EMAGE mouse gene expression spatial database

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EMAGE:12076

Ablim3 actin binding LIM protein family, member 3 ( MGI:2442582)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006983. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006983_01	euxassay_006983_02	euxassay_006983_03	euxassay_006983_04

euxassay_006983_05	euxassay_006983_06	euxassay_006983_07	euxassay_006983_08	euxassay_006983_09

euxassay_006983_10	euxassay_006983_11	euxassay_006983_12	euxassay_006983_13	euxassay_006983_14

euxassay_006983_15	euxassay_006983_16	euxassay_006983_17	euxassay_006983_18	euxassay_006983_19

euxassay_006983_20	euxassay_006983_21	euxassay_006983_22	euxassay_006983_23	euxassay_006983_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12076_wholemount_strong.wlz

12076_wholemount_moderate.wlz

12076_wholemount_weak.wlz

12076_wholemount_possible.wlz

12076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23 24

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 09 18 19 20

trigeminal v nerve

moderate

regional

moderate expression: see section 18

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

dorsal root ganglion

strong

regional

strong expression: see section 09 10 17 18 moderate expression: see section 08 11 16

neural retina

moderate

regional

moderate expression: see section 03 04 24

heart valve

moderate

regional

moderate expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9451
Entity Detected:	Ablim3, actin binding LIM protein family, member 3 ( MGI:2442582)
Sequence:	sense strand is shown >T9451 CGAGCTCTCAGGTTTCGCCTGGCTCGCCTTGGCATCCCAGACCCCAGACGACCCGCGGGATTCATGAGCC GCTTGCAGGGGCCGTGGCAGGGGCCTCTGGCTCCCAAAACTGGCCAAGAGGTGGGTCCCGGGGCGGGTCG CTGCTCCCGAGGCCGGGCGGGGACGCGCTGCCTCCGCCCTGCCTCCCTGTGGTCCACACCCCCTTCGGGC TCCGCAGCCTGCCGGCTGGGATAACGCGGGGTGCGCACGAGCCACCGCTGCCAGCCTCGCTACTGCCCGG GGTTCGGGTCCCAGGTGCTGAGGACCAGGCACCTGAGTCCTCATCGCCTGCCCCTCCTCCCTGTTCACCT GCCCTGTTCGTCCACTAGCCTTAAAGGCCAGCGGCTCCAGCCCAGACGGAGGGGCCAGCAGGGGGAGAGC GCACCTGAGGATACACAGCTGGCCCTGCTCTGCCTTTTCACCCCTAGGTGATGAGTGAGGCCAGGAGAAC ACAAGATTTAAAAAGCAACCGGGGCCTCCTCCGTATTGAATGAACGACCCAGAGCAAAGGCATCACCATG AACACGAGCATTCCTTACCAGCAGAGTCCGTACAGTCCCCGGGGCGGCTCCAATGTCATCCAGTGCTACC GCTGCGGAGACACCTGCAAAGGGGAGGTGGTCCGTGTCCACAACAACCACTTCCACATCCGATGCTTCAC TTGTCAAGTATGTGGATGTGGCCTGGCCCAGTCGGGCTTCTTCTTCAAGAACCAGGAGTACATCTGCACC CAGGACTACCAACAGCTTTATGGCACCCGCTGTGATAGCTGCCGGGACTTCATCACGGGTGAGGTCATCT CTGCCCTGGGCCGTACCTACCACCCTAAATGCTTCGTATGCAGCTTGTGCAGGAAGCCTTTCCCTATTGG AGATAAGGTGACCTTCAGTGGGAAAGAATGTGTATGTCAGACGTGCTCCCAGTCAATGACCAGCAGCAAG CCGATCAAGATCCGTGGACCAAGCCACTGTGCTGGGTGCAAAGAGGAGATTAAACATGGCCAGTCACTTC TGGCACTGGACAAGCAGTGGCACGTCAGCTGTTTCAAATGCCAGACCTGTAGCGTCATCCTCACTGGGGA ATACATTAGCAAAGACGGTGTTCCATACTGCGAGTCTGACTACCACTCCCAGTTTGGCATCAAATGTGAG ACTTGTGACCGGTACATCAGTGGCAGGGTCTTGGAGGCAGGAGGGAAACACTACCACCCTACCTGTGCCA GATGTGTACGCTGCCACCAGATGTTCACTGAGGGGGAGGAGATGTATCTCACAGGTTCTGAGGTTTGGCA CCCAATCTGCAAGCAGGCAGCCAGGGCAGAGAAGAAGCTCAAGCACAGGCGGACATCTGAAACTTCCATC TCACCCCCTGGTTCCAGCATCGGGTCGCCTAACCGAGTCATCTGTGCTAAAGTGGATAATGAGATCCTTA ATTACAAAGACCTGGCAGCTCTCCCCAAGGTTAAGTCCATCTACGAGGTGCAACGCCCCGACCTCATTTC CTACGAGCCTCATTCCAGATACACCTCCGACGAGATGCTGGAGAGATGCGGTTATGGAGAGTCACTGGGA ACATTATCTCCTTACTCCCAGGACATCTATGAGAACCTGGACCTCCGGCAGAGGCGGGCCTCCAGCCCTG GCTACATTGATTCCCCCACCTACAGTCGGCAAGGCATGTCTCCCACCTTCTCCCGCTCGCCTCATTACTA CCGCTCAGGGCCTGAGAGTGGCCGGAGCTCCCCATACCATAGCCAATTAGATGTGAGGTCCTCCACGCCA ACCTCTTACCAGGCTCCCAAGCACTTTCACATCCCAGCTGGAGAGAGTAACATCTACCGAAAACCCCCGA TCTACAAACGGCATGGTGATTTGTCCACAGCAACCAAGAGCAAAACAAGTGAAGACATCAGCCAGGCCTC CAAGTATAGCCCAGCCTACTCGCCAGACCCCTACTATGCTTCAGAGTCTGAGTATTGGACCTACCATGGG TCCCCCAAAGTGCCCCGAGCCAGAAGGTTCTCATCTGGAGGAGAGGAAGAGGATTTTGACCGAAGCATGC ACAAGCTCCAGAGTGGGATTGGCCGGCTGATCCTGAAGGAAGAGATGAAGGCGCGGTCAAGCTCCTATGC GGATCCTTGGACCCCTCCACGGAGCTCCACCAGCAGTCGGGAGGCCCTGCACACCACTGGCTATGAGATG TCCTTCAATGGCTCCCCTCGTTCACACTACCTGGCTGACAGTGATCCCCTCATCTCCAAATCTGCCTCAC TGCCTGCCTACAGGAGAAATGGGCTGCACAGGACACCCAGTGCGGACCTCTTCCACTACGACAGCATGAA CGCAGTCAACTGGGGCATGCGAGAGTACAAGATCTATCCTTATGAGCTGCTACTGGTGACCACAAGAGGA AGGAACCGACTGCCAAAGGATGTGGACCGGACTCGCTTAGAGCGCCACCTGTCCCAGGAGGAGTTCTACC AAGTCTTCGGCATGACCATCTCCGAGTTCGAGAGGCTAGCCCTCTGGAAGAGGAACGAACTGAAGAAGCA AGCCCGGCTTTTCTAGGCTGAGGCTCTATACATATAGATGCATTTATATAAAGATGTATGTAAATCTCTA TCCTGACACGCAGTATAATCCTCTTGTATAATGGGACACAACATGCTGCCTGCCGTGAGACTTGCTTTTC TGTACTGTCAGGCGAGCCCACATCATCGAGGTATTTTTATGCTCCTTACTTCTCTTTTCTATGTGCTGTG GGGTCTGGGAAGGAATCTGAAGGGACTCCATCCTTTTACTGGGGACCCTTTTTATACTGAAACATCTGTC CCACCTTGAGTCCCCAAGAACTGGCTCTCCTTTGAAAAGGTGCCTGGAGGAGCCTCTACTCCCTGCTTCA GGACCTTCCCCCAGTCTCCAGGGCTGACGCCGGCCACGAAGTGTTCACACACCGTGGTCACGGATGGGCC CTCCGGTGGGAGGTCCGTGGCAATCCACCCAAATCAGCGAGCACCTTTGAGGCCTCGTAAAGAATTCTCT TCACACATCCAAATAGGAGGAGCTCAGCGCTCCATGAGGTGATGTGGTTAGATGTGAGGTCCTCAAACCC ATTGCTTACATCAGTACAGAGTGGATGGGCTCTGGCCTCTCCTCGGCTCAGCACCCTTCCTCCTTCCACC CTTGGTCCTAAGCACTCCCTCATGCAAATCTAGAATTCATGGTAGGAAACGGAAAAAGGCACTTCCCTTC CCTCTACCACCCGCAACCGGCTCCTCTCCCCCACCTCAGTGGTCCCAGCACCCCCCAACCCTTTCCCCTT TCTAGGAGAGGTCATCGTTGCAGCCTGTACTCTTCAGCCTTATTACAATCTATGTGCCTGATGATCTACA CACCACAGGGCTAATGTTCCCACAATAAGCTCCAGCAAGCAACCAGACACACTCACCACCCTGTGCAGGG AGAATAGGCCACACCCCAAAGAAAAATCTTGGCCTAGGCCACTGGTCCCTAGAGGGCAAGACATTCATAT CAGACCCCCACCCATCATCCCCTGGCAATGCTATGACATTAGCGGGTCTCACAGTTGAAGTTTTTCCGAA GGGTGACTGTTGAGAGGATTCCAGAATGAGGCCTTGACCAACACTGCTCATGTTCTTTCCCGGTGGCCTC AGGGACTGACTCTCGTGCTTTCTGGGACAAGGGAGAACAGAGCCTTCTCACACCCCCACTTCAATTCTGA GTCATTGATCAGTGGTAGCTGTTCCCTCTTGGAGATGCCTGTGTCACAGTTCTTGGGGGGGTTGTGGATC CCAGGGGAAAGATGGAGAAGTTTAAAAGGACAATTACAACACTCAAGAAGCTGGTGATGTGACGTGGCGA GTGTAGAAATGACGTCAGCAAAGCAAACCGTTCCTGAGCACCTGCTGAATGCTGAGCACTGAGTGGGGGA GGGGGAGGGGCACCGGGATGATTCATAGAAGGCCCTAGTTTCCGGGGCATGCCTAGTAACGGGAGACCTG GTAAGACGTGTGCCAGACGTAACGTAACGTAACGTAACGTAACGTAACGGATGCAAGGCAGAACGTGACG GACGGAGGCCTGAGAAAGTGTGTGTGACGTTGCTGAAGGCGTGTGCAGCTGGTGCAGACCCAAGGAGGCT TCTGGAAGAGGTTGCATGCGAGCAGAAATCAAAAAGGATCAGAAGAATCTGCACATTGGAAGGGTGACGG GGCGGAGGGACTGCAAGGGACGTTTAATGTACTCTCATTTATGATGTCACCTCCCTGCAACTTCTCTTTT TGGCCAACGCCTTTCAACTTTCCCAATCCATTAGAAATGTCCCCCCGGCCAGATGCGATCATTGAAACTG CCTCATTGTCACTGGTTAACAACTTGCGAGATTGAAGGGCTTTTGTTATTGTTGTTGGTTAACAACTTGC GAGATTGAAGGGCTTTTGTTATTGTTGTTGGATATTTTTATTTCCCATAAAAGCACATCATTTCAACCCA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30534236 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30534236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12075 same embryo
	EMAGE:12079 same embryo
	EMAGE:12074 same embryo
	EMAGE:12077 same embryo
	EMAGE:12078 same embryo
	EurExpress:euxassay_006983 same experiment
	MGI:4822885 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source