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EMAGE:11953

Map3k5 mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953
"Pseudo-wholemount" of euxassay_011097. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011097_01 euxassay_011097_02 euxassay_011097_03 euxassay_011097_04
EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953
euxassay_011097_05 euxassay_011097_06 euxassay_011097_07 euxassay_011097_08 euxassay_011097_09
EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953
euxassay_011097_10 euxassay_011097_11 euxassay_011097_12 euxassay_011097_13 euxassay_011097_14
EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953 EMAGE:11953
euxassay_011097_15 euxassay_011097_16 euxassay_011097_17 euxassay_011097_18 euxassay_011097_19
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euxassay_011097_20 euxassay_011097_21 euxassay_011097_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11953Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11953_wholemount_strong.wlz
11953_wholemount_moderate.wlz
11953_wholemount_weak.wlz
11953_wholemount_possible.wlz
11953_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11953_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 09 10 11 12 13
thyroid gland
weak weak
regionalweak expression: see section 09 13 14
vibrissa
weak weak
regionalweak expression: see section 04 05 06 20 21 22
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 07 14 weak expression: see section 15
trigeminal v ganglion
strong strong
single cellstrong expression: see section 06 07 16 moderate expression: see section 04 05 17 18 19 20 21 weak expression: see section 03
lens
weak weak
regionalweak expression: see section 01 02
naris
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12 15
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 15 weak expression: see section 07 08 09 10 12 13 16 17 18
bladder
weak weak
regionalweak expression: see section 11 12 13
clavicle
weak weak
regionalweak expression: see section 07 08 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36616
Entity Detected:Map3k5, mitogen-activated protein kinase kinase kinase 5 ( MGI:1346876)
Sequence:sense strand is shown

>T36616
GAGAAAGGATAGTGAGAGGCGAGCTACCCTTCACAGAATCCTGACCGAAGACCAGGACAAGGTTGTAAGG
AACCTAATGGAATCTCTGGCCCAGGGTGCTGAAGAGCCTAAACTAAACTGGGAACACATCACAACCCTCA
TCTCAAGCCTCAGAGAGTTTGTGAGGTCCACTGACCGAAAAATCATAGCCACTACATTGTCCAAGCTCAA
GCTGGAGCTGGACTTCGACAGCCACGGCATCAGCCAGGTGCAGGTGGTCCTCTTTGGCTTCCAGGATGCG
GTCAATAAAGTTCTTCGGAATCATAACATCAAGCCACACTGGATGTTTGCCCTGGACAGCATCATCCGAA
AGGCTGTGCAGACTGCAATTACCATTCTGGTTCCAGAGTTGAGGCCACATTTTAGCCTTGCATCAGAGAG
CGACACAGCTGATCCGGAAGACCTGGATGTCGAAGATGAACACGAGGAATTGTCTTCAAATCAGACAGTC
CGACGGCCTCAGGCATCACTGACGGACGCTGTGGCTACCTCGGGGGTGAGCACGCTAAGCTCCACTGTGT
CCCATGACTCCCAGAATGCACACCGGTCGCTGAACGTGCAGCTTGGAAGGATGAAGATTGAAACTAATAG
GTTACTTGAAGAGCTGGTTCGGAAAGAGAGAGAGTTACACGGCTCTCCATCAGGCATTGAGGAAAAAGAC
CAAGAAATTAGGCACCTGAAGCTCAAGTCCCAGCCCATAGATATCCCTGGGTTTCCTGTGTGCCACCTGA
ACTCTCCTGGCACGACCACGGAAGATTCTGAACTTCCTGGCTGGCTGAGAGAAAATGGAGCTGATGAAGA
CAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 98094. Forward Primer - name:098094_F_cDNA_Map3k5, sequence:GAGAAAGGATAGTGAGAGGCGA; Reverse Primer - name:098094_N_SP6_cDNA_Map3k5, sequence:GTGTCTTCATCAGCTCCATTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11951 same embryo
 EMAGE:11954 same embryo
 EMAGE:11950 same embryo
 EMAGE:11952 same embryo
 EurExpress:euxassay_011097 same experiment
 MGI:4826082 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS