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EMAGE:11927

Krt12 keratin 12 ( MGI:96687)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927
"Pseudo-wholemount" of euxassay_011029. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011029_01 euxassay_011029_02 euxassay_011029_03 euxassay_011029_04
EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927
euxassay_011029_05 euxassay_011029_06 euxassay_011029_07 euxassay_011029_08 euxassay_011029_09
EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927
euxassay_011029_10 euxassay_011029_11 euxassay_011029_12 euxassay_011029_13 euxassay_011029_14
EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927 EMAGE:11927
euxassay_011029_15 euxassay_011029_16 euxassay_011029_17 euxassay_011029_18 euxassay_011029_19
EMAGE:11927 EMAGE:11927 EMAGE:11927
euxassay_011029_20 euxassay_011029_21 euxassay_011029_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11927Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11927_wholemount_strong.wlz
11927_wholemount_moderate.wlz
11927_wholemount_weak.wlz
11927_wholemount_possible.wlz
11927_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11927_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 weak expression: see section 07 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36566
Entity Detected:Krt12, keratin 12 ( MGI:96687)
Sequence:sense strand is shown

>T36566
GAAGAAGAACCACGAAGAGGAACTCCAAAGTTTCCAGGCAGGTGGTCCAGGTGAGGTCAATGTAGAAATG
GACGCGGCCCCTGGAGTGGATCTCACCAAGGTCCTCAATGAAATGAGAGCCCAGTATGAAGCCATGGCTG
AGCAAAATCGGAAAGATGCAGAGGCCTGGTTCCTTGAAAAGAGCGGAGAGCTCCGAAAAGAGATCAGCAG
CAACACGGAGCAGCTTCAGTCCAGCAAGAGCGAGGTCACCGACCTGAAGCGCATGGTGCAGAACCTAGAG
ATTGAACTCCAGTCCCAGCTCGCCATGAAGTCGTCCCTGGAAGGCTCGCTGGCTGAAACCGAGGGTGGGT
ACTGCTGCCAACTGTCTCAGGTGCAGCAGCTGATCGGCAGCCTGGAGGAGCAACTCCAACAGGTGCGGGC
TGACGCGGAGCGCCAGAATGCGGACCACCAGCGGCTGCTGGGCGTCAAGGCTCGCCTGGAGATGGAGATT
GAGACCTACCGCCGGCTGCTGGAGGGGGACAGCCAAGGTGACGGTTTTGACGAGAGCTCATCCCTTTCCG
TCTCCAAACCGCAGACACCATCAGTCGATTCCTCTAAAGACCCAAATAAAACTCGAAAAATCAAGACAGT
CGTGCAGGAGAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90151. Forward Primer - name:090151_F_cDNA_Krt1-12, sequence:GAAGAAGAACCACGAAGAGGAA; Reverse Primer - name:090151_N_SP6_cDNA_Krt1-12, sequence:ATCTCCTGCACGACTGTCTTGAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11925 same embryo
 EMAGE:11928 same embryo
 EMAGE:11924 same embryo
 EMAGE:11923 same embryo
 EMAGE:11926 same embryo
 EurExpress:euxassay_011029 same experiment
 MGI:4825825 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS