EMAGE mouse gene expression spatial database

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EMAGE:11796

Ncl nucleolin ( MGI:97286)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007121_01	euxassay_007121_02	euxassay_007121_03	euxassay_007121_04	euxassay_007121_05

euxassay_007121_06	euxassay_007121_07	euxassay_007121_08	euxassay_007121_09	euxassay_007121_10

euxassay_007121_11	euxassay_007121_12	euxassay_007121_13	euxassay_007121_14	euxassay_007121_15

euxassay_007121_16	euxassay_007121_17	euxassay_007121_18	euxassay_007121_19	euxassay_007121_20

euxassay_007121_21	euxassay_007121_22	euxassay_007121_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11796_wholemount_strong.wlz

11796_wholemount_moderate.wlz

11796_wholemount_weak.wlz

11796_wholemount_possible.wlz

11796_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11796_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6137
Entity Detected:	Ncl, nucleolin ( MGI:97286)
Sequence:	sense strand is shown >T6137 GCTCTTCCGAGCTGCTCGCTCTCCACACGCGCCGCCGCCGTAATCCGCCACCATGGTGAAGCTCGCAAAG GCTGGCAAAACCCACGGTGAGGCCAAGAAAATGGCTCCTCCTCCAAAGGAGGTGGAAGAGGATAGTGAAG ATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGTCATCCCTCAGAAAAAAGG CAAAAAGGCTACCACAACCCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAAAAGGCTGCAGTTCCCACA CCAGCTAAGAAAGCAGCTGTGACCCCAGGCAAAAAGGCAGTAGCCACACCAGCTAAGAAAAACATTACAC CAGCCAAAGTCATTCCAACACCGGGTAAGAAGGGAGCTGCACAAGCAAAAGCGTTGGTACCAACTCCTGG TAAAAAGGGAGCTGCCACTCCAGCTAAGGGGGCTAAGAACGGTAAGAATGCCAAGAAGGAAGACAGTGAT GAGGATGAAGATGAAGAGGATGAAGAAGATAGCGATGAGGATGAAGATGATGAGGAAGAGGATGAGTTTG AGCCACCAATAGTAAAAGGAGTGAAGCCAGCAAAAGCAGCTCCTGCTGCTCCTGCCTCCGAGGATGAGGA AGATGATGAGGATGAAGATGATGAGGAAGAGGAAGATGAAGAAGAGGAAGATGACTCTGAGGAAGAAGTT ATGGAGATCACAACAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTATGAAAGCCAAGAGTGTGG CTGAGGAGGAGGATGAGGAGGAAGAGGATGAAGATGACGAGGATGAGGATGATGAGGAAGAGGATGACGA AGATGATGATGAGGAAGAAGAGGAGGAAGAACCTGTTAAAGCAGCACCTGGAAAACGGAAGAAGGAGATG ACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAGTAGAAGGCTCAGAACCAACTACACCTTTCA ATCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTAATGAATTAAAATTTGCCATCAGTGAACTTTT TGCTAAAAATGATCTTGCTGTTGTGGATGTCAGAACTGGTACAAATAGGAAATTTGGTTATGTGGACTTT GAGTCTGCTGAAGACCTAGAAAAGGCCTTGGAGCTCACTGGTTTAAAAGTGTTTGGCAATGAAATTAAAC TAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGCAAGAACACTTCTAGCCAAAAACCTCTC TTTCAACATCACTGAGGATGAATTAAAGGAAGTGTTTGAAGACGCCATGGAGATCAGATTAGTCAGCCAG GATGGGAAAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTGAAGCTGATGCAGAGAAAAATTTGGAAG AAAAGCAGGGGGCAGAAATTGATGGACGATCTGTTTCACTCTACTATACTGGAGAGAAAGGTCAAAGGCA AGAGAGAACTGGAAAGACCAGCACTTGGAGTGGTGAATCAAAGACTTTGGTTTTAAGTAACCTTTCCTAC AGTGCAACAAAAGAAACTCTTGAGGAAGTATTTGAGAAAGCAACTTTTATCAAAGTGCCCCAGAACCCAC ATGGCAAACCTAAAGGGTATGCATTTATAGAATTTGCTTCATTTGAAGATGCTAAAGAAGCTTTAAATTC CTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAGTTGCAAGGATCCAATTCGAGAAGTCAA CCATCCAAAACTCTGTTTGTCAAAGGTCTGTCTGAGGATACCACTGAAGAGACCTTAAAAGAATCATTTG AGGGCTCTGTTCGTGCAAGAATAGTCACTGATCGGGAAACTGGTTCTTCCAAAGGGTTTGGTTTTGTAGA CTTTAATAGTGAGGAAGATGCCAAAGCTGCCAAGGAGGCCATGGAAGATGGAGAAATTGACGGAAACAAA GTTACCTTGGACTGGGCCAAACCTAAGGGTGAAGGTGGCTTTGGTGGTCGAGGTGGAGGCAGAGGAGGTT TCGGAGGCAGAGGTGGAGGCAGAGGTGGAAGAGGTGGATTTGGAGGAAGAGGCCGGGGAGGCTTTGGAGG TAGAGGAGGCTTCCGAGGCGGCAGAGGAGGAGGGGGAGACTTCAAGCCACAAGGAAAGAAGACGAAGTTT GAATAGTTCCTTCCATCCCATTCTTTCCCTCTTCATTTTAAAGAAAGGACTCTGGGGTTTTTACTCTGTT ACCTGTTCAATGACAGAGCCTTCTAAGGACATTCCAAGACAGTAAAGATCCTAAACTCTGTTGCCAGACT TAATACTTCCTAGAGGTTACATTTGGATTGTTGCTTAAGAGCTTGGCAAGACCAACTCTGCCCAGTGGGA ATTTCAGTTGTGTTCCTACTGCTTTCAAGAATTCCCAGCTACAAATGTGGAAATTGGTGGGGAACAGCTT CATCATGATTCTGATCTGGAAGATTTAATTTGTATCTTAGAAGGGTGTGTACTGGAAAAGTGGTTGATAG AGCACATGGATCAGTGCTGGAAATACCATACAAGAGCTTGTGTGGTATGTAATTCTATTCCCAGCATAAT AAGAAAAAACAGTACAGTGCAATCTGAAATGACCTGGACAAGGAATGGTACCCCATTGGGTTGAAAAATT CACATAGTCAGGATGGTTGCACATGCCTTTAACCCCAGCACTTGGGAGGGAGAGGCTGGTAGATCTGAGT TTGATCTGATGTACAGAGAGTTCCAGAACAGCCAGGGCTGTTTATTAGAGAAAAAACCCTGTCTTGAAAA AACAAGAAAAAAAAACCTCACAGACAACCAAATACAGAGTTTTAGCCACTGAGTACCAAGTCTCAGCAGC AAGCTGGGAGTGACGTAATCCAATCCCATGGGAGGCTAAAGCAGTCTATGACTTCAATGCCAGTTGGGTC TGCAGAACTGAGTTGCTGGATAGTGAGGGATACATAGAAACTTTGTCTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3495665 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3495665 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11793 same embryo
	EMAGE:11797 same embryo
	EMAGE:11798 same embryo
	EMAGE:11792 same embryo
	EMAGE:11795 same embryo
	EMAGE:11794 same embryo
	EurExpress:euxassay_007121 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source