EMAGE mouse gene expression spatial database

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EMAGE:11739

Prpf39 PRP39 pre-mRNA processing factor 39 homolog (yeast) ( MGI:104602)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007127. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007127_01	euxassay_007127_02	euxassay_007127_03	euxassay_007127_04

euxassay_007127_05	euxassay_007127_06	euxassay_007127_07	euxassay_007127_08	euxassay_007127_09

euxassay_007127_10	euxassay_007127_11	euxassay_007127_12	euxassay_007127_13	euxassay_007127_14

euxassay_007127_15	euxassay_007127_16	euxassay_007127_17	euxassay_007127_18	euxassay_007127_19

euxassay_007127_20	euxassay_007127_21	euxassay_007127_22	euxassay_007127_23	euxassay_007127_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11739_wholemount_strong.wlz

11739_wholemount_moderate.wlz

11739_wholemount_weak.wlz

11739_wholemount_possible.wlz

11739_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11739_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4492
Entity Detected:	Prpf39, PRP39 pre-mRNA processing factor 39 homolog (yeast) ( MGI:104602)
Sequence:	sense strand is shown >T4492 GGACATTTGAAGAAGGTATTAAAAGACCTTATTTTCATGTGAAACCATTGGAAAAGGCTCAGCTAAAAAA ACTGGAAAGAATACTTAGAATTCGAAATTGAAAATGGGACTCATGAAAGAGTTGTGGTTCTCTTTGAAAG ATGTGTCATATCTTGTGCCCTCTATGAGGAGTTTTGGATTAAGGAGATGGTCTGCTTGCAACCAGATTTG ACTGCTGCATATGCCAACCTCGTTGCCTCTCTTCGTCCTTCCTTACAGAAACTAGTCTAGTGGTTCAATA AAGTATTTCCTGTTTTTTGTATGTAGTATGCCAAGTACATGGAAAACCATAGCATTGAAGGAGTGAGGCA TGTCTTCAGCAGAGCTTGTACTGTTCACCTCCCAAAGAAACCCATGGCACACATGCTTTGGGCAGCTTTT GAGGAACAACAGGGGAATATTAATGAAGCCAGGATTATCTTGAGAACGTTTGAAGAATGTGTTCTAGGAT TGGCAATGGTTCGATTGCGAAGAGTAAGTTTAGAACGACGGCATGGAAATATGGAAGAAGCCGAACATTT GCTTCAGGATGCTATAAAGAATGCCAAATCAAATAATGAGTCATCATTTTATGCTATCAAACTAGCCCGA CATCTTTTCAAAATACAGAAAAATCTTCCAAAATCAAGAAAGGTACTTTTGGAAGCAATCGAAAAAGATA AGGAGAACACAAAGTTATACCTCAATTTACTTGAAATGGAATACAGTTGTGACCTCAAGCAAAATGAAGA AAATATCCTCAATTGCTTTGACAAAGCCATACATGGTTCATTGCCTATTAAAATGAGAATTACATTTTCT CAAAGAAAAGTGGAATTCCTTGAAGATTTTGGTTCAGATGTTAATAAGCTTCTGAATGCATATGATGAAC ACCAAACACTTCTAAAAGAACAGGATACTTTAAAAAGGAAAGCTGAAAATGGCTCAGAAGAACCAGAAGA AAAGAAAGCACACACAGAAGATTTGTCATCAGCACAGATCATTGATGGGGATTTACAGGCAAATCAAGCT GCATATAATTACAGTGCCTGGTACCAGTACAATTATCAGAATCCTTGGAATTATGGACAGTATTATCCTC CACCTCCAACTTGATGGGAAAATGTAAATTTCAGATGGCTTATATGATATGTGGAATTATTTTTTTTTGA AGGATGTAAACATAGTATATGCTTGTTGTATTTGATAATTTGTCCTCCTAGTTTCTGTGTAACATTTGTC TAGTTAATAGCAGGAAATGTCAATTAATAGTGTATCATAGAAACTTTCCTACCATTTTTAAAATTTACTT TTTATTGGAGAATTATTTTTTTTATTTTTATTTTATCTTTTTTTTTTTTTTTTTTTTGCATTAATTGGTC TAGAATTCTTTTGCAGCTCATGCAGGAGATTTGTAGCCTTAACTGTAGGGAGAAAAAACTGACTGTAAAT CATGTCAGTTAAAAGAGATTTGGGGGGGGGAGAATAAGGGCTGGTTATTGAGAATTTTGCCTCCTATAAA AAGATTTTAAAAAATTTACTTTTAAATCCTAAGGTTGTCTATATGTTACTGGGGAATTTGGCTCTAACTC CAGGAGAGATGAACTGTTGACATGCTCTGCCTTTGAATAGATTATAAGCAAGAAAGTTTAATGTCAGCAT CCCTTTAAGAAATCAAATATCAAAGTATGTTTTAGGAGAGTTTGTATCTAGAATATTCATGTAAAACTTA TGAGCAAGCTTTCATTTTGATCAGACTTATCATTTTTGTAATAAAAATGGAGACTCATGCAGCAATTGTT GATGAATTCATTTGGGGAAGATACACAAAATAGAGATTAACATTGTCTTGAAGTAAATTTTTCTCTAAAT CTATTATCTTCCTACCAGAATGTTTTAACTGTTTTGGTTTTGTTTCAAGTTCCTGTGCCTTTTAATTTCC ACCATGAAGTGTTATATTTTTAATGTCCTTGACAAAGCTAGCCACCTTGATTAGCAGCTCAACTCTTGGG ACAGAGTCACTAGTTTTTACCTAATTAGAATACAGAACACACAATAAATAAGTCAGTCAAGACAATCCAT GACACTTTTATTGAGTCTTCTAGTTTACGAGGTAAAAGGAAAGCAGCTCTCTGGAATACTTTACATAGCT GTAGGAAAATATCTGAGACTTAAAGGGAGTAACAGGATTATGTTTCTCAAAATGTGAGATCAAGTAAACA AGCTTTAACTCTAAATTTTCCAGTTGACTCTTCCCTTACAAGCTCTAATTAGCCACAGCTCATTTTTATG TTTGCTGATGTCCGATCTGAAGTACTGGTTTCAGTCAATGTCTACTAATTCCACTTCAAAAATGAGCTTG GTGTTTGGTGGAATTCTGTGGGAAAAGGTCAAGGAAAATTTGAAAAGTGCATAATAAGAGTTGTAAATCA GATTATGTGCGTGGTAGAGACACAATTAAAAGTTCTAAGGTAAGCCAGAGATTATGCCAGTAGATTTAAG AGGACAGTTTTGTATTTGACTCAGACTAGCTTGAGTTTCTTAAAAGTATACTTGATAATACCTCACTAAT GCTACCAAATTAGCCAGTATCTTTTGAAGGTTGCAACTGCATTCAAAACCTGTATGTTGGCAGAACATGT CATCTACTGTGAATTATAAGACAAGTTTAGGTGTGCTTGGTGGTGCAGTTTTAAATCCCAGCACTCAGAA GGCAGAGGCGGACCTGAGGACAGCTGGTCTGCACAAACTCTAAGAAACAAAAGGTGAGAAGGCTTTATGA TTTCACATGAGATGAATTTAACATTTTGAAGACTACAAAGGACGACCAATCCCAAACCTCACTCCTATCA TCTACAAATTACAGTATCAAAACTAATTTGGCAAACAAGAATGGAATCACCTTTCCAGTTAACTATTAGA TCAGTTTAGTAGAATCATGCCTTTCTGCGTTTTGTAAATGTTGACAAACTGGGCCCTATCATACAAATAC TGTAATCCTTAATAAATGCTTACTAACATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4951324 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4951324 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11743 same embryo
	EMAGE:11740 same embryo
	EMAGE:11742 same embryo
	EMAGE:11738 same embryo
	EMAGE:11741 same embryo
	EurExpress:euxassay_007127 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source