EMAGE mouse gene expression spatial database

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EMAGE:12050

St6galnac4 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 ( MGI:1341894)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009555_01	euxassay_009555_02	euxassay_009555_03	euxassay_009555_04	euxassay_009555_05

euxassay_009555_06	euxassay_009555_07	euxassay_009555_08	euxassay_009555_09	euxassay_009555_10

euxassay_009555_11	euxassay_009555_12	euxassay_009555_13	euxassay_009555_14	euxassay_009555_15

euxassay_009555_16	euxassay_009555_17	euxassay_009555_18	euxassay_009555_19	euxassay_009555_20

euxassay_009555_21	euxassay_009555_22	euxassay_009555_23	euxassay_009555_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12050_wholemount_strong.wlz

12050_wholemount_moderate.wlz

12050_wholemount_weak.wlz

12050_wholemount_possible.wlz

12050_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12050_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9309
Entity Detected:	St6galnac4, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4 ( MGI:1341894)
Sequence:	sense strand is shown >T9309 GGGACACGTTGTCACATGCTTGGAGACTTAAAGGCTTTTGGTTCCTGGGCCCGTTTTTCTTGATCTGGAT TTGTCTCTTCCCTTGCCTCAGTTTCTCTCATATCCTGGGACCAGCTTCCAGCCTTTCTCATCCCAAGCAC TGGAGACAGCAGCCTCCAGACTGCTAAGAGCAGAGACAGCATGAAGGCCCCGGGCCGCCTTCTGCTCCTC ACACTGTGCATCTTGACATTCTCTGCTGTCTGTGTCTTCCTGTGCTGCTGGGCCTGCCTGCCGCTCTGCC TGGCAACCTGCCTGGACCGCCACCTCCCTGCGGCCCCCAGATCCACTGTGCCAGGGCCCCTGCACTTCAG TGGCTACAGCAGCGTGCCAGACGGGAAGCCTCTTATCCGAGAACTGTGCCACAGCTGTGCGGTGGTGTCC AGCTCTGGCCAGATGCTGGGTTCAGGCCTGGGTGCCCAGATCGATGGCGCAGAGTGCGTGCTACGCATGA ACCAGGCACCCACCGTGGGCTTTGAGGAGGACGTGGGCCAGCGCAGCACTCTGCGCGTGATCTCTCACAC AAGTGTGCCATTGCTTCTGCGCAACTACTCACACTATTTCCAGCATGCCCGAGACACGCTCTACGTGGTG TGGGGCCAGGGCAGGCACATGGACAGGGTGCTGGGCGGCCGCACCTACCGAACGTTGCTGCAGCTCACCA GAATGTATCCAGGCCTGCAAGTGTACACCTTCACTGAACGCATGATGGCCTACTGTGACCAGATCTTCCA AGATGAGACAGGCAAGAACCGGAGACAATCAGGCTCCTTTCTCAGCACCGGTTGGTTCACCATGATTCTG GCCCTGGAGCTGTGCGAGGAGATCGTGGTCTACGGGATGGTCAGCGATAGTTACTGCAGCGAGAAGAGTC CCCGCTCCGTGCCCTACCACTACTTTGAGAAGGGCCGGCTAGATGAGTGTCAGATGTACCGTTTGCATGA GCAGGCTCCAAGGAGCGCCCATCGCTTCATTACCGAGAAGGCTGTGTTCTCCCGCTGGGCCAAGAAGCGA CCCATCGTGTTCGCCCACCCGTCCTGGAGGGCCAAGTAGTTCCTGTTGCCAGTGACTGCCAGGCCTCAGC CAGGCCTGTGACCCATTCCAGGCCAACACGGCTCTAATGTGAGCATTTATGACTCACCTTCTACCTGACA CCAGGTAGAACATGAAGTCTCTATTACCCAACACTGGGTACACTCGGAGGAACCTCAGGCCTTGTGACTT GAAGAGGAGTCGAGAAGATGCCATTTACACCACCCCTGCTTCCAGAAGAATCCACATCTTCATCTTGGGG TTTGTCCCACTGCCAGGTTGTTTAAGTGACCTTTGCCCTGGGTCCGTCGATGGCCCCACCTCACCAGCAT TATGGCTTTATACCAGTCTTGAACCTTTCCTCTACCTGGCTCCAGCCACATGGTGCCACATTCTCAGGCT GGTGGCCCTGGTTGGTGCACCCTGGAACTTGGGATTTGATGGATGTGAGGGCCTTTGAGCCTCAGCCTCT CAGGACTGACTGTCTTGGGAGTGTGCTGGTAAAGGCAGATTTTCTGGACACTGTGTCTCTGTGGTAACGG ATGTGGGATGGGGGTTAGTTAGGACAAGGCCTGTAGCCTGTTCCCTGGAGGGCTGACTGAGCCTTCCAGC CCTTCTCTAGGCCCACAACTGTCCTGCTCTTCCCGCATTGTCTGGACATCAGCCCTACAGTCTCCTGCCC CAGCATCCCACAAGCCTGACCTTCTGATGGTCTGACCACAGGACTGTGCTGGCCCAGGACAGCCACCCTG CCCTCACTCAACAGGCACAGAGCGGGGCCTGGGAGGACTCGGGGTGAGGAAGAAATTCCACCCAACGCCA CTTTCCTGCCTCATGAGTCAGATTCCAAAGTCTTTGCCAGTCAGGCTGTCTCTTAACTGTGGGACATGAT TCCTTTGTAAAACAGAGGGCAGAACGAACTCAGCGCGGGGAGGGTGCTGACCAGAAAGGAGTGGTCTCGG GGCCAGCCCTTTCTCTGAGTTTTGCTTTTCAGTACTAAAACACAGCCACCACCTAGCTGTTAAGGAGGGT GTGAGGCCACCCTGCGGAGGGAGCTGGTGCTCATTACAGATGATATAGCCCAGGCTAGCTTTCAGGAGGT GTCTGAACTAGGATGCTGGCAGCCTTGAGAGCTCACTACCCAGGCTCTGCCTCCTTCCCCCTTTGGAAGA CTGAAGAATGGCACCCTGGGCTTTAGCTCTCTGCTTCAGAGTTCGGGACCCTCTGGGTGTAACCTGCGCG GTCCCCAGAGTCCCGTGCTTAGTTTAACCTCTCCTGCTACAAACTTGAAATTCCTTATTTTAGGTCTTGG TTTGAAACAAGGAACCTCTCAGTTTCCTTACCGGATCCCACAAAGGGCAGAACCCGTCATGCTAAGGGCT CTGGTGGCCTAGCCCACTCTCCCTCCTACCCATTTTCCCAGGAGTGACTAGAGACCTGGCACCTGGCCCA CAACACTGCCCTTGTCCCTGTCCAGTGCCAGGTGGACCGGAAGCCTCATATGTGGGAGGGGAAGCAGAGA GGGAAATCTCTGAGCCAGGGTTACAGGCGGGAAGCCAAACCGTTTCCGCAGGCCTGGAGGGGCTGAGGGG CTGAGTGTAGGGATAGGCCATGAGTGGGTCGTGGGTGAGACGAAGGGTCCTGGCATCAGGCCTGACCCCA TGTTCCCAGGCAATAAAGACAGTAGAGTAGTTCCTGCCTCACCAGATTGTATGAGCTCAGAGATGCTGGG GTGCCCCTTGGGGCTTACTTAGAACACACTTGGCTCTCAGTATTCAGAAGCCACCATTTAAATAGGGTGG CCCTCTGCCTGACTGATCCTGTTCCACCCTGGCATAGGTGGCATCAGTGCAAAATAATAATAATAATAAT GATGATGTTCTCATCCTAATGAGAATAGTGTCTTCTGGTGCCATTTTGAGTGACTGTGACTCAGGAACAG AGATGTATGTTACCCCGAATTCCATGTTCCGTTGCGGAAACAGTTTCATGAAGTTTTATAGTTTTGCGGA ACATAAAGTCCTAAGTCCAGGCAAGTCTAAAATACATTGGTGGGGACATCAGAAAGGTGGGTACGGCAAG CTGGGGAGGACTTTCCTATAAGGTCTGAGAGGTTGAGAGCTTTGGGTTGGTAGAAGCTATGTTCTGACAA AGGTAGTCAGGAAATGGCTGTGTGAAGCTACAGCTAGCCCAAGATCTGCTGATTTTGGCCTCTGGGCCAT CTCCGGAGGCCTGGTTTCTTTCTAGGACTTTTTGCTCATGGTAGAGAGCTGGCTCTCCATCCCATTAGAA GTCTCCGCACCTGTGCCCTTCTCCGTGCATGTCCAGGGTCAAGAGACTCCCTGGTCCTGGTGGGATCTAG ACTACTCCTTTCTGAGAGTCAGTCTTCCTGCTTTCAGCCATAAGGAAATGCTGACCCAACCCTTAGACAA GGCACACAGAGATGCTTCTGTCCTGGCTTCTGGGTGAGGTGTTTACTGCTGGGTTGGCCTGAATAAATTG TATTTCTCAGGGAAGCTGTCAACCACTTCCTCCAAGCACACAAAGATGCCCACTGACAGGTCTGGCTGTT GGCACAGCGTCTACTGAAGACTCCCATGATGAAACTCAGGGGCCCCCTCCTTGGACACTGTGGGTCCGGT ACCTGCTGGAAGGTGAGAAGGGGGTAGAAGCCATGTATAGTCTTGAGGTGTTGTGGGGGCCACTGGGGTT TACAGACTCTGGCCTCTTGTGTTCCTTTTCCTCCTCCGGTTCTTTTGTCTCAGCCAGTTCTGCCTAAGCC CGGGGAGGAAGGGAGGCAGGTCCTACAGACACGGGTGGCTTAGGACTCCTTCATCAGGAATCTCGGCTGG GGTATGAGCGTCTCTATGGTGTCTATTGCGGGAGGAATGGAACTCCACGATTACGGGGAACAGTTGAGCA CTCCTGTCTAGCCCCTGTCTGTGGAGACCCAGGTACTTCCCAGTTCATGTCCACGGCACCAGGCTCAGAG CGCGGCTTAAGTCTCCATCTCCGCTCACTGTAGAGGTTGAAAGTTGGAGTCCCCAGGCCTGGCTTCAGCT GAGCTTGGCACAAATCTGTCTGCTGAACAGAACTGTTGCTATAAGTTGGGAGCTGAGGGGCTGGAGAGAT GGCTTAGCGGTTAAGAACACTGACTGTTCTTCCAGAGGACTGGGCTTTGAATCCCAGCAGCCTGATGGCA GCTCAAAACTGTAACTCCAAGATCTGACATTCTCACATGGACATACATGCAGGCAAAACACCAATACATA CAAAATAAAATTTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6844232 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6844232 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12054 same embryo
	EMAGE:12053 same embryo
	EMAGE:12051 same embryo
	EMAGE:12052 same embryo
	EurExpress:euxassay_009555 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source