EMAGE mouse gene expression spatial database

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EMAGE:12077

6030446N20Rik RIKEN cDNA 6030446N20 gene ( MGI:2442435)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007028. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007028_01	euxassay_007028_02	euxassay_007028_03	euxassay_007028_04

euxassay_007028_05	euxassay_007028_06	euxassay_007028_07	euxassay_007028_08	euxassay_007028_09

euxassay_007028_10	euxassay_007028_11	euxassay_007028_12	euxassay_007028_13	euxassay_007028_14

euxassay_007028_15	euxassay_007028_16	euxassay_007028_17	euxassay_007028_18	euxassay_007028_19

euxassay_007028_20	euxassay_007028_21	euxassay_007028_22	euxassay_007028_23	euxassay_007028_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12077_wholemount_strong.wlz

12077_wholemount_moderate.wlz

12077_wholemount_weak.wlz

12077_wholemount_possible.wlz

12077_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12077_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9465
Entity Detected:	6030446N20Rik, RIKEN cDNA 6030446N20 gene ( MGI:2442435)
Sequence:	sense strand is shown >T9465 GATGAACGTGAGGCGGTCCCTGCTCGGCCTCACCTTCTGCACCTGTTACCTGGCTTCTCACCTCACCAAC AAGTATGTCCTGTCTGTCCTGAAATTTACCTACCCTACATTATTCCAAGGGTGGCAGACATTCATTGGTG GACTTTTGCTTCATATGTCATGGAAACTAGGATGGGTGGAGCTCCACAGCAGTCCAAGATCTGATGTTTT GATATGGCTTCCTGCTTCAGCGCTGTTTGTGGGCATAATCTATGCGGGGTCCAAAGCTTTGTCTAGACTG GCTGTTCCTGTGTTTTTCATTTTGCATAATGTAGCTGAAGTTCTCACCTGTGGGTACCAGAAGTGTGTCT GGAAAGAGAAAACATCGCTCTCAAAGATCTGTAGTGCCCTCTTCCTCTTGGCTGCTGCAGGGTGCCTTCC CTTCCAAGACTCTCAGTTTGATCCAGATGGATATTTTTGGGCTCTTATCCACATCTTTTGTGTAGGTTCT TACAAAATACTTCGGAAGTCCCGGAAGCCCACTGTGCTAAGTGACATTGACCAGCAGTACTTAAACTATA TATTCAGCATGGTACTCCTGGCATTTGCATCTCATCCGACAGGTGACCTCTTCGGTGCCCTGGACTTTCC ATTCTTGTATTTCTACAGGTTCCATGGCAGCTGCTGTGCCAGTGGAGTTTTGGGATTCTTCCTCATGCTC AGTACAGTCAGGCTGAGAAGCATCCTGGCCCCAGGGCAGTGCGCAGCCTGGATCCTCTGTGCTAAGGTCG TCACAGCTGGCTTGTCGATGTTGCTGTTTGATATGGCCCTGACCAAAGCAACTGTGGGATGGTGCCAGCC TGCAGGGTGAGGAAAGTTGACCATATGCTACTGTTTGCAGCTTCCTGCTTGGTGGACTTGGCGAGGCCCT GCTGGTTTTCTCAGAGCGGAGGAGTTCCTCATGAAGATGGACAAGAAAAGGCAGCCTGCAGCTTGCTGCT GGAGGACACAGCCCCTGGCCTCCCTCTTGAGAGCTATAAAAAGACCACATTACAGTGAGACCATGCTGAG GTTTCCAGCTGGGCAAAGCTGTGAGCTGCACATGCCCCAGCCAAGTTGAACACATGGATGGCTGATGATT GCAACAGCCCACTTTCTTATTATTAGGGGGACTTCCCAGGGACCGCTGCAGAGAATGGGTCCGTCTTATA TGCTGAACCATCTCCTCTCTGCTGCACCCAGTTCTGCGGGATCAAGAAGCAGAGGGTCTTAATGGGATGG CTGTGTCCTACCAGTTACTGCTTTGGAATTGATGTCACAGTCCATGGAATCTTGTTGCATTTTGGGGAGT TGTTTTGTGTCTTTACATCTTGGAGTCTCTTATACCCTTTGATTTTTCTGGAGCCCATGCCTTCCCACAC AAAACAAGACATGTATGTTATGACATGTGGGACAGGGCAGGTGTGGGGTTGATCAGGGGCGCTGGACCAA GTGAAACCCCAGCGAAGATCCTTAGCTTGGAGTGAGTGGCAGCCTTTGTGGTTATGCTGATGTAGTTCCA GCCTCTAATTTTCTCTTGTTCTTTTGAGTTTGTCAGGATGTGTTGAATCTGTAGACCATTGAACATGGGT AGATTAAGCTGATAACTAATCGATTACCTGAAGTCTTTGAAGTATTCTGTAGTTCAGGTGGGCCTTAAAT ACCACACCTTCTGCCTTAGCCTCCTGAGTGCTGAGATCGCAGGCACTAGCTATTTTATGCTTAGTGTGTG TTCCCTAAAGTCTTTACTATGTGTGATTCCTTCATAACTCAGAAGAGATCTCAAGTCATTGAGCACTGGA TTTTATCTCTTACACCAAATTATAAAAATTAAAGTCCCATAGCTCTAGAGAATAAACCACTATATAAAAT ATTCACTTGATTCTGAAGCCAGCAAGGCTTAAGAAAATAATGCTAGATTTTCTTCTCATTGGTGTCACAG AACTTGGTTGTTGTTCCAAGTCTTCGGTGGTTTTGGCCGTACTGAGCTTACTTCATCAGCCTTCCAGTGT AGGAGACCCGTGCCATTGACCCACAGAGACCCTCTGGGCTATGAGGATTGCTCCTTGTTGCAGGCTCAGA TCCAGGATGAATGAGAGCAGCTCATTGTTGCTACATTTTTTCCCCAACTTGTTGGCAAACAGAATGAAAC AGGAGGATAGATTTATGTATATAGAATGTGAGGACCTTTAATTCCTGTCCTTTCCTGATCTCTGCTCATC ATTTAAGGTGAAAATGTTCCAAGACTTGATGCCTGTGTACTTTTCAATTTGCTGTCTTAGAATATTTTTA CATTTATTTATTCTATTATTTTATGTGTATTGTATACCACATGTGTGCCTGGTACCTGTGAAGGTCAGAA GAGGCCATCAGCTCTACAGAAACTGGAGTTACAGATAGTGAGCTGCCACGTGACTGCTGTGAATTGAACT CATGCTCTGCAAAAGTGATCTTAACTGCTGAGCTAACTCGACTCTCCTGCCCTGTAATTTGCTTTTTAAA AATATTTGTATTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30605060 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30605060 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12076 same embryo
	EMAGE:12075 same embryo
	EMAGE:12079 same embryo
	EMAGE:12074 same embryo
	EMAGE:12078 same embryo
	EurExpress:euxassay_007028 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source