EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12038

Snx14 sorting nexin 14 ( MGI:2155664)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008418. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008418_01	euxassay_008418_02	euxassay_008418_03	euxassay_008418_04

euxassay_008418_05	euxassay_008418_06	euxassay_008418_07	euxassay_008418_08	euxassay_008418_09

euxassay_008418_10	euxassay_008418_11	euxassay_008418_12	euxassay_008418_13	euxassay_008418_14

euxassay_008418_15	euxassay_008418_16	euxassay_008418_17	euxassay_008418_18	euxassay_008418_19

euxassay_008418_20	euxassay_008418_21	euxassay_008418_22	euxassay_008418_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12038_wholemount_strong.wlz

12038_wholemount_moderate.wlz

12038_wholemount_weak.wlz

12038_wholemount_possible.wlz

12038_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12038_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4475
Entity Detected:	Snx14, sorting nexin 14 ( MGI:2155664)
Sequence:	sense strand is shown >T4475 GGGCCTTGTGCGGCTGAGTCTAGCTGTGAAGCTGGGTGGTGGGTGGCAATGTGGCTCCGCCGCCGGCGTT TGGGGGTGCCCTCCGCCTCTGCCTCTGAGGGCGGCACGTCCTCCCCTGCGTGGACTGAGATGGGGTCTTG GGTGCGGACGATCTGCGGGAGGCTGAAGCAGCGGCTGCGGCTGGACGTGGGGCGCGAGATTTGCCGTCAG TATCCGCTGTTCTGCTTTCTGCTGCTCTGCCTCAGCGTCGCCTCGCTGCTCCTCAACAGGTATCTGCATG TCTTAATGATCTTCTGGTCGTTTGTTGCTGGAGTTGTGACATTCTACTGTTCATTAGGACCCGATTCCCT CTTACCAAATATATTCTTCACAATAAAATACAAACCCAAGCAATTAGGACTTCAAGAGTTATTTCCGCAA GGACATAGCTGTGCTGTTTGTGGTAAAGTGAAATGTAAACGGCATAGGCCTTCCTTGCTCCTTGAAAACT ATCAGCCCTGGCTAGACCTGAAAGTTTCTTCGAAGGTAGATGCATCTCTCTCAGAGGTTCTTGAATTAGT GTTGGAAAACTTTGTCTATCCATGGTACAGGGATGTGACGGATGATGAGTCCTTTGTTGATGAGCTGAGG ATCACCTTACGGTTTTTTGCATCTGTCTTGGTGCGAAGAATTCACAAGGTGGATATTCCATCTATTATAA CCAAGAAACTATTAAAAGCAGCAATGAAACATATAGAAGTGATTGTTAAAGCAAGACAGAAAGTAAAGAA CACGGAGTATTTACAACAAGCCGCTTTAGAAGAATATGGTCCAGAGCTTCATGTGGCCCTGAGAAGTCGA AGAGATGAATTACAGTATTTAAGGAAGCTTACTGAGTTACTTTTTCCTTATATTTTGCCTCCTAAAGCAA CAGACTGCAGGTCCCTGACATTACTCATAAGAGAGATTCTGTCTGGTTCTGTGCTTCTTCCTTCTTTGGA TTTCCTAGCTGATCCAGATACTGTCAATCATTTGCTTATCATCTTCATAGACGACAGCCCTCCTGAAAAA GCAACTGAACCAGCTTCCCCTTTGGTTCCATTCCTGCAGAAATTTGCAGAACCTAGAAATAAAAAGCCAT CCGTGCTGAAGTTAGAGCTGAAACAAATCAGAGAACAGCAGGACCTTTTGTTCCGCTTTATGAACTTCCT GAAACAAGAAGGGGCCGTGCACGTCCTGCAGTTCTGTCTGACAGTCGAGGAATTTAATGATAGAATTTTG CGTCCAGAGTTATCAAATGATGAAATGCTATCTCTCCATGAAGAATTGCAGAAGATTTATAAGACCTACT GCTTGGATGAAAGCATTGACAAAATTCGCTTTGATCCCTTCATTGTCGAAGAGATTCAAAGAATTGCTGA AGGCCCATACATAGATGTTGTGAAGCTGCAAACTATGAGGTGTCTTTTTGAAGCATATGAGCACGTCCTT TCCCTGTTGGAGAACGTGTTTACCCCCATGTTCTGCCACAGTGATGAGTATTTTAGGCAACTTTTAAGAG GTGCAGAATCGCCCACACGCAATTCCAAATTCAACAGAAGCACACAAAAAAGAGGAGAATCGTTTGGAAT CAGCAGAATAGGTAGCAAAATTAAAGGCGTATTCAAGAGTACGACAATGGAGGGAGCTGTGCTGCCTAAT TACGGGGTGGCTGAAGGCGAAGATGACTTTATTGAAGAAGGGATTGTGGTAATGGAGGATGACTCTCCAG TAGAAGCTGTGAGCACACCTAACACTCCTCGAAACCTTGCTGCCTGGAAAATTAGCATTCCGTATGTAGA CTTTTTTGAAGATCCCTCCTCTGAAAGGAAAGAGAAGAAGGAGAGAATTCCTGTGTTTTGTATCGATGTT GAAAGAAACGACAGACGAGCAGTTGGACACGAGCCTGAGCATTGGTCTGTCTATAGAAGATATCTTGAAT TCTATGTTCTTGAATCAAAACTAACAGAATTTCATGGCACATTTCCTGATGCTCAGCTTCCATCCAAAAG GATCATTGGCCCCAAAAACTATGAGTTTTTGAAGTCGAAGAGAGAGGAGTTCCAGGAGTATCTGCAGAAA CTTGTGCAGCACCCGGAGCTGAGTAACAGCCAGCTTCTGGCTGACTTTCTCTCCCCCAACGGTGGGGAGA CACAGTTTCTAGATAAGATACTTCCAGATGTAAATCTTGGAAAAATTATAAAGTCTGTTCCTGGGAAACT AATGAAAGAGAAAGGTCAGCATTTGGAGCCATTCATCATGAGTTTCATTAATTCTTGCGAATCTCCCAAA CCTAAACCGAGTCGGCCAGAACTGACGATTCTCAGCCCCACTTCCGAAAACAACAAGAAGCTTTTCAACG ATCTGTTTAAGAATAATGCAAACCGCGCTGAGAACACAGAGCGAAAGCAAAATCAGAACTACTTCATGGA GGTGATGACTGTGGACGGAGTCTATGATTACCTGATGTATGTAGGGCGAGTAGTTTTTCAAGTCCCAGAC TGGCTTCATCATCTTCTAATGGGAACTCGAATCCTCTTTAAGAACACCCTGGAAATGTACACAGACTACT ACCTCCAGTGCAAGCTGGAGCAGCTGTTTCAGGAGCACCGGCTGGTCTCCCTCATCACCCTTCTCAGAGA TGCTATATTTTGTGAAAATACTGAACCTCGCTCTCTCCAAGATAAGCAAAAAGGAGCAAAACAGACTTTT GAAGAAATGATGAATTACATTCCAGATCTGATAGTCAAGTGTATCGGTGAAGAAACCAAGTACGAAAGCA TCAGACTCCTGTTTGATGGCCTGCAGCAGCCAGTGCTCAACAAGCAGCTGACCTATGTGTTGCTGGACAT TGTGATACAGGAGCTGTTTCCAGAGCTAAATAAGGTACAAAAGGAAGCTACCTCCATGACATCCTGGATG TAAACACTTGGTATAGAATAACATACCAGAATAATAGAAATGTACTTTTCACTTTGTATATTTTTATAAA TATCATGTACTTTAGTGTTTGGAATATATTTTTTGTTTTAATAAAGACTAACAAATAGCAATAAAAAGTG GTTTAGGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4948538 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4948538 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12035 same embryo
	EMAGE:12033 same embryo
	EMAGE:12037 same embryo
	EMAGE:12036 same embryo
	EMAGE:12034 same embryo
	EurExpress:euxassay_008418 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source