EMAGE mouse gene expression spatial database

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EMAGE:11727

Cry2 cryptochrome 2 (photolyase-like) ( MGI:1270859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006684. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006684_01	euxassay_006684_02	euxassay_006684_03	euxassay_006684_04

euxassay_006684_05	euxassay_006684_06	euxassay_006684_07	euxassay_006684_08	euxassay_006684_09

euxassay_006684_10	euxassay_006684_11	euxassay_006684_12	euxassay_006684_13	euxassay_006684_14

euxassay_006684_15	euxassay_006684_16	euxassay_006684_17	euxassay_006684_18	euxassay_006684_19

euxassay_006684_20	euxassay_006684_21	euxassay_006684_22	euxassay_006684_23	euxassay_006684_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11727_wholemount_strong.wlz

11727_wholemount_moderate.wlz

11727_wholemount_weak.wlz

11727_wholemount_possible.wlz

11727_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11727_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8107
Entity Detected:	Cry2, cryptochrome 2 (photolyase-like) ( MGI:1270859)
Sequence:	sense strand is shown >T8107 CGGCGATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGCGCAATCGATGGGCGCGGACGGCGCGTC CTCGGTGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGCTAGCTGCCGTGCGCGGG GCGCGCTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCTGTGGGCATCAACC GATGGAGGTTCCTACTGCAATCTCTGGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCGCGTCTGTT TGTAGTCCGGGGACAGCCAGCTGATGTGTTCCCAAGGCTGTTCAAGGAATGGGGGGTGACCCGTTTGACC TTTGAATATGACTCTGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCTG GCGTGGAGGTGGTGACTGAGAACTCTCACACCCTCTATGACCTAGACAGAATCATCGAACTGAATGGGCA GAAACCACCCCTTACCTACAAGCGCTTTCAGGCCCTCATCAGCCGCATGGAGCTGCCCAAGAAGCCCGCG GTGGCTGTGAGCAGCCAGCAGATGGAGAGCTGCAGAGCTGAGATCCAGGAGAACCATGACGACACCTATG GCGTGCCTTCCCTGGAGGAGCTGGGATTCCCCACGGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGA GACAGAAGCTCTGGCCCGCCTGGACAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCT CGGATGAATGCCAATTCCTTACTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGATGCCTCT CCTGCCGCCTCTTCTACTACCGCCTGTGGGACTTGTACAAGAAGGTGAAGAGGAACAGCACACCCCCCCT CTCCTTATTTGGACAACTCCTGTGGCGAGAATTCTTCTACACAGCGGCCACCAACAACCCCAGGTTTGAC CGAATGGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGCAACCCCGAAGCCCTGGCCAAGTGGG CCGAGGGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCA CCACCTGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGCGGGGACCTCTGGGTCAGCTGGGAGAGCGGG GTCCGGGTATTTGACGAGCTGCTCCTGGATGCCGATTTCAGTGTGAATGCAGGCAGCTGGATGTGGCTGT CCTGCAGTGCTTTCTTCCAACAATTCTTCCACTGCTACTGCCCTGTGGGCTTCGGCCGACGTACAGACCC CAGTGGGGACTACATCCGGCGGTACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATACATCTATGAGCCC TGGAATGCCCCCGAGTCAGTTCAGAAGGCTGCCAAGTGCATCATTGGCGTGGACTACCCACGGCCCATCG TCAATCATGCAGAGACTAGTCGGCTCAACATTGAACGAATGAAGCAGATCTACCAACAGCTGTCGAGATA CCGGGGACTCTGTCTATTGGCATCTGTCCCTTCCTGTGTGGAAGACCTCAGTCACCCTGTGGCAGAGCCT GGTTCAAGCCAAGCTGGGAGCATCAGCAACACAGGCCCCAGAGCACTATCCAGTGGCCCAGCTTCCCCCA AACGCAAGCTGGAAGCAGCCGAGGAACCTCCTGGTGAAGAACTGACCAAGCGGGCTAGAGTGACGGAGAT GCCTACCCAAGAGCCAGCAAGCAAGGACTCCTGAGACTGGAGAGCCATCGCTCCGTGAGCAAAGCCCGGG TGCCTGAGCTACCATGGCCACAGAGTGCAATCTGCAGAGAAGCCAATCACCAACAGACGGAGCGAGCGAG TGTGTGCGTGTTTGCAGAGGAAGGTGTGGTGTGCCATTTGTGTGTGCATGCATCTGTTTACACTCTCATG ATCCTGAATGTTGCCTGCGCTGGAGGAGTCCCTAGATCGTATCTTCTTACCAGGGCTGCTTCTTGACCTC CAGACACAAGACTAGAAGCCACAGTAGTGACTTTCAGCCCAAATCTGTCCCTGGGAGCCCCTTCCTTGTC CCAGTGAGGTGGTAGTACCCTAGCTCAAATTCTGGCCTCTGCCTCCCTTGATTTTTCTTCCTCCCTCCTC CTTTCAGACAAGGAGCTGGAGGGCCACATTTTTTGACTCTCATCTGAAGCATAGAGTTTCAGAGGCAGCC AGAGTCCTGCTGACTTAGTTCCCATGTTCTGACACTAGAACCTGAGCAGGCTGGAGTACCAGTCTTGTAA AACTCCATACTACTAGCAGGCTTCCCTGATGTCTGAGACAACTGACTGGTGTGCTGTGTGCCCATGCTGC TTATGTCAGCCTCAAACAGCAAATCTGTCTGTGTAGAACCTTCTAGAAGCCAAGTGGAGAATAGACTCCC AGGCTTCTGTCCCTTCCCCCGCAGCTAAGGCCCAGTCTCCCACCTTCTAGAAGCCGTGAACAGGCCAGGG TCTGAGCCGGTGGAAGAAATCCTCGTCCCTTGGCTGAAGGCCCCTGGTTCCTCTGGCCATGCTCCTGACA CGACTTGCTCCAGCAAGTCCTGCTGCTACCCAGCCAGGCAAGCGGGACAGTCTGCTCTGCAGGCTTCCAG GGCCGGCCCTGGACCAATCAGCACCCACACTGCAGTGTCAGTCCAGGACTGAGCTTCAGGGGACTCAGAG AGACTGAGAGTGAAGCACTGCCTGGGGCAGTTGGTGGAAGGTCAGAGATCACACCTGGCACACCAACCTC TTCTCAGAGCAGCAGTGGAAGGTTTGGCCTAGAGAAGTCAAGCCTCACTTTCCAGGGGAGCCAACTCTCC CGCCCACTGCTGGGTCTTATGGAGAAAGAAGTTATGAAAAGAAGGATCTGGGGGCACCTGAGCAAGTCTT CCTTCTACCCCGTGGCCTGCACTTGAGCCACAGTGTATGTGTGGGGAGAAAGAGAAAGAGTGTGTATGTG CGTGAGAGAGTGTGTGTGTGTGTGAGAGAGAGAGAGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGCTGTTTGGATTTTTGTTCTCACATGTAACATTAAGCTGGCCTCTGGGCCTTTTCCTTT CTACCTCCCCTGTGACCTTTCCTAGCCTCAGAGTTGTTAATGCCCTTGGCCCTGGCCTTTATCATGTCAG ACCAGAAGCCTGGGGTCAGACTCGCCTCTCCAGCTGTCTAGTACATCTGACAGGCTTCTTTTTGAGATGG CCTCAGGTTTTCTCAGCAGAGAGCTGCCTTTAGTCCAACTGTTTGTGTCCATCATTCTGACTAGAAGCAT CCTATGATTGATCGGGTGAAGAAACAGGGCACCTGTGATGTCCATGTTTAGAGTTCTGGGGTGTAACCTC CCTGCTCCTTAGCCCAGGCCAAGAGGAAGGAGCCAAACCGCCTTGCTGGCGAGAAGGTAGAGTGGGCCTG AAAAACCAGTCTCAGGAGCAAAGGCTAATTCTGCGTCCAGGATTTAGTTTGCAACATCTTCAGCTGTGGG GACAATGGCCTTCTCCGCTTTTTTTGATGATGGTTCTCTTCACAAGGTATAAGTTGGCAAGATCATCTGT CCCCCCACCTCCTTGACAAGCTGGCCCATTCCCAGGCCAGCCTTCCAATGCAAGGAGCAGTCTACACCCA CAGCTGAGATAGAGTGGCCTGGAGCCTTGGGGTGACTTCATGCCTCTTTTTGCCTAGCCTGAGTGGCCCT GAGGACTGTCACAGAACAGTGCCCCATGTCCTCCTCCTGGGACCTAGCATGGGGAAGAGATGGTTGCAGG CAAGAAGCACTTTACAGAGTTCCCTATTGCTGGGAAGGTTGTGTTTCTCCCACAGTTTGTTTGTGAATAT TTACCTGTTTTATAAATGTCTGATCCTGTCTGAGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30103169 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30103169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11725 same embryo
	EMAGE:11726 same embryo
	EMAGE:11728 same embryo
	EMAGE:11724 same embryo
	EMAGE:11722 same embryo
	EMAGE:11723 same embryo
	EurExpress:euxassay_006684 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source