EMAGE mouse gene expression spatial database

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EMAGE:12069

Extl3 exostoses (multiple)-like 3 ( MGI:1860765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008608. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008608_01	euxassay_008608_02	euxassay_008608_03	euxassay_008608_04

euxassay_008608_05	euxassay_008608_06	euxassay_008608_07	euxassay_008608_08	euxassay_008608_09

euxassay_008608_10	euxassay_008608_11	euxassay_008608_12	euxassay_008608_13	euxassay_008608_14

euxassay_008608_15	euxassay_008608_16	euxassay_008608_17	euxassay_008608_18	euxassay_008608_19

euxassay_008608_20	euxassay_008608_21	euxassay_008608_22	euxassay_008608_23	euxassay_008608_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12069_wholemount_strong.wlz

12069_wholemount_moderate.wlz

12069_wholemount_weak.wlz

12069_wholemount_possible.wlz

12069_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12069_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

sublingual gland primordium

strong

regional

strong expression: see section 18

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 11 19 20 21 22

pancreas

strong

regional

strong expression: see section 11 12 13 14 15 18 19 20

thalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 15 16 17 18 19

olfactory cortex mantle layer

strong

regional

strong expression: see section 11 12 13 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 20

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 10 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 15 18 19 20 21

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 23 24

heart valve

strong

regional

strong expression: see section 14 15 16 17

renal cortex

strong

regional

strong expression: see section 10 11 12 13 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9237
Entity Detected:	Extl3, exostoses (multiple)-like 3 ( MGI:1860765)
Sequence:	sense strand is shown >T9237 CTTGTGCAGGGAGCACACTGATCCTTCCAGAAACTTGTGTGTGAAAAGAGGTTGCGTTTTGTCAGACAGA CTCATGGTTATGGCGAGCGATCCGACGTGATCAGAGTGGGCAAGAGGCACAGCGAACTCATGACAGGCTA TACCATGTTGCGGAATGGGGGAGTGGGGAACGGTGGTCAGACCTGTATGCTGCGCTGGTCCAATCGCATC CGGCTGACATGGCTGAGTTTCACGCTGTTCATCATCCTCGTCTTCTTCCCCCTCATTGCTCACTATTACC TCACCACTCTGGACGAGGCAGACGAGGCTGGCAAGCGCATCTTCGGCCCTCGGGCTGGCAGTGAGCTCTG TGAGGTAAAGCACGTCCTCGATCTCTGTCGGATTCGTGAGTCTGTGAGCGAAGAGCTTCTACAGCTCGAA GCCAAGCGGCAGGAGCTGAACAGCGAGATTGCCAAGCTGAACCTCAAGATTGAAGCCTGTAAGAAGAGCA TAGAGAATGCCAAGCAGGACCTGCTGCAGCTCAAGAATGTCATTAGCCAGACAGAGCACTCCTACAAGGA GCTGATGGCCCAGAACCAGCCCAAACTGTCCCTGCCCATCCGACTGCTCCCCGAGAAGGACGATGCCGGC CTTCCACCCCCCAAGGTCACTCGGGGTTGCCGCCTTCACAACTGCTTTGATTACTCTCGTTGTCCTCTGA CGTCTGGCTTTCCCGTCTACGTCTATGACAGTGACCAGTTTGCCTTTGGGAGCTACCTGGACCCTTTGGT CAAGCAGGCTTTTCAGGCTACAGTGAGAGCCAACGTTTATGTTACAGAAAATGCGGCCATCGCCTGCCTG TATGTGGTGTTAGTGGGAGAAATGCAAGAGCCCACTGTGCTGCGGCCTGCCGACCTTGAAAAGCAGCTGT TTTCTCTGCCACACTGGAGGACAGATGGGCACAACCACGTCATTATCAACCTGTCCCGGAAGTCAGACAC ACAGAATCTACTGTACAACGTCAGTACAGGCCGGGCCATGGTGGCCCAGTCCACCTTCTATGCTGCCCAG TACAGAGCTGGCTTTGACCTGGTCGTGTCACCCCTTGTCCATGCTATGTCTGAACCCAACTTCATGGAAA TCCCACCGCAGGTGCCAGTTAAGCGGAAATATCTCTTCACTTTCCAGGGCGAGAAGATCGAGTCTCTGAG ATCTAGCCTTCAGGAGGCCCGTTCCTTCGAGGAAGAGATGGAGGGCGACCCTCCGGCCGACTATGACGAT CGCATCATTGCCACCCTAAAGGCCGTACAGGACAGCAAGCTGGATCAGGTGCTGGTAGAATTCACTTGCA AAAACCAGCCGAAGCCCAGCCTGCCGACTGAGTGGGCACTGTGTGGGGAGCGGGAAGACCGCCTGGAGTT ACTGAAGCTCTCCACCTTCGCCCTCATCATCACTCCCGGGGACCCGCACCTGCTCATTTCATCTGGGTGT GCCACGCGGCTCTTCGAGGCCCTGGAGGTGGGGGCCGTGCCGGTGGTGCTCGGGGAGCAGGTGCAGCTCC CGTACCACGACATGCTGCAGTGGAACGAGGCCGCCCTGGTGGTGCCCAAGCCTCGCGTCACAGAGGTCCA CTTCCTGTTACGAAGTCTTTCAGACAGTGATCTGTTGGCCATGAGGCGGCAAGGCCGCTTTCTCTGGGAG ACCTACTTCTCCACCGCAGACAGTATTTTTAATACCGTGCTGGCCATGATTAGGACTCGAATTCAGATCC CAGCTGCTCCCATCCGGGAAGAGGTAGCGGCTGAGATCCCCCATCGTTCAGGCAAAGCAGCTGGAACTGA CCCCAACATGGCTGACAATGGGGACCTGGACCTGGGGCCGGTAGAGACAGAACCACCCTATGCCTCACCT AAATACCTCCGCAATTTCACTCTGACTGTCACAGACTGTTACCGTGGCTGGAACTCTGCCCCGGGACCCT TCCATCTTTTTCCCCACACACCCTTTGATCCTGTGTTGCCCTCTGAGGCCAAATTCCTGGGCTCAGGGAC TGGATTTCGGCCCATCGGTGGCGGGGCTGGGGGCTCTGGCAAGGAGTTCCAGGCAGCGCTCGGAGGCAAT GTCCAGCGGGAGCAGTTCACAGTTGTGATGCTGACCTACGAGCGGGAGGAAGTGCTCATGAACTCCCTGG AGAGACTCAACGGCCTCCCCTACCTGAACAAGGTAGTGGTGGTGTGGAACTCTCCCAAGCTGCCCTCGGA GGACCTTTTGTGGCCAGACATTGGTGTCCCCATCATGGTCGTCCGTACTGAGAAGAACAGTTTGAACAAT CGGTTCTTGCCCTGGAATGAGATTGAGACAGAGGCCATACTGTCCATCGACGATGATGCTCACCTCCGCC ATGATGAAATCATGTTTGGGTTTCGGGTGTGGAGAGAAGCACGTGATCGCATTGTGGGTTTCCCTGGCCG GTACCATGCGTGGGACATACCACACCAGTCCTGGCTCTACAATTCCAACTACTCCTGTGAGCTGTCCATG GTGCTGACGGGCGCTGCCTTCTTTCACAAGTATTATGCCTACCTGTATTCTTATGTGATGCCCCAGGCCA TCCGGGACATGGTGGACGAGTACATCAACTGTGAGGATATCGCCATGAACTTCCTTGTCTCCCACATCAC ACGGAAACCCCCCATCAAGGTGACATCAAGGTGGACTTTTCGATGCCCAGGGTGCCCTCAGGCCCTGTCC CATGATGACTCTCATTTTCACGAGCGGCACAAGTGTATCAACTTTTTTGTCAAGGTGTACGGCTATATGC CTCTCTTGTACACACAGTTCAGGGTGGACTCCGTGCTCTTCAAGACCCGCCTGCCCCATGACAAGACCAA GTGCTTCAAGTTCATCTAGGGCCTTGCCAGTTCTGAGGAGACGATGAGCAGAGCGAGGGGAGTCACCCTC AAGGTTCCCAAGGTGTCGAAGGTCCTTGGGGACATCTGTCGGGCAGGGCCAAGACCCTTTGCTGGGAGAG GCAGCAGGAAGAGTGGAAAGGGATAGCTGTCTTTCATTTTGAAGTCAGCCACACTGGGCCTGGGATCCTG GTCAGCAGACTCAGGGCGTCTGCACAGGGCACTGACTGATAGCGAACACTGAGGACTGTTCATAAGCCCA GGACAGCTGCTTCGTGGTTTTTAAATTCAGTAACAGCTATTATTATTTAAAGAGAGAATTTCACATCTGC CATTCAAGGCTTATTTATATGTGTGCGTATATGTACACACATATGTGTATATACGTATATATGCACACAC ACATACACACACATTCATACATACATTCATACATACATACATACATACACATATATATATTTTGGTGGGT CGGGGTGCACCTGAAATTTCTGCCAATCTCTCCAAGGGAGGGTCCTACCCAGCTATGTATTTAGTGGAGA GGGCCCTGGCCCTGCGTTACCTGGACATTCATTTGCGGTCCCCCCCGTCCCAGGGACACTGGCTGCAGAA ATAAGCAGGATGGGCCTGACCTAGGCAGGGTCTCCTGACCTCTCCTGGCCCCGTTCCAATGCCACAGTGC TGCTCCCACAGCTTGCCCCGCTGTAGGCTGCACAGTGGGGACGACACACCCCGGACGCTTTGAAGGGTTG GGCCAGGCCGCACTCAGGCATCACCTGGCTTTCCATTCTTTCTTGTGTTTGAGAGAGAAGGAGGCGGCAG ACAGTGAGGCAGAGATGAGACCTGTACGTAGCTCCCCCCCTCCCCCCCCCCCCGGGGTGACACTGGTGCA GGGTGCTGGTGGCCAGTTCCGTGGTACTTGTTTCTCACAGAGGAGACAACAGGAACTTGGCAGGCTGGCT GGGAGCTCGGCGGGTGTAGAGCCAGCCTGGGTCCTTCCGGGATTCTGTAGTCTTACTGCAAGGCTTTGGA AGGAACACCGAAGTGAAATTCACCTTCCCTCTTGTGTCTAGAAGTCCATCCTGTAGCTGTGGGCTTTAGA CCCCTGATGCCACGTTGGCTGTACGAGCCCCCCCTCCCCCCAACCTTCAAAGTCTAGGGTGGGCTAGAAC CCGGAGGCTGTGATTTGCTAGGTAAGTCCTAGTCTTGTCGTAGACGTTAACAGGAATTCACCCCACGTTA CCACTTTCCATGTCCTGTTGATGGTTTTCTTCCCAGGGCTGAAAATGAAATAAACTAACTTGGGGTATGG TGGCAGTGGGAAACTTAGGTCAGGGATTTGGCCTGTGACTTGTCTCCCCAGCCCCAGCAGCTGACGCAGA GCTCAGTCTGTCTTGGTCATGCACCTGCCTTAAGCCAGTTCCGTCTTCCTAGGCCCTGACACGTGTGCTG CTGCTTGCTGTCCTCTGCCTGCTGTCCTGACTTCTCTCTAGATCCCTCCTGCCGTAGGGCTGGCATGGCC CCCGGGAGACCCTCAGGCCAGCAGGACCAGGCTGCTGCAGAACTTCACTGGGTCTTCTGGAACCTGACAC CAAGGGCTGCCTGGGACATCTGGTTCTTGGCAACTCAGTGAAGGGGTCAAGACTGTTTCCCAACCTTCTG TCCTCTCATCAGCCCTTGTAGCAGTAGCCCTCTTCCCCCGGGGGAGCCAAAGAGTGTGGTGTTTTGCGCT AAATATGTGGCTGCTATTTTATCTTTTTTTTTTCCTCTCCCAGTATTGGGGATGGACGCCAGAGCTGTGG GTGGACATCCCGACAATCGCTCTGCCCTGAGCCCTGTCCCTATTGTCTCGTTTCTTTTCATTGAGAGGTT GTAAATGGTGCTGTGGTACCTGATAAGAAAGTCAGTCTCTGTGGTAGGACCCCCTCTGTGGGTCACTCAT GGGTATCATTGATCTGTGTTTGAGGGATAGTGATGTCAAGAGTGTCCTAGGGGATAGCCCTCTGGGCCTG GTGGGAACGCTCACTGAAGGACCCCAGCAGCAAACATTGGCAGTGACTGTACCTGAGGGACTCTTGTCAG AGGCAAGAGGCAGAGACAAAGCAGTTGGACCCTGCTTGTGATTTGGGTTTTGGGGTTAGGGACATGCTTG GAAATGATTGTGCCACTACACGTCCGTGGAGCCTGCCTGGTGTTTGACGTTGTTTACCATGGGTCACCTC CTAGAGCAGATTGCTAACACAGGTCACACTCAGCTACCTAGTCTCAGCTCCAGGGCCAGGACAGCTTTGT TTTCCAGTTGCTCTTCATCTTAAGGTCAAAGGCCACCCACAGAGTGACAGCCCCCCGGGATGCCTCAGGC CACCACCTAATGCTCCTGTCAGGGGTGGCCTTGTTAGTGAACATGGAGCTGGAGGGTGGGGCCGCCTGGG GTGGCTTTGATGACTTGTCCATCTGTCTGCCCCTTTCAAACCATGAGCACAAAGGTCTTACGTTGGATTT TTGTAAAAAACAAAAACAAAAAACAAAATAAATGGAGACTTTAACTCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834580 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834580 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12067 same embryo
	EMAGE:12070 same embryo
	EMAGE:12071 same embryo
	EMAGE:12068 same embryo
	EMAGE:12072 same embryo
	EurExpress:euxassay_008608 same experiment
	MGI:4824653 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source