EMAGE mouse gene expression spatial database

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EMAGE:11793

Arc activity regulated cytoskeletal-associated protein ( MGI:88067)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007072_01	euxassay_007072_02	euxassay_007072_03	euxassay_007072_04

euxassay_007072_05	euxassay_007072_06	euxassay_007072_07	euxassay_007072_08	euxassay_007072_09

euxassay_007072_10	euxassay_007072_11	euxassay_007072_12	euxassay_007072_13	euxassay_007072_14

euxassay_007072_15	euxassay_007072_16	euxassay_007072_17	euxassay_007072_18	euxassay_007072_19

euxassay_007072_20	euxassay_007072_21	euxassay_007072_22	euxassay_007072_23	euxassay_007072_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11793_wholemount_strong.wlz

11793_wholemount_moderate.wlz

11793_wholemount_weak.wlz

11793_wholemount_possible.wlz

11793_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11793_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6182
Entity Detected:	Arc, activity regulated cytoskeletal-associated protein ( MGI:88067)
Sequence:	sense strand is shown >T6182 GCGAGTTCTCCCGCAGCCGCAGTCTCTGGGCCTCTCTAGCTTCAGCGGCGACGAGCCTGCCACACTCGCT AAGCTCCTCCGGCACCGCACACCTGCCACTGCCGCTGCAGCCGCCGGCTCTGCTCCCTTCCGGCTTCTGC CTCAGAGGAGTTCTTAGCCTGTTCGGAGCCGCAGCACCGACGACCAGATGGAGCTGGACCATATGACCAC CGGCGGCCTCCACGCCTACCCTGCCCCGCGGGGTGGGCCGGCCGCCAAACCCAATGTGATCCTGCAGATT GGTAAGTGCCGAGCTGAGATGCTGGAACACGTACGGAGGACCCACCGGCATCTGTTGACCGAAGTGTCCA AGCAGGTGGAGCGAGAGCTGAAAGGGTTGCACAGGTCGGTGGGCAAGCTGGAGAACAACTTGGACGGCTA CGTGCCCACCGGCGACTCACAGCGCTGGAAGAAGTCCATCAAGGCCTGTCTTTGCCGCTGCCAGGAGACC ATCGCCAACCTGGAGCGCTGGGTCAAGCGTGAGATGCACGTGTGGAGGGAGGTCTTCTACCGTCTGGAGA GGTGGGCTGACCGCCTGGAGTCCATGGGCGGCAAATACCCAGTGGGCAGCGAGCCGGCCCGCCACACTGT CTCTGTAGGTGTGGGGGGTCCAGAGCCCTACTGCCAGGAAGCTGATGGCTATGACTATACCGTTAGCCCC TATGCCATCACCCCGCCACCTGCCGCAGGAGAACTGCCTGAACAGGAGTCAGTTGAGGCTCAGCAATATC AGTCTTGGGGGCCAGGTGAGGATGGGCAACCGAGCCCTGGTGTGGATACACAGATCTTCGAGGACCCACG GGAGTTCCTGAGCCACCTGGAAGAGTACCTGCGGCAGGTGGGTGGCTCTGAAGAATATTGGCTGTCCCAG ATCCAGAACCACATGAATGGGCCAGCCAAGAAGTGGTGGGAGTTCAAGCAGGGCTCGGTGAAGAACTGGG TGGAGTTCAAGAAGGAGTTTCTGCAATACAGTGAGGGTACACTCTCCCGTGAAGCCATTCAGCGGGAGCT GGAGCTGCCGCAGAAGCAGGGTGAACCACTCGACCAGTTCCTCTGGCGCAAGCGGGACCTGTACCAGACA CTGTATGTGGACGCTGAGGAGGAGGAGATCATTCAGTATGTGGTGGGCACCCTGCAGCCCAAACTCAAGC GCTTTCTGCGCCACCCACTCCCCAAGACCCTGGAGCAGCTTATCCAGAGGGGTATGGAAGTGCAGGACGG CCTGGAGCAGGCAGCTGAGCCTTCTGGCACCCCACTGCCCACAGAGGATGAGACGGAGGCACTCACACCT GCTCTTACCAGCGAGTCAGTAGCCAGTGACAGGACCCAGCCTGAATAGAGGGGCCAGCCCAGGGTCCCCA GCCCGCCTGCCACACCCAGCCTGTGGCTTTTGCCAACTAGGACTTGAGCTGGGGCTGACTCCCAAAGGGA TGCCCTGTCCAGCCAGACATCTACTCACCCACTGACCTGGCCTGACTCACAACTGCCACACAACCATGCT ACATGGACTGAACATCAAGAAGCCCCTTTCCCATAGGGCTCCCACCTGCCGCCTACCCCTCATCTGTCTG CCCTGGTCCTGGCCCCCAGCCCCATTGGCCTCACCCTCTACACTCTCAGACCATCACAGAACACGATCTG GCTTCCTCATTCTGCTCCAGTGTCCAGGGCTCTTTGGGTAATCAAGAAACCAAGTGTCTGAAAGGCAACA AAAAGTAGGCACCAAAACCCAGGGGACATCCTAGGGCAGATGCTAAAGCAGAGATGCTGAGGGAACCTCA ACTTCCGGGGATGCAGCCCACTCCTGCAGACACAGCAGATCCAGCTGGTACCCTAGCTGCCGCCCAGGGC AACCGGCCAGTCTTGGGCAGCATAGCTCCCCTCCCAGGGGTGAGCTGAAGCCACAAATGCAGCTGAAGCA GCAGACCTGACATCCTGGCACCTCCTGGCCCCCAGTGATTCATACCAGTGAAGAAGAGCAGAGCTCAGCT CCGTGACTCAGCCATGGCAGGCGGAGGGTCCCCGAGGGGCTGAGTCCTCACACCCAGCCGAGGCAGCGGC TGGAGCCTACAGAGCCAGGAGAATGACACCAGGTCTCAAGGCTGCTGAGAAGTCTTTGCTGCCATGTCTG GAAAGGGCATCACCACACCACCAGCACCAGCACCATCCTCTCTTCTCCTGAAGCTGCCTACATGGGTTCC AAGACACTTTCAAGGCAGAGAAAACAAGATTACAGAGAGGAGGTGCCTGGCAGGGGGCAGCACCCCAGCT CAGCCCAAGAGCTGAAGGTGAAGACAAGCCAGCATGAAACCACGGGTCTGCCATGATGCCCGCCCCGCTG GCCGCTCACTAGCTGCCTGCCATTAGCCTCTGCAGCTTGAGCAGGGTCTGTACCCTTGGTCCTCTTGGCA CAGAGCCCAGCTCGCTGCATGGCCTTTGGCTCCCCGACCAGACCCTTGCAGGAGCCTTAAGGCTTGGGCC CCTGCCCAGCCTGATCTTTCCTGCTGTGCCCTGCCTGCCAGGTCAAGCCCAGTCCCAGGAGACCCCAGGC CTTGGCTCCTAGGCTGTTCCATGAACCTCCCTGACCTGCCTGGTGATTGCCCAGCTGAAACCTCAGCCAA GCCCCAGCTCCAATTACCTTGTGCTGGTAGCTGCTTGTGTCTGCAGTCTGAGTAGGCCTTGTTGCGGTTC CTCCATCTGCCTGGTCTATTGGTGTTCTGAGACCAATTCCACTGATGTTCTGACAGATCCTCCACCCTGT GCCCCTGCCAGCCCCCACAAGTTTATTTTTGCACAAAAGCCATGACCCATACTCATTTGGCTGGCATAGG GTGTGTAGGTAGGCCCTGGGGACTAGGGAGACCCTGGAGATCTCAAGAGAGTGTGGCTATCCCCTATTTT CACCAAGCCTTGAATATCCAGCCAGGCTGTCTGCCCATACCATCTTACCTCAAAGACAGATATATATCTA TATATGATTTTGTTAATAAAACTATGAAACTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3498057 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3498057 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11797 same embryo
	EMAGE:11798 same embryo
	EMAGE:11792 same embryo
	EMAGE:11795 same embryo
	EMAGE:11796 same embryo
	EMAGE:11794 same embryo
	EurExpress:euxassay_007072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source