EMAGE mouse gene expression spatial database

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EMAGE:11762

Pomc pro-opiomelanocortin-alpha ( MGI:97742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006904. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006904_01	euxassay_006904_02	euxassay_006904_03	euxassay_006904_04

euxassay_006904_05	euxassay_006904_06	euxassay_006904_07	euxassay_006904_08	euxassay_006904_09

euxassay_006904_10	euxassay_006904_11	euxassay_006904_12	euxassay_006904_13	euxassay_006904_14

euxassay_006904_15	euxassay_006904_16	euxassay_006904_17	euxassay_006904_18	euxassay_006904_19

euxassay_006904_20	euxassay_006904_21	euxassay_006904_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11762_wholemount_strong.wlz

11762_wholemount_moderate.wlz

11762_wholemount_weak.wlz

11762_wholemount_possible.wlz

11762_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11762_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15

hypothalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9962
Entity Detected:	Pomc, pro-opiomelanocortin-alpha ( MGI:97742)
Sequence:	sense strand is shown >T9962 AAACGGGAGGCGACGGAAGAGAAAAGAGGTTAAGAGCAGTGACTAAGAGAGGCCACTGAACATCTTTGTC CCCAGAGAGCTGCCTTTCCGCGACAGGGGTCCCTCCAATCTTGTTTGCCTCTGCAGAGACTAGGCCTGAC ACGTGGAAGATGCCGAGATTCTGCTACAGTCGCTCAGGGGCCCTGTTGCTGGCCCTCCTGCTTCAGACCT CCATAGATGTGTGGAGCTGGTGCCTGGAGAGCAGCCAGTGCCAGGACCTCACCACGGAGAGCAACCTGCT GGCTTGCATCCGGGCTTGCAAACTCGACCTCTCGCTGGAGACGCCCGTGTTTCCTGGCAACGGAGATGAA CAGCCCCTGACTGAAAACCCCCGGAAGTACGTCATGGGTCACTTCCGCTGGGACCGCTTCGGCCCCAGGA ACAGCAGCAGTGCTGGCAGCGCGGCGCAGAGGCGTGCGGAGGAAGAGGCGGTGTGGGGAGATGGCAGTCC AGAGCCGAGTCCACGCGAGGGCAAGCGCTCCTACTCCATGGAGCACTTCCGCTGGGGCAAGCCGGTGGGC AAGAAACGGCGCCCGGTGAAGGTGTACCCCAACGTTGCTGAGAACGAGTCGGCGGAGGCCTTTCCCCTAG AGTTCAAGAGGGAGCTGGAAGGCGAGCGGCCATTAGGCTTGGAGCAGGTCCTGGAGTCCGACGCGGAGAA GGACGACGGGCCCTACCGGGTGGAGCACTTCCGCTGGAGCAACCCGCCCAAGGACAAGCGTTACGGTGGC TTCATGACCTCCGAGAAGAGCCAGACGCCCCTGGTGACGCTCTTCAAGAACGCCATCATCAAGAACGCGC ACAAGAAGGGCCAGTGAGGGTGCAGGGGTCTTCTCATTCCAAGGCCCCCTCCCTGCATGGGCGAGCTGAT GACCTCTAGCCTCTTAGAGTTACCTGTGTTAGGAAATAAAACCTTTCAGATTTCAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30253829 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30253829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11764 same embryo
	EMAGE:11766 same embryo
	EMAGE:11761 same embryo
	EMAGE:11763 same embryo
	EMAGE:11765 same embryo
	EurExpress:euxassay_006904 same experiment
	MGI:4827328 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source