EMAGE mouse gene expression spatial database

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EMAGE:11786

Gabarapl1 gamma-aminobutyric acid (GABA) A receptor-associated protein-like 1 ( MGI:1914980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007032. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007032_01	euxassay_007032_02	euxassay_007032_03	euxassay_007032_04

euxassay_007032_05	euxassay_007032_06	euxassay_007032_07	euxassay_007032_08	euxassay_007032_09

euxassay_007032_10	euxassay_007032_11	euxassay_007032_12	euxassay_007032_13	euxassay_007032_14

euxassay_007032_15	euxassay_007032_16	euxassay_007032_17	euxassay_007032_18	euxassay_007032_19

euxassay_007032_20	euxassay_007032_21	euxassay_007032_22	euxassay_007032_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11786_wholemount_strong.wlz

11786_wholemount_moderate.wlz

11786_wholemount_weak.wlz

11786_wholemount_possible.wlz

11786_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11786_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 09 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 08 18

trigeminal v nerve

strong

regional

strong expression: see section 10 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 11 16

cervical ganglion

moderate

regional

moderate expression: see section 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

neural retina

strong

regional

strong expression: see section 02 03 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 09 20

urinary system mesentery

strong

regional

strong expression: see section 04 05 06 07 08 09 21 22 23

kidney calyx

strong

regional

strong expression: see section 21 moderate expression: see section 09 10 11 17 18 19 20

kidney pelvis

moderate

regional

moderate expression: see section 19

axial skeleton

moderate

regional

moderate expression: see section 11 12 13 14 15

axial skeleton tail region

strong

regional

strong expression: see section 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6829
Entity Detected:	Gabarapl1, gamma-aminobutyric acid (GABA) A receptor-associated protein-like 1 ( MGI:1914980)
Sequence:	sense strand is shown >T6829 GTTTTTGTGTTTCCAGCTCTTGGGAAAAGCCACCCTATTTCTTTACCTGGTGGTCCTTTACCTTCAGGCC GCGAGATCTCCAGGTCCTTCTCTGGACGTTTAGCCACGTATGCGGCGACTGGCGCGTGGGAGCTGACGCG GGTGGAGCGATTGTGATAAGGCTGACATTGACTGGGGGTGGCCAGACGCGAGACGCAGTCATCAGCGCGC GCAGCAGGCCCCGTCCGGGGAGCCCCTACATCCTGAGGTCCATCATGAAGTTCCAGTATAAGGAGGACCA CCCCTTCGAGTATCGGAAAAAGGAAGGTGAAAAGATTAGGAAGAAATACCCGGACCGGGTGCCGGTCATC GTGGAGAAGGCTCCTAAAGCCAGGGTCCCTGATCTGGATAAGAGGAAGCACCTTGTGCCCTCCGACCTCA CTGTTGGCCAGTTCTACTTCTTAATCCGGAAGAGGATCCACCTGAGACCTGAGGACGCCTTATTCTTCTT TGTCAACAACACCATCCCTCCCACCAGTGCTACCATGGGCCAGCTGTATGAGGACAACCACGAGGAAGAC TATTTTCTGTATGTGGCCTACAGTGATGAAAGTGTCTATGGAAAATGAGGCAGAAGCCCAGCAGATGGGA GCACCTGGACTTGGGGGTAGGGGAGGGGTGCGCGTGGGACTTGGGGAACCAGAGGGAGGGCTCCCAACCA TGGAGGAGACGCAAGGTGAAGACATCGGGAAACATCACACCGCACACGCTGTCGTCTTTTTTTCACAAGC TCAATTGTTACTTTTCTCTGCTTCCTCAGCCCAGGAAGAACTTGTGTTGCATTGGCTGTGAGAGCGGGAT GGGGACGACATGAATCACAGCTTTGCTGTGAGCTTTCTCTCGTGCAGTTTCATCAGAGGTTTGGGAGGAG GGCAGGTGGCCGCTAGAGAAGAATACAATTTAGCAGCAACTGCCGGCTCTTTGGGCAGAGGTTTGATTTT TGGCATTTGTACAAGCCGCACGGGCAAAGGGGGAAAAGAATGCCGTAGGGGACCACACCTAAGGGACCAA AAGATACCCACTGTCACTGAAGCATTGTACCCCTAACCTCTGTCTCCATACCTTCCTCTCCTAGTCCTTT TCTTCCACCCAGGCTTCATAGCTCCGCCCCAGTTGTGGCAGGAGACATTCGAACAGACGTTTAGACGTTT AGAGTCCTCTTGGAAAATAGGGCCGTGCGGGAACGGTTATGATTGCCAGCGTTTTGGATGGCATTAAACT GGAGTTCAGGGTTGAACATGTCCTGCTCCTCATCCCATAAGTACCTTGGACTTTCAGGAGCCTCAGGATC AGTCCTCTAGCATTTCCTTCACCTTTCTCATGTACAGGGGCAGCGTGTCTGTGTGTGGAGGGTCCGGGTT ACAAGGTAAGCTAGGTTGCCCACTTGCCCACTCGTCCTCTCCCTGCTGCAGTCTCTTGGGTCTCACACGC ACTGCAAATGCTAAGCGATGGCCAGCTGTCTCCCTCCGTGGCATCTGCTATTCAAAGAAGATGGAGTGAG AGGGGCGCCAGAGTTTCATGGGGGCTTTACTCATTCATTTCATTTAGGGACTCTGTGGGGGGAGGGGCAG GCATTGCATTCTCACATGACATTTCAATTCATCTCTACCAATGAAGAGCGTCCTCCCTGTTGGGAGCAAT CTGTACGTCTGTTTTGTCAGCTTCAGGTTCTGGTACTTTGAAGTCAATGCTGTCAGGCCAGGGAAATAAA ATAATTGCTTACCTTAAAAGTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3711973 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3711973 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11791 same embryo
	EMAGE:11790 same embryo
	EMAGE:11788 same embryo
	EMAGE:11787 same embryo
	EMAGE:11789 same embryo
	EurExpress:euxassay_007032 same experiment
	MGI:4824960 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source