EMAGE mouse gene expression spatial database

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EMAGE:12068

Pcdhga9 protocadherin gamma subfamily A, 9 ( MGI:1935226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008609. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008609_01	euxassay_008609_02	euxassay_008609_03	euxassay_008609_04

euxassay_008609_05	euxassay_008609_06	euxassay_008609_07	euxassay_008609_08	euxassay_008609_09

euxassay_008609_10	euxassay_008609_11	euxassay_008609_12	euxassay_008609_13	euxassay_008609_14

euxassay_008609_15	euxassay_008609_16	euxassay_008609_17	euxassay_008609_18	euxassay_008609_19

euxassay_008609_20	euxassay_008609_21	euxassay_008609_22	euxassay_008609_23	euxassay_008609_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12068_wholemount_strong.wlz

12068_wholemount_moderate.wlz

12068_wholemount_weak.wlz

12068_wholemount_possible.wlz

12068_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12068_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

limb

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

gland

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

alimentary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nervous system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

integumental system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

sensory organ system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

cardiovascular system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

liver

moderate

single cell

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urinary system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

reproductive system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

respiratory system

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

tail

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9410
Entity Detected:	Pcdhga9, protocadherin gamma subfamily A, 9 ( MGI:1935226)
Sequence:	sense strand is shown >T9410 CAAAGCCCTGCACATCCTACAAACTGGCTCCGGGACTACAGCAAAATTCAACCTTCTAACTTGGGATGCT CTGGCTTTCTCCTGAGGAATAAGTATCATCTGACAGTTTGAGGAAGAAAAGAAGAGCCTAAGGAGTTAGC GAGCAGAAAGAGCATTCTGAGAAAATTCCGTAGCAAAAGCAACAATGGCTGCTGCCCCCGCCAGACGCCC GCACTGCAGAGAACTGGTCGTCTTGTGTGCACTGCTAGGGATGCTGTGGGAGGCCAGGGCCGGGCAAATC CGCTATTCCGTGCCAGAAGAGACAGAAAAGGGCTATATAGTGGGAAACATCTCTAAGGACCTGGGACTGG AGCCCCGTGAGCTGGCAGAGCGCGGAGTCCGCATCATCTCCAAAGGTAAGACCCAGCTTTTCTCTCTGAG TCCGCGGAGCGGCAGCTTGGTCACCGCAGGCAGGATAGACCGGGAGGAGCTGTGCGCTCAGAGCGCGACT TGTCTCCTAAACTTTAAAGTCCTAGTTGAGGACAGAGTGAAACTTTATGGGGTGGAAGTAGAAGTGGTTG ATATCAATGACAATGCGCCAAAATTTGAGGCCGAAAATTTGTTTGTAAAAATCAGTGAAATCGCTGCCCC CGGAGCAAGGTATCCACTCCCAGAAGCGGTTGACCCAGATGTGGGCATAAACTCCCTCCAGAGTTACCAG CTCAGCCCCAATCGCCACTTCTCTCTCCACCTGCAAACTGGAGACGACGGAACTATAAACCCAGAGCTGG TGCTGGAGCGCACCCTGGACCGAGAGGAAGAACCCACTCACCACCTGGTCCTCACCGCCTACGATGGCGG CAACCCACGCCGCTCCAGCACAGCGCTCATCCAGGTCACAGTGTTGGATACTAACGATAATGCCCCTGTT TTCGACCAACCGGTTTACCGCGTGAAAGTCCTTGAGAATGTGGCCCCGGGCACCCTGCTGCTCACAGTGA GAGCTAGCGACCCGGATGAGGGTGCCAATGGGAAGGTGACATACAAATTCCGGAAAATTAATGAAAAGCA TTCGCTGTTGTTTCATCTTCATGAAAACACTGGGGAAATGACAGTAGCGAAAAATCTGGACTATGAAGAA TGTTCGTTGTATGAAATGGAAATACAGGCTGAAGATGGTGGGGGACTGAAAGGGCGGACCAAAGTGGTAG TTATGGTAGAAGATGTAAATGACAATCGGCCGGAAGTGACCATTACCTCTCTGTTTAGCCCAGTAAGGGA AGATGCGCCCCCAGGGACAGTAATCCTCCTTTTCAATGCTCATGACCAAGATTCTGGGAAGAATGGGCAA GTTGTCTGTTCCATCCAGGAGAACCCATCCTTTAAATTAGAAAACTCAGTAGACGATTACTATAGGTTGT TGACAGCCCAGATCCTTGATCGAGAAAAGGCTTCTGAGTATAACATCACAGTGACAGCAACAGACAGGGG AACCCCATCCATGTCCACAGAAGTTCACATCACCCTGTATGTGGCTGACATCAATGACAATCCTCCTGCC TTCTCTCAAACCTCCTACTCAGTCTATCTCCCCGAAAACAACCCCAGAGGCACCTCCATCTTCTCAGTTA GCGCCCATGACCCTGACGACGAGGAGAATGCTAAGGTTACTTACTCTTTGGTGGAAAACACCATCCAGGG GGCACCACTCTCCTCCTACGTCTCCATTAATTCTGACACTGGCGTGCTCTATGCTCTGCAATCCTTTGAC TATGAGCAGTTTCAAAACCTTCAAATGCAGGTGAAGGCGAGTGACAACGGGCACCCGCCACTCAGCAGCA ATGTGTCCCTGTCTGTGTTTCTATTGGATCAGAATGACAATGCTCCCAAGATCCTGTATCCCTCCCTCCC GACTGACGGTTCCACTGGTGTGGAACTGGCTCCCCGCTCAGCAGAGGCTGGGTATCTGGTGACCAAGGTG GTGGCAGTGGACAAAGACTCTGGACAGAATGCTTGGTTGTCTTACCGTCTGCTCAAAGCTAGTGAGCCAG GACTTTTCACAGTGGGGCTACGCTCAGGCGAAATACGCACAGCTCGGGCCCTGCTAGAGAGAGATGCACT CAAGCAAAGCCTCGTGGTAGCTGTGCAGGACCATGGCCAGCCACCTCTTTCAGCTACCATCACTCTCACA GTGGCTGTGGCAGACAGCATCCCTGACATCCTAGCTGACTTGGGCAGTATCAACACTCCTGCAGACCCCG ATTCTGACATTACTCTCTACCTGGTGGTAGCAGTTGCTGTGGTCTCCTGTGTCTTCCTACTCTTTGTGCT AGTGCTGCTGGCCCTTAGGCTACGGCGCTGGCATGCTTCGCACCTGCTGCAGGATGCAATGGGCGGATCC ACTGGGGTACCAACATCACATATTGTGGGTGTGCATGGGGTTCAGACTTTCCTACAAACCTATTCTCAAG AGTTTTCCCTCACACCTGACTCTGGGAAGAGCCACCTGATCTTCCCCCAGCCCAACTACGCAGACACACT CATCAGCCAGGAGAGCTGTGGGAAGAGTGAACCTCTGTGCAGTTCAGTTGAGCCCAAGTTTCCTATTGAT GACACTCCTTTGGTTCCTCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGCA CGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCTGCA AGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGCACC ATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACGTGT ACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGCAGGTGG TAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAACCACAAG GCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGGCTCACC CCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCCAACACC CCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAATCAGTGA TTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACACACACAA ACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTATCCATCTCC TGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTGGGG TGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGGCCTCTCCT TCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCTGAGACCAA TGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTGGGAAGGCA TTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTGATGTTCCC ACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGTAGGGGGTG GTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCTGTTAATAG GGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATAGACAAACA CCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAATGGATTTA AGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGGGGCCTCTG TGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGGGGCCCAGC AAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAAAGTCCCAG CCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACCAGGTGCCA TTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTTAAAGCCCC CCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCCCCCTCCCC CGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCGGCGGGCGGT GGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGCTGT GTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCATAGTCACGT AGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGCTTTTTTCT TCGAAAATTAAAAATAAAAAAATGGTTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30536557 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30536557 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12067 same embryo
	EMAGE:12070 same embryo
	EMAGE:12071 same embryo
	EMAGE:12069 same embryo
	EMAGE:12072 same embryo
	EurExpress:euxassay_008609 same experiment
	MGI:4827091 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source