EMAGE mouse gene expression spatial database

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EMAGE:11765

Gh growth hormone ( MGI:95707)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007114. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007114_01	euxassay_007114_02	euxassay_007114_03	euxassay_007114_04

euxassay_007114_05	euxassay_007114_06	euxassay_007114_07	euxassay_007114_08	euxassay_007114_09

euxassay_007114_10	euxassay_007114_11	euxassay_007114_12	euxassay_007114_13	euxassay_007114_14

euxassay_007114_15	euxassay_007114_16	euxassay_007114_17	euxassay_007114_18	euxassay_007114_19

euxassay_007114_20	euxassay_007114_21	euxassay_007114_22	euxassay_007114_23	euxassay_007114_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11765_wholemount_strong.wlz

11765_wholemount_moderate.wlz

11765_wholemount_weak.wlz

11765_wholemount_possible.wlz

11765_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11765_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9963
Entity Detected:	Gh, growth hormone ( MGI:95707)
Sequence:	sense strand is shown >T9963 AGCATCCTAGAGTCCAGATTCCAAACTGCTCAGAGTCCTGTGGACAGATCACTGCTTGGCAATGGCTACA GACTCTCGGACCTCCTGGCTCCTGACCGTCAGCCTGCTCTGCCTGCTCTGGCCTCAGGAGGCTAGTGCTT TTCCCGCCATGCCCTTGTCCAGTCTGTTTTCTAATGCTGTGCTCCGAGCCCAGCACCTGCACCAGCTGGC TGCTGACACCTACAAAGAGTTCGAGCGTGCCTACATTCCCGAGGGACAGCGCTATTCCATTCAGAATGCC CAGGCTGCTTTCTGCTTCTCAGAGACCATCCCGGCCCCCACAGGCAAGGAGGAGGCCCAGCAGAGAACCG ACATGGAATTGCTTCGCTTCTCGCTGCTGCTCATCCAGTCATGGCTGGGGCCCGTGCAGTTCCTCAGCAG GATTTTCACCAACAGCCTGATGTTCGGCACCTCGGACCGTGTCTATGAGAAACTGAAGGACCTGGAAGAG GGCATCCAGGCTCTGATGCAGGAGCTGGAAGATGGCAGCCCCCGTGTTGGGCAGATCCTCAAGCAAACCT ATGACAAGTTTGACGCCAACATGCGCAGCGACGACGCGCTGCTCAAAAACTATGGGCTGCTCTCCTGCTT CAAGAAGGACCTGCACAAAGCGGAGACCTACCTGCGGGTCATGAAGTGTCGCCGCTTTGTGGAAAGCAGC TGTGCCTTCTAGCCACTCACCAGTGTCTCTGCTGCACTCTCCTGTGCCTCCCTGCCCCCTGGCAACTGCC ACCCCTGCGCTTTGTCCTAATAAAATTAAGATGCATCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30250975 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30250975 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11762 same embryo
	EMAGE:11764 same embryo
	EMAGE:11766 same embryo
	EMAGE:11761 same embryo
	EMAGE:11763 same embryo
	EurExpress:euxassay_007114 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source