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EMAGE:12034

Csrp2bp cysteine and glycine-rich protein 2 binding protein ( MGI:1917264)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034
"Pseudo-wholemount" of euxassay_008407. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008407_01 euxassay_008407_02 euxassay_008407_03 euxassay_008407_04
EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034
euxassay_008407_05 euxassay_008407_06 euxassay_008407_07 euxassay_008407_08 euxassay_008407_09
EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034
euxassay_008407_10 euxassay_008407_11 euxassay_008407_12 euxassay_008407_13 euxassay_008407_14
EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034
euxassay_008407_15 euxassay_008407_16 euxassay_008407_17 euxassay_008407_18 euxassay_008407_19
EMAGE:12034 EMAGE:12034 EMAGE:12034 EMAGE:12034
euxassay_008407_20 euxassay_008407_21 euxassay_008407_22 euxassay_008407_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12034Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12034_wholemount_strong.wlz
12034_wholemount_moderate.wlz
12034_wholemount_weak.wlz
12034_wholemount_possible.wlz
12034_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12034_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4476
Entity Detected:Csrp2bp, cysteine and glycine-rich protein 2 binding protein ( MGI:1917264)
Sequence:sense strand is shown

>T4476
GGAGCGAGTCCGCGGCCGGGAGCGGGAATGTCGAGGAGTTCGAAGGCAGTGCTGGGCCTCTCGGTGCTGC
TGACGGCGGCCACGGTGGCCGGCGTGCATCTGAAGCAGCGGCAGGACCGGCAGAGGCTTCGTGACGGAGT
GATCAGAGACATTGAGAGGCAGAATCAGAAAAAAGAAAACATTCGTCTTCTGGGAGAACAGATTATTTTG
ACTAAGCAGCTTGAAGCAGAAAGAGAGAAGATGTTGGAGAAAGGATCTCAAAATACCTGACTTTCAGAAG
CCGGGGGCAGCGCTGAGTTGGTGTGCGTAACTGTGGGTGTGGTGTGTTGGTAGAGAGTTGTTAGTGAGAA
GAGCTGCCCCCCCAAATCACGCTCCATTTAAACTTTGTTAAATGAAGGACAGTGGGCCCTGTCTTCTAAA
TTGCTTCTTTAAAGAGTCTGTTCTGTCTGGATAGAGTGCTTGTGGGCAGATGTCCTTCTGAAGGTCTGGG
GGATCCCGGCAGCTTAGGAGTGCGTGTGAGCAGGGGGCGATGGATAGTAGCATCCACCTGAGTGGGCTCC
TCAGTCGGCATGACGATGACGCCACGAGAACGTCCACCTCGGAAGGGCTGGAGGAAGGCGAGGTGGAGGG
AGAGACTCTCCTAATTGTGGAATCGGAGGATCAGGCGTCAGTGGACTTATCTCACGACCAGAGTGGGGAT
TCCCTCAACAGCGATGAAGGAGATGTGTCCTGGATGGAGGAACAGCTGTCTTACTTCTGTGACAAGTGCC
AAAAGTGGATCCCAGCCAGTCAGCTGAGGGAGCAGCTCAGTTACCTGAAGGGGGATAACTTTTTCAGGTT
TACTTGTTGCGATTGCTCTGCAGACGGCAAGGAGCAGTACGAGAGGCTGAAGCTGACATGGCAGCAAGTG
GTCATGCTGGCAATGTACAACCTGTCTCTGGAAGGAAGTGGACGTCAAGTTTATTTCCGGTGGAAAGAAG
ATATCTGTGCTTTTATTGAGAAACATTGGACTTTTTTACTAGGAAATAGGAAAAAGACGTCGACGTGGTG
GAGCACAGTAGCAGGCTGTCTCAGCGTGGGAAGCCCTGTTTATTTCCGGTCAGGTGCTCAGGAGTTTGGA
GAGCCCGGATGGTGGAAACTTGTTCATAACAGACCCCCAACCATGAGGCCGGAGGGAGAGAAGCTGGCTG
CCTCCACCTTGAAAGTGAAAGCTTCAAAGCCAACGCTGGATCCCATCATTACTGTTGAGGGACTCAGAAA
ACGGGCAAGCCGGAATCCTGTGGAATCTGCCATGGAATTGAAAGAGAAGCGGTCGCGAACACAGGAAGCT
AAAGACATCAGAAGAGCTCAGAAAGAAGCGGCTGGCCTCCTCGACAGGAGCACCTCTTCCACTCCCGTCA
AGTTCATAAGCCGAGGCCGGAGGCCGGATCTGATTTTGGAGAAAGGAGAAGTGATTGACTTCTCGTCCCT
AAGCTCCTCAGACCGCACTCCCCTCACAAGCCCGTCTCCTTCTCCGTCTCTGGATTTCTCCGCCCCGGGG
ACGCCAGCCTCGCACTCGGCCACACCTAGCTTGCTTTCCGAAGCAGACCTCATCCCAGACGTGATGCCAC
CCCAAGCCCTGTTTCATGATGACGATGAGCTGGAAGGAGATGGAGTTATAGACCCAGGGATGGAGTACAT
CCCACCCCCAGCTGGGTCAGCGTCTGGGCTGCTTGGGAGCAGAAAGAAGGTCCGAGCTCCGGAACAGATA
AAACAGGAAGTGGACAGCGAGGAGGAGAAGCCAGACAGGATGGATGGAGATAGCGAAGACACAGATTCAA
ACATTTCTCTGCACACTAGAGCTCGAGAAAAGAGGAAGCCGCCACTGGAAAAGGACATGAAGCCCAAGGG
CCCCAGGTATACACCCGTGAGCATCTATGAGGAGAAGCTGCTGCTCAAGAGGCTGGAGGCGTGCCCCGGC
GCCGTGGCCATGACTCCAGAAGCCAGGAGGCTGAAACGGAAACTGATCGTCCGGCAATTGAAAAGAGATC
GGGGTTTACCACTGTTTGACTTGGACGAGGTTGTGAATGCCGCACTTCTGTTAGTCGATGGGATTTATGG
AGCCAAAGATGGAGGAGCTTCCCGGCTTGCAGCTGGACAAGCTACATACCGGACCACCTGCCAGGACTTT
CGGATCCTGGACCGGTACCAGACTGCCTTACCAGCCAGGAAAGGATTCCGGCACCAGACCACGAGATTTT
TGTATCGTTTGGTGGGATCAGAAGATCTGGCTGTGGACCAAAGTATTATCAGCCCTTATACTTCTCGGAT
CTTGAAACCTTATATCAGGCGTGATTATGAGACAAAGCCACCCAAACTACAGCTCCTGTCACAGATCCGT
TCCCACCTGCACAGGAGTGACCCTCACTGGACACCTGGACCTGATGCACCCCTCGATTACTGCTATGTCC
GACCAAATCACATCCCAACCATCAACTCCATGTGCCAGGAGTTTTTCTGGCCTGGCATTGACCTGTCTGA
GTGTCTGCAGTATCCAGACTTCAGTGTTGTAGTCCTTTATAAAAAAGTCATTGTTGCCTTTGGCTTCATG
GTTCCTGATGTGAAGTACAACGAAGCTTACATCTCATTTCTGCTTGTTCATCCTGAGTGGAGGAGAGCAG
GGATCGCCACATTCATGATCTATCATCTGATTCAGACATGCATGGGCAAGGATGTGACTCTTCATGTCTC
GGCAAGCAACCCCGCCATGCTGCTGTACCAGAAGTTTGGCTTCAAGACCGAGGAGTATGTTTTGGATTTT
TATGATAAGTATTACCCGCTGGAGAGTACGGAGTGTAAGCATGCATTCTTCCTGAGGCTCCGACGCTGAG
GGGAGCTCTGCTCTTCCCAGCAAACGGTGGATGCTGCCAGAGAGTGGGCTTCAGAAGCCCTGCAGGCCCT
TATCCTCGAAGTGGGCCCTGGTCTCCTGTGGGTAATAGCCTCCACATGGTCCAGCCGGGTGAGTGAAGCC
AGTGGTACAAGTAGGCAGTTCTGCCACCCAGAAACCATCCAGCAGGCCCCGCGTGGCCTTCCAGCAGTAT
CGTGGACTCCACTGTAGACCAGAACCAAGTGACTCTGCCATTCTCTTCCCATACAATGAGTGAGCACAAA
ATAGCCTCTAAGCTGAAGAGTTTGAGAATGGGCCTGCTCGTCCTACGTTAGAGCTCAGGAAGTATGAAAG
GTGTTAAAGATTGCGGTGCCCTCGCTGTCCCAGGACGAGAGGTTGTGAGGGATCAGGAATGATTGCAACC
CTGTTCTCAGCCTGTGCTGTGTTGTCATTGAACCCCAGAGGACTCACGGGGCACTGAAGTCCTTTTGTCT
CTTTCTGACTTTTGTCCCGTTCAGGAAGAATATACTCAGTGTCTGTCTCCACGCTGCTTTACCTGATCTA
CCTGTCAGCAGCTCCTTGCAGGGACACTGACCTGATACAAGGAGTTTACTCTTGGCATGGCCTGGAAAAT
AGCTTGAGAGGAACACTTGAGCTTGCCAGGCCCGTCTCGTGCTCAGGACAGTTGCACACAGGAGTGTCGG
TGTGGAACGGTGCAGGCAGATCTGGCTGCGCTCGCTGTCCACCTCACGAAGCTGCTTCCTGTGTTGCACA
TGTGGAGTTGGACTACAGGTATATCTTAAGTGCATTCCAAAAGCATCAGATATTCTCCAATAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4949236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4949236 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12035 same embryo
 EMAGE:12033 same embryo
 EMAGE:12037 same embryo
 EMAGE:12036 same embryo
 EMAGE:12038 same embryo
 EurExpress:euxassay_008407 same experiment
 MGI:4824090 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS