EMAGE mouse gene expression spatial database

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EMAGE:11725

Rnf111 ring finger 111 ( MGI:1934919)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006587. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006587_01	euxassay_006587_02	euxassay_006587_03	euxassay_006587_04

euxassay_006587_05	euxassay_006587_06	euxassay_006587_07	euxassay_006587_08	euxassay_006587_09

euxassay_006587_10	euxassay_006587_11	euxassay_006587_13	euxassay_006587_14	euxassay_006587_15

euxassay_006587_17	euxassay_006587_18	euxassay_006587_19	euxassay_006587_20	euxassay_006587_21

euxassay_006587_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11725_wholemount_strong.wlz

11725_wholemount_moderate.wlz

11725_wholemount_weak.wlz

11725_wholemount_possible.wlz

11725_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11725_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8105
Entity Detected:	Rnf111, ring finger 111 ( MGI:1934919)
Sequence:	sense strand is shown >T8105 GCCGCGGCGGCCGGCCCGGGTCGGCGACAGGGGAATGACCGCGGGTCCCGGACGGCGCGGAGTGGCTCAT TGTGTCCGGGCAAGAGTCCGCCCGCGGACACACCCACGGCCTCTTGGGAGGGGATAATTGATCGGAGTCA GCAGTGGTGCGGTGGAAATATCGGTTTTTGTTCTGGAGAAATAGAGGCCTTCCCTGAAGTTTCCCATGTC TCAATGGACTCCTGAATTTAACGAGCTCTACACCCTAAAAGTGGCTATGAAGAGTGGGACTCCTGATGCG CCCACTACACAGGAGAGTCTGAAGGCGGTCCTTCTGCACCCACAGCCCCTAGGGGCCACCAAGAGCTTCC CTGCAGAAGTGGAGATGATTAATAGCAAAGTGGGGAATGAATTCTCTCATCTGTGTGATGATTCTCAAAA ACAAGAGAAGGACATGACTGGCAACCAGCAAGAACAAGAAAAAAGTGGTGTTGTGAGAAAAAAGCGCAAA AGCCAGCAGGCTGGCCCCTCATATGTGCAGAACTGTGTCAAAGAAAATCAGGAAATCCTAGGGCGCAGAC AACAGCTAGAGACACCGAGTGATGAGGATAATGACTCTTCCTTAAGTGAGTGTCTGTCTTCTCCCTCATC TAGTCTCCATTTTGGGGGCTCTGATACAGTGACTTCAGATGAGGATAAAGAAGTCTCGGTGAGACATACC CAGCCTGTTCTGAGTGCTAAAAGTAGAAGTCACAGTGCAAGGTCTCACAAGTGGCCTCGAACTGAGGCAG ATCCTGTGCCAAGTTTGTTAATGAAAAGACCTTGTTTTCATGGCAGTGCACTAAGGAGAGTCACATGCAG AAAGAGGCTGGTGAAGAGCAGCTCCTCGCAGAGGACACAGAAGCAGAAGGAGAGGATGCTAGTGCAGAGG AAGAAGCGGGAGGCGCTAGCTCAGAGGAAGTATGCTCTTCTCTCCAGCTCCAGTAGCTCCAGTGAGAATG ACCTCAGCAGCGATTCCTCGTCCAGCTCCTCCACCGATGGAGAAGAGGACCTGTGTGCATCTGCCAGTGA GAACCCCAGCAACCCCGCTGCTCCCTCAGGAAGTATTGATGAAGATGTTGTGGTGATAGAAGCCTCCTTC ACTCCCCAGGTCACTGCCAATGAAGAAATTAATGTTACCTCAACTGACAGTGAAGTGGAGATTGTTACAG TTGGAGAAAGCTATCGGTCTCGTTCAACCCTTGGACACTCCAGATCTCATTGGAGCCAAGGTTCAAGTTC GCATACAGGACGGCCACAGGAGTCTCGAAACCGCAGTAGGATCTCTACTGTTATACAGCCCCTGAGGCAG AATGCAGCAGAAGTTGTGGACCTTACTGTTGATGAAGATGAACCTACTATAGTACCAACCACTTCTGCTA GAATGGACTCACAAACTACTAGTGCTTCCATTAACAATTCAAATCCATCTACCTCTGAGCAAGCCTCTGA TACTACTTCAACTGTGGCCAGTAGCCAGCCTTCCACAGTGTCAGAGACTGAAGCTACTCTTACAAGCAAT AGTGCAACTGGTTCTTCTGTGGGCGATGACGTGAGGAGAACTGCATCTAGTGCTGTCCCAGAAAGTGGTC CTCCTGCAATGCCAAGGCTACCTTCTTGCTGCCCCCAGCACTCACCATGTGGAGGGACATCACAGAGCCA TCATGCATTAGCACACCCACACTCAAGTTGTTTTCAGCAGCATGGCCACCATTTTCAGCATCACCACCAT CACCACCATACTCCCCACCCAGCTGTCCCAGTTTCTCCTTCCTTCAGTGACCCTGCTTGCCCAGTGGAAA GACCTCAAGTACAGGCGCCTTGTGGAGCAAATAGCAGCTCTGGTTCCAGCTACCATGACCAGCAGGCATT GCCAGTCGATCTGAGCAACAGTGCTCTCAGGACTCATGGAAGTGGGGGTTTCCATGGTGCATCTGCTTTT GACCCCTGCTGCCCTGTCACATCTTCCCGGGCTGCAGTCTTTGGCCATCAGGCTGCTGCTGCCCCGACTC AGCCATTATCAATAGATGGCTATGGCTCCAGCATGGTTGCCCAGCCCCAGCCCCAGCCCCCTCCACAGCC TTCTCTTTCGTCATGTCGGCACTACATGCCACCTCCGTATGCTTCCTTGACAAGACCACTGCACCATCAA GCCTCAGCTTGCCATCACTCTCATGGAAATGCCCCTCCCCAGACTCAGCCTCCCCCTCAAGTGGATTACG TGATTCCGCATCCTGTCCATGCTTTCCATTCTCAGATATCTTCTCATGCAGCATCTCACCCTGTGGCACC ACCGCCGCCAACACATTTAGGCAGTACAGCGGCACCCATCCCTCAGCATCTTCCTCCTGCTCACCAGCCG ATTTCACACCATATTCCAGCCCCAGCACCTTCAGCCCAGAGACTGCACCCTCATGAAGTGATGCAGAGGA TGGAAGTTCAGAGGAGGAGGATGATGCAGCATCCAACGCGGGCACATGAACGGCCCCCGCCCCATCCACA TAGGATGCACCCAAACTATGGCCATGGGCATCATATTCATGTGCCTCAAACCATGTCTTCACATCCTCGC CAGGCTCCAGAGAGAACTGCCTGGGAACTGGGGATTGAAGCTGGAGTAACTGCAGCTACCTACACACCTG GTGCTCTGCACCCTCACCTGGCCCATTACCACGCCCCGCCTCGACTTCATCACCTCCAGTTAGGAGCACT TCCTTTAATGGTTCCTGACATGGCAGGCTATCCTCACATCCGTTATATTTCTTCAGGATTGGATGGAGCA TCATTCAGAGGTCCTTTTAGGGGCAATTTTGAGGAATTGATTCATCTGGAAGAAAGATTAGGAAATGTCA ATCGTGGAGCCTCCCAGGGAACAATTGAAAGATGCACATATCCACATAAATATAAAAAGAGGAAACTGCA CTGCAAACAAGATGGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGTCTATT TTAGAGGAAGGTGAAGATGTGAGGCGCCTTCCATGTATGCACCTTTTCCACCAAGTGTGTGTTGATCAGT GGCTGATCACCAATAAGAAGTGCCCCATATGCAGAGTGGACATCGAGGCCCAGCTGCCAAGTGAAAGTTG ACATTGTGTTTCAGAACTCTTGGTGTCCCTCTCATTCCTATCCTTCCTGGTACTGCAGTCAACCAAAGAT GGCATGACTTACCTGCGCAGATCTGGAAGCCTTGAACTCGGAGTGCTGACTCTGCTACATGGTACAGCTA CCGCTAGACCTACAACTTATGTACACAGTTGATTTTGATGTATTTATAAAAGCTTTTTTTTTCCCTAGAT TTGACATTACTTCCTGTATCATTTTACTGTATTTTTGCATGATTCCTTGTATTGCATTTCTTTGCACATA TTATGGGCTTGTGACCCTAAACTTGCAGGCAAGATTAGCTGCTTTAGTAAGTAGTATTTTGTGGTCTTTT TTTTGTTTTTTGGGTTTTTTTGTTTTTGTTTTTGTTTTGTTTTTGTTTTTTGTTTTTTACATAGTACCTT GAAAATTATGAATTTTTTATCCATTACTAACCTTTAATTTAATCAATCATGTACTTTAGTTTAATGTATA AAGATTTTCTAGGAAATGATATTGTGTATTAAGACATTCCTTAATTAAATTAGGACAAAATGGCTGCTGT ATATTTACAATATGGAATTCTGAGTTAAGTACCGCCCTTCATACTGGGAACAGAATGTATGTAACCCATG TCAATCAGATGCAGGTGGTACCCACACGACCAGAGTGATCAATAGGAATTTTCTTGGTGTTCCTTTCTTC CAGCACAGTGCAAGATAGAGTTGCTGTTGATGCCATATGTTTAGACTGCTGTTCTGACCCTATTTATCTT TCTCTCTGCCATTTGGAACTTTATTCCCTAACCCAGGTTTTGCTGCTGTGAAACAGCGTTGATGAACACT AAATATTAATCTGAGATAGCTGGATTGTATATACTTGCAGATTTAAAAATATAGGGAAATTGAAAAAGAC ATGTAGAATTTTGTTAGGTCTTCTGCTAAGCACGAATAAGATATCTGCTTACATTAATTTTTATAAAGTC ATTCAGTCAGAATTTAGGATTCAGTCATTGGCAGGTATCTATGCATTCGTAGAACTTCTAAGGGTCCCAG GATCACTTTTAAGGGATTTTTTTTTTATAGTTTAAATAAAGTCAGCTGAATCTACATGTCTCTTGTTTTA TTTCTCTCTAAACTTGAAAACAGTAAATCTGCAGATACTGTGAGGCACAGATTATATTGTCAAGCTACTG TTGCTATGGTTATAGACACCCACTTCAGACATTACCAAGAGTCGATCACTGACTTAAAAATTTTAATTTC TATAGTTAAGATTTACAACATAATATAGAGTGAAAGTTAACATACTAACATTTCTCCTTTGGAGGAAGTT TTAATCCACTTCAGGATGCATATTAGCAAGATACTTTCATATACAGGATAGCCTAATTTTATTTGTTTAA ATATGCTTAATATGCCCAAGATTGCAAATGCATTCAGTCAGTAATACCACTGTCTGTATGTGGAAGACAT GTTCCCATGGATCATATGTGAAGATGTCAATAAGCTTGCATTAAGCCACCTGCTTTGTAAGTGGATTGAT TAATAAATAACTTATATTTCTAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30094289 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30094289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11726 same embryo
	EMAGE:11727 same embryo
	EMAGE:11728 same embryo
	EMAGE:11724 same embryo
	EMAGE:11722 same embryo
	EMAGE:11723 same embryo
	EurExpress:euxassay_006587 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source