EMAGE mouse gene expression spatial database

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EMAGE:11787

Gpr173 G-protein coupled receptor 173 ( MGI:1918021)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007034. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007034_01	euxassay_007034_02	euxassay_007034_03	euxassay_007034_04

euxassay_007034_05	euxassay_007034_06	euxassay_007034_07	euxassay_007034_08	euxassay_007034_09

euxassay_007034_10	euxassay_007034_11	euxassay_007034_12	euxassay_007034_13	euxassay_007034_14

euxassay_007034_15	euxassay_007034_16	euxassay_007034_17	euxassay_007034_18	euxassay_007034_19

euxassay_007034_20	euxassay_007034_21	euxassay_007034_22	euxassay_007034_23	euxassay_007034_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11787_wholemount_strong.wlz

11787_wholemount_moderate.wlz

11787_wholemount_weak.wlz

11787_wholemount_possible.wlz

11787_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11787_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 10 11 18

spinal cord

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 12 15 16

cervical ganglion

strong

regional

strong expression: see section 09 17

thoracic ganglion

strong

regional

strong expression: see section 11 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

neural retina

strong

regional

strong expression: see section 01 02 03 04 05 24

tongue

strong

spotted

strong expression: see section 13

stomach

strong

spotted

strong expression: see section 05 06 07 08 09 10 11 moderate expression: see section 02 03 04

midgut

strong

spotted

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8400
Entity Detected:	Gpr173, G-protein coupled receptor 173 ( MGI:1918021)
Sequence:	sense strand is shown >T8400 ACCCTCATCTCTCTGTCCCTCTGTCTGTATCTCCCTCCATTGTTCCCATTTGCAGGTGTAGCTCAGCAAG AAAACTGATGGAGTCCGCCAGGGCCCATCAAATACTGAACTGGCCAATCCCCTAGGGTTGGGGGTAGCCT TTGCCCCTCAGTATGGCCAACACCACCGGAGAGCCCGAAGAGGTGAGCGGCGCACTGTCTCTGCCATCAG CATCGGCTTATGTGAAGCTGGTGCTGCTGGGACTGATCATGTGTGTAAGCCTGGCAGGCAATGCCATCTT GTCCCTGCTGGTGCTCAAGGAGCGTGCCCTGCACAAGGCTCCTTACTACTTTCTGCTGGACCTGTGCCTA GCCGATGGCATACGCTCTGCCATCTGCTTCCCCTTTGTACTGGCTTCTGTGCGCCATGGCTCCTCATGGA CCTTCAGTGCACTCAGCTGTAAGATTGTGGCCTTTATGGCTGTGCTCTTTTGCTTCCATGCGGCCTTCAT GCTGTTCTGCATCAGCGTCACCCGCTACATGGCCATCGCCCACCACCGCTTCTATGCCAAGCGCATGACA CTCTGGACATGCGCAGCTGTCATCTGCATGGCCTGGACCTTGTCTGTGGCCATGGCTTTCCCACCTGTCT TTGATGTGGGCACCTACAAGTTTATCCGAGAGGAGGACCAGTGCATCTTTGAGCATCGCTACTTCAAAGC AAATGACACACTGGGCTTTATGCTTATGTTGGCTGTGCTCATGGCAGCCACACATGCTGTCTATGGCAAG CTGCTACTCTTCGAGTATCGTCACCGCAAGATGAAGCCAGTGCAGATGGTGCCAGCCATCAGCCAAAACT GGACATTCCATGGCCCTGGGGCTACCGGCCAGGCTGCTGCCAACTGGATCGCTGGTTTTGGCCGTGGGCC CATGCCACCAACTCTGCTGGGTATCCGGCAGAATGGGCATGCAGCTAGCCGGCGGCTACTGGGCATGGAC GAGGTCAAGGGTGAAAAGCAGCTGGGCCGAATGTTCTACGCGATTACACTGCTCTTCCTGCTCCTCTGGT CACCATACATTGTGGCCTGCTACTGGCGAGTGTTTGTGAAAGCCTGCGCTGTGCCCCACCGCTACTTGGC CACTGCTGTTTGGATGAGCTTCGCCCAGGCTGCCGTCAACCCAATCGTCTGCTTCCTGCTTAACAAGGAC CTCAAGAAGTGCCTGAGGACTCATGCCCCTTGCTGGGGCACAGGAGGTGCCCCAGCTCCCAGAGAACCCT ACTGTGTCATGTGAAGCAAGCCCGTAGGTGATAGGCAGGGGGAAAGTCAGAATCACCATACGGGGGCCAG CAGCAGGGGAAGGGTGGGACCCATACAGAAGCCTTCTCTTTCAAGCTTCAGTCCAGGCTCTCAAACCATC CATAACTGAGGCAACTCGCCAACACCTCCCTAGGGTTGGAAACGGGGTGAGGAACTGGAAAAGAGGCATT TCTAGTTTTGTGGGGATTGTCCCGCAGCCTCCTCCACTTCAACAATCTCCTTTTCCCATGCCCTCCCTGC CTTCCCTTCCCTCTCCCCTCTCCTATCTTTCTAAAGTAATGATTCAGTAAATGAAAACAAACTGAAAATG CAGGGTTTTTAATAATAACAGCTGAGGAAGTAGGCTCTCTTGCTTAAATGTCCCCCCTTTTGACATTGTC AAGAAATGGGAAGTTTCTCCTGTAAAATAGATTCCCAGAGATCGTATCGTCCCCTGGGCTCCCATTCACC CTCCCCTTCCAAATCCTTTAGCAAGACTTGGATGTTTAGGAAATATTGGCTTAAGTCTTCAGACAAGAGG GACCAAATGGGGTGCTATATTCCTTGGGAGCAAGGATGTTTAAATTGCTATGTGCTATGTGCAATGTTGT TTTTGGCTAACCTTGGTCCAAAGCCCAATCTCCAATCCCTCCCTACCATGTCCAGACTTCACAGGTGTTT CTTGAGGCTGTTTGAGAAGCCAAGAGCATACAAAGAAGAGCCTCCATGATGTGCCCAGGCTAGATGAAGA GCAGAATGGGGGATGGGCTCCTCAACCAGAATATACCAAACTTAGCTACGTTCACTCAAGCTTCCTCCAG GAATATATAACTAACTTTTCCTCCTAGGAATATATATCCACTTTAGTCCCCATTAGGGATGATGTGGGTA TAAGCTGACAGGGAAAGTCACCCATGGGGAAATAGTTGAACTCTCCAGAGTACCTAGAGCCCAGACCATG GGGTCAGAGCTAAATTCAACCCCACAATTATCCCTTGGGTTCACAATACTCCCCCCTAAAATAAAATTCT TTGGAGACCTTGATAATCAAAAGTTATACAAACTTCAAGGCACTGTGGAATTGAGTCCATTTCTGCTTGA CCTCTTTTTGTCCCTTCCAAGCCTCACAGTCCTTGAGCTCCTCCTTGGTTGGGCCCCAAACATCCTTCCC AGAAGTTTTTTCTCCATCCACTCTTACAGGTGGATGGTCCCAAGCCTCTTTTCCATGGACAAAAACCACT TTGGGCTTACTTCAAGGCCAGTTCTACAGACTGGTGAGCCAAGTTGGAACTCTTTGCCTAAGTCTGGGGC AGTGATCTCTCCATCTCCTTTCCTTCCTTATAAACTCCTGTCCTAAACTCCACCTCTACCCCAAGATAGG CCCTGAACTGAGGTAGTCTTGACCCAAAAGCAATGAAGGGGGCACTCTAGGCAGAAGAGATATTGATGAC CCTGGTTGTGGAGTGGCCCTGGAAAAGCAAGCCAGAGGAGCTGAGAGGCTGGGAATGTGTGTTTGGGGTG GGGCTATTCTCTCAGTGATGATGATAGAAATGCCTTTCTGAGGGCTGGGGTGCAAAGACCAAGGTGGGAG GGCAAATAGGGTAAACTCGGGAGGGTTTTCCTCCGTTTCCTTTTTCTAACTGCCTGGATTCATCATTGGA TTTTGTAAATGGAAAAAGTGAAATGAACAATGTTATATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686241 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686241 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11791 same embryo
	EMAGE:11790 same embryo
	EMAGE:11786 same embryo
	EMAGE:11788 same embryo
	EMAGE:11789 same embryo
	EurExpress:euxassay_007034 same experiment
	MGI:4825181 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source