTCAGTGCCCGAGGATGTGGGAAAGGCGGACCAGCTGAAGGATGAAGGCAATAACCACATGAA AGAAGAAAATTACGCTGCGGCAGTGGACTGCTACACGCAGGCAATAGAACTGGATCCCAACAATGCCGTT TATTATTGCAACAGGGCTGCTGCTCAAAGCAAATTAAGTCACTACACAGACGCAATCAAGGACTGCGAGA AAGCCATTGCAATTGACTCGAAATACAGCAAGGCCTACGGAAGGATGGGGCTCGCCCTCACTGCCATGAA TAAGTTTGAAGAGGCCGTTACAAGCTACCAGAAGGCACTGGATCTCGATCCTGAGAATGATTCGTATAAG TCAAATCTGAAAATAGCAGAACAGAAGTTGAGAGAGGTGTCTAGCCCTACGGGAACCGGGCTGAGCTTTG ACATGGCTAGCTTGATAAATAACCCGGCCTTCATCACCATGGCTGCAAGTTTAATGCAGAACCCTCAAGT TCAGCAGCTAATGTCAGGGATGATGACAAATGCCATTGGAGGACCAGCTGCCGGAGTTGGAGGCCTAACA GACCTGTCTAGCCTAATCCAAGCGGGGCAGCAGTTTGCGCAGCAGATACAACAGCAGAATCCGGAACTCA TAGAGCAGCTGAGAAACCACATCCGGAGCAGATCGTTCAGCAGCAGTGCAGACGAACACTCCTGACTTGA CCAGGACCCTCGCCCCAGTACAAACATTCTGACGTGTACACTTGTAGATAATAGCCAAAAACAAAGTAAC AAAACCCACCCTATACAGAGTCTGAACATAACGGAGGAAAGTCTCCTGTGTAAACCTGAGAGAATACATC TGTTTAGATAATAGTTTACCACAAGCAGACTTGAACGTAATCCAGACAGACTCCTTTGGAAAGCAGTGAT CAGTAGCCTTGTTAAGGGTGAATACAGCCTGGGGACAAGGGTCCATCCATTTTAAAACTTTTATCATCAT ATTTGTATATTAGACTTAGTACAGAGTATATTTATTGAATAGTAGGGCTATTTATTGGAAAGTTCGTTAG CTGCAGGTTTCCCTAGGCAGTGGTCTTGAGCGGGGCGATGCTGGTTTAGAAGACACGTGCTTGGCAGCAC GTCAGAGAGAAGCGCTGGCACGCGCTACCATCAGTGACGCGTGTCTATCGAGAGTGGCACTTGGAAGATA CTGTGAATGCCCAGAAAGCGAAGGCAGTGTGCTCAGGTTTTCTGCATAGATTTTTTAAACTAAGCTTGTC TGTAGCTTCGTGTTTATAGCATTCGCATGGCCAGTTAAGTACATAATGAAACATACTTAGAAATCCTTAA TGATGCTGGTCAGGTCAGTGACATTACTGCCCCATTTAATTATCACAGCTTAAATGTCACAATTTCATTG CTGGAAAACTTACCTAGGGCAGTATCTCGTTACATTTAAAGAAGCCACTGACGTGTCCCCAGATTCCGGG TTTCCTAAGTGGGATTTAATTATGTGCCCATCAAAGACGACGACGACGATGACTATTTTTCTTGACCATT ATTTGATGGCTAACAGAAGCATCCATCTCTAACATTGCTCACTTGTGTAATCCTTTGGTGCAGTTTGGCT TGGCATGTCGGTTCTTATGCTTAACTTTAATTTATTGCAATTTAAACGTAACAGGAGACTTCAACTGTCT TATTTTAAATTGGATGCCACACTGTGCATCTGGCGTGTGCTTAGTAGGAAGAAATGACACGAGCTATCGA TGGGAGGACTCTTAGTGCACTGATGGATAAGAAGGCTCATTTCAAATGGAACTGGCTTATTAACCAAAAA CTTGGTTCGTATCCCATCACTGCATTAGAGAGGAAATAAGTAGGAACAGAGAGTAGATGCCCCATGGAAC AGATCCTGGAGCTGTTAAAGGACAACCAGAGCGCCTGGGAACACAGATCAGTGCATAGTGCTTGCCCAGC ATGTGTGAGGCCCTGGGTTACCCCTTCCCTAGTACTGCATGCAAAGGGAATGGCGGTGAGTCCCAGCACG TGGGAGGTAAGGGTCAGGACCAGGAATTCAGCATCACTCTTGGCTACACAGTGAGTACAATGCTAGCCTG GGCTACAGGACCCCTGTCTCGAAAACAAAAACCCCCAAACACCCCAGTAGAAAAAAAACCCGACCCAAAC AACAACAAAACAGACAATTAGAAAACCTCATGACTTGCTCTAGATTTCTGTATTTAACAACTTTGGGAAA CAAATCTTTGTGAAGAAGATAAGCTGAATAGGCCTGTCTTGTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527683 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527683 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11720 same embryo
	EMAGE:11716 same embryo
	EMAGE:11719 same embryo
	EMAGE:11718 same embryo
	EMAGE:11721 same embryo
	EurExpress:euxassay_007090 same experiment
	MGI:4828031 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source