EMAGE mouse gene expression spatial database

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EMAGE:12051

Fzd7 frizzled homolog 7 (Drosophila) ( MGI:108570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010965_01	euxassay_010965_02	euxassay_010965_03	euxassay_010965_04	euxassay_010965_05

euxassay_010965_06	euxassay_010965_07	euxassay_010965_08	euxassay_010965_09	euxassay_010965_10

euxassay_010965_11	euxassay_010965_12	euxassay_010965_13	euxassay_010965_14	euxassay_010965_15

euxassay_010965_16	euxassay_010965_17	euxassay_010965_18	euxassay_010965_19	euxassay_010965_20

euxassay_010965_21	euxassay_010965_22	euxassay_010965_23	euxassay_010965_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12051_wholemount_strong.wlz

12051_wholemount_moderate.wlz

12051_wholemount_weak.wlz

12051_wholemount_possible.wlz

12051_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12051_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9312
Entity Detected:	Fzd7, frizzled homolog 7 (Drosophila) ( MGI:108570)
Sequence:	sense strand is shown >T9312 GGCGGGCGGTGCTGGACGCGGAGAGTCGCGGGCCGGGAGGACTCTCATGCGCCGGGCGCGGCGGCGCCTC CCTGTATCCAAGCCTCTCCCCAGCGCCTCGTCTTTTTCCTCCAGCTGAGAACGCCGCTGCACTCGCGACC GGCGATGCGGGGCCCCGGCACGGCGGCGTCGCACTCGCCCCTGGGCCTCTGCGCCCTGGTGCTTGCTCTT CTGTGCGCGCTGCCCACGGACACCCGGGCTCAGCCATATCACGGCGAGAAAGGCATCTCGGTACCGGACC ACGGCTTCTGCCAGCCCATCTCCATCCCGTTGTGCACGGATATCGCCTACAACCAGACCATCCTGCCCAA CCTGCTGGGCCACACGAACCAAGAGGACGCGGGCCTCGAGGTGCACCAGTTCTACCCTCTGGTAAAGGTG CAGTGTTCTCCTGAGCTACGCTTCTTCTTATGCTCTATGTACGCACCCGTGTGCACCGTGCTCGACCAAG CCATTCCTCCGTGCCGTTCCTTGTGCGAGCGCGCCCGACAGGGCTGCGAGGCGCTCATGAACAAGTTCGG CTTCCAGTGGCCAGAGCGGTTGCGCTGCGAGAACTTCCCAGTGCACGGTGCCGGCGAGATCTGCGTGGGG CAGAACACGTCCGACGGCTCCGGGGGCGCGGGCGGCAGTCCCACCGCCTACCCTACTGCTCCCTACCTGC CAGACCCACCTTTCACTGCGATGTCCCCCTCAGATGGCAGAGGCCGCTTGTCTTTCCCCTTCTCGTGTCC GCGCCAGCTCAAGGTGCCCCCCTACCTGGGCTACCGCTTCCTAGGTGAGCGTGACTGCGGTGCCCCGTGT GAGCCGGGCCGTGCTAACGGCCTCATGTACTTTAAAGAAGAGGAGAGACGGTTCGCCCGCCTCTGGGTGG GTGTGTGGTCAGTGCTGTGCTGCGCCTCGACGCTCTTCACGGTGCTCACCTACCTAGTGGACATGCGTCG CTTCAGCTATCCAGAGCGACCCATCATCTTCCTGTCGGGTTGCTACTTCATGGTGGCAGTGGCGCACGTG GCAGGCTTCCTGCTAGAGGACCGTGCCGTGTGCGTGGAGCGCTTCTCGGACGATGGCTACCGCACGGTGG CGCAGGGCACCAAGAAGGAGGGCTGCACCATCCTCTTCATGGTGCTTTACTTCTTCGGTATGGCCAGCTC CATCTGGTGGGTCATTCTGTCCCTCACTTGGTTCCTGGCAGCTGGCATGAAGTGGGGCCACGAGGCCATC GAGGCCAACTCGCAGTACTTTCATCTGGCCGCGTGGGCTGTGCCAGCGGTCAAGACAATCACCATTTTGG CCATGGGCCAGGTGGATGGTGACCTACTCAGTGGAGTGTGCTACGTGGGCCTGTCTAGTGTGGATGCATT GCGGGGCTTCGTGCTGGCGCCCTTGTTCGTCTACCTCTTCATCGGGACGTCCTTCCTGTTGGCCGGCTTT GTGTCTCTCTTTCGCATCCGCACCATCATGAAGCACGACGGCACCAAGACAGAGAAGCTGGAGAAGCTGA TGGTGCGCATCGGCGTCTTCAGCGTGCTCTACACGGTGCCGGCCACCATCGTGTTGGCCTGCTACTTTTA TGAGCAGGCCTTCCGAGAGCACTGGGAACGCACCTGGCTCCTGCAGACTTGCAAGAGCTACGCTGTGCCC TGCCCTCCGGGCCACTTCTCTCCCATGAGCCCCGACTTTACAGTCTTCATGATCAAGTACCTGATGACCA TGATCGTGGGCATCACTACGGGCTTCTGGATCTGGTCGGGCAAGACCCTGCAGTCATGGCGTCGCTTCTA CCACAGACTCAGCCACAGCAGCAAGGGGGAAACTGCGGTATGAGCCCCGGTCCTTACCCACCCTTGCCTC TTCTACCCTTTTACAGGAGGAGAGGCATGGTAGGGAGAGAACTGCTGGGTGGGGGCTTGTTTCCGTAAGC TACCTGCCCCCTCCACTGAGCTTTAACCTGGAAGTGAGAAGTTATTTGGAGGTGAGAAGAGATTTGGGGC GAGAGATGGTTTTGAGAGGAGGCCCAGATGAAAAAAGGCAAAGGCAGTGGCCGAAAAGACTTCTGGCTAA GACTTGCAGGACGATGCTAACTGTGAAAGATATGGACCGGCTAGGGCCTAAGGGAAAGGTTGAGACCAGC AGAGAGAGAGACTGGTGAGGTTTTCAGGCGCCAGAGATGAGCCAGGGCTGTGAGTCCAATCCCCTGCTGC AAGGCAAGTGGTTGTTCTCACTCTAGTGAAGGGGGGCTGGGAGGGGAGGGTGATACCGCTCTGTCTGTAG CCTAGGCTTTGTGGCCAAGATGGGGGGGACCTCCTGCGGTGCCCTTGTCAAGTGGTGGTCAAACCATAAT CTCTTTTCACTGGGGCCAAACTGGAGCCCAGATGGGTTAATTTCCAGGGTCAGACCTTACAGTCCTCCTC CCGGGCCCCCTCCCGCCTGCTTTTCCTTCCCTACTCCTTTCAAGTCTAGTAAAATAAGCATTTGGAAGGC CGGGCCCTGCCTGCTAGAGTCCTAGCGTGAAGTTGGTTTTCAAGAGGAGGCCAAGAAGGCGAGTGGGAGA TACAGTCTGCTACTTTTTAATTTGTTGCTACTTTTTCATTTTCTAGGGAAGGCAGAGAGAAAAAGAATGT TTTATTTGGTTTCATACCCTGAAAAAAAGTCATGACTTGTTGCTTTTCAAAACAGGAACGCATTCACACA CACACACACCCCATCCCACCCCCCTTGTCTTTGTTGTAAGAGACAAAGCGGGAAACAAAAGTGTCTCCCT GAGGAAAGGCCTAACTGTGAAGCCAGCAGCTTTTACAGGCAAAGCCACAGAAATCCGAGGTTTTCCTTTG GTTGTTAATTTGGTTGAGATAAACATTCCTTTTTAAGGAAGAGTGAAGAGCAGCTTTCATACCCATTCAG GCACACGTTCTGACTTGGATAAAGGAAATGCTAGGAGTTTTGTTATTTGTTTTAAACAGATTTAATTCAG AACACATGATCTAATAGACTCTTTTGCTTAATGAAATCTCCTCCCATTCTACGCCCCCATAACCCAAATT TTGATTTTTCTGCCCCCTTCCTTCCGTCCAATTTGGGATTTTTGCTGTTTTTGTTTTGTTGTGTTTTGTT TTTCCTCCAGACAGGGTATCTCTGTGTAGCCCTGACTATCCAGGAACTGGCTCTGTAGAGCAGGCTGGCT TTGAACTCACAGACATCCACCTGCCTCTGCCTCTCAATTGCTGGGATTAAAGGCATGGTCCACCAGGCCT GGCTCCCCTTCCTAATTTGTATCTTTCAAGACATAACGCTCACATTAGTAAAGATAAAAGACAAAAATTT TAACTTAAAGGTTTAAAAGCATTTTGCCCTCATTTTCTTGTTCTAGAGATGTAAACATCTATCTATCAGA CACATGAGCTGACCCTTTCTCTCTCTGGTTGCATGAGGCGAGGGCAAGAGGAATGATAGCGAAGGAAGAG GAGAGTTTGAGTCAGGTTTCAAGAAAGTCATTAAGGGAAGGTGAACTTCAAAGTGATTCTGGAGTTCTTT GAAATGTGCTAGAAGACTTACATTTATTAATCTTAAATCGTGTACTTCTCTTTTTTTCCCTGCAATAGAA GTCTGGTTCTTTGGCATAATGTAAAGCTGAGCAGAGCATCATGGGAGTTGACCTTTACCCTACCTTTGAC ACTGACTGTCCTCCAATTTTGCAATGTTCTTCAGTTTCTCAGAGCAGTTTTTAATGCCAGCCAGGGGGGG ATTGTCGGGAGGACAGATTACTTCATATGTGTCCTGTTCAGTTGAATGGAGCTGCTTTTACAATTAAAGT GATCTTGTATTTTTTAAACTTTCAAAGTGATCTCACCTGTCAGAATTTTTTTAAGCTGCCACTACACAGG TTTGGCATCTTTTGTGTTTTATCTCTTTAAGTGCATGTGAAATTTGTAAAATAGAGACAGTGCAGTATGT ATATTTTGTAAATCTCCCATTTTTGTAAGAAAATATATATTGTATTTATACATTTTTACTTTGGATTTTT GTTTTGTTTACCTTTAAAGATCTACAATGAAGCCCCACTTTATCACATGTACAGATCACGAATAAATTTT TTAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6844727 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6844727 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12054 same embryo
	EMAGE:12050 same embryo
	EMAGE:12053 same embryo
	EMAGE:12052 same embryo
	EurExpress:euxassay_010965 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source