EMAGE mouse gene expression spatial database

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EMAGE:12078

Ddx17 DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 ( MGI:1914290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007031. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007031_01	euxassay_007031_02	euxassay_007031_03	euxassay_007031_04

euxassay_007031_05	euxassay_007031_06	euxassay_007031_07	euxassay_007031_08	euxassay_007031_09

euxassay_007031_10	euxassay_007031_11	euxassay_007031_12	euxassay_007031_13	euxassay_007031_14

euxassay_007031_15	euxassay_007031_16	euxassay_007031_17	euxassay_007031_18	euxassay_007031_19

euxassay_007031_20	euxassay_007031_21	euxassay_007031_22	euxassay_007031_23	euxassay_007031_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12078_wholemount_strong.wlz

12078_wholemount_moderate.wlz

12078_wholemount_weak.wlz

12078_wholemount_possible.wlz

12078_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12078_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

spotted

moderate expression: see section 09 10 11 12 13 15 16 17

hypothalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 15 16 17 18 19 20

telencephalon mantle layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

pons mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

retina

strong

single cell

strong expression: see section 02 03 04 05 24

urinary system

strong

spotted

strong expression: see section 04 05 22 23

metanephros

strong

spotted

strong expression: see section 03 21

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 16 17 18 19 20

male reproductive system

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9452
Entity Detected:	Ddx17, DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 ( MGI:1914290)
Sequence:	sense strand is shown >T9452 GAACCCTACGCCGGTTGCGCACATTGCCTTGTTTAAGGTGGGCCGACCAGGGCCCCCATCTCCGTCACCG ACGTAAAGCGGTTCCGAGAATGTCCGTGGCGTCGCGCGGAGCCCTCCAGACGCTGTTTACGTAGCGTCTG GGCCGTGCGTAGCGTTAAGTTGGAGCGGTCTCCGCGGCCGCCGCCATTTTGTGCAGTTGCTGGGAAGGAA GGGAGACGCCTCAACCGTGGCACCGCCCGGTTTGAGCGGGACCAGACCTCCCGACTGTCCTAACAACCCC GATTCTCTGAGTTGTGCTCCCGTCTCCGACGAGAGAGGCGGCGACGGTGGCGTCTGCGACGGGAGACAGC GCGTCGGAGCGAGACAGCGCCGCGCCTGCCGCCGCCCCAACAGCGGAGGCGCCGCCGCCGCCATCGGTCA TCACTAGACCCGAGCCGCAGGCCCTCCCGAGCTCGGTCATCCGTGCCCCGCTCCCAGATCTTTATCCGTT TGGGACCATGCGTGGAGGCGGCTTTGGGGATCGGGATCGTGACAGGGACCGTGGAGGGTTTGGAGCAAGA GGTGGTAGTGGGCTTCCCCCTAAGAAGTTTGGTAATCCTGGGGAGCGGTTACGAAAAAAGAAGTGGGATT TGAGTGAACTCCCTAAATTTGAGAAGAATTTTTATGTTGAGCATCCAGAAGTAGCAAGACTGACTCCGTA TGAGGTTGATGAGCTACGGCGTAAGAAAGAGATTACAGTGAGAGGGGGAGATGTTTGTCCAAAACCTGTC TTTGCCTTCCATCATGCTAACTTTCCACAGTATGTGATGGATGTGCTGATGGATCAGCACTTTACAGAAC CCACTCCCATTCAGTGCCAGGGATTTCCTTTGGCTCTTAGTGGCAGGGATATGGTTGGCATTGCACAGAC TGGCTCTGGGAAGACATTGGCGTATTTGCTGCCTGCGATTGTTCATATAAACCACCAGCCATACTTGGAA AGAGGAGATGGTCCAATTTGTCTAGTGCTGGCTCCTACCAGAGAGCTTGCGCAGCAGGTGCAGCAGGTGG CTGACGATTATGGAAAATGCTCCAGGTTGAAGAGTACGTGCATTTACGGAGGTGCTCCTAAAGGTCCCCA AATTCGAGACTTGGAAAGAGGTGTTGAGATTTGCATAGCCACTCCTGGGCGCCTAATAGATTTCCTGGAG TCAGGAAAGACAAACCTTCGCCGATGTACTTACCTTGTGTTGGATGAGGCTGACCGGATGCTTGATATGG GCTTTGAGCCCCAGATCCGTAAAATTGTTGATCAAATCAGGCCTGACCGGCAGACACTGATGTGGAGTGC AACCTGGCCGAAGGAAGTGAGGCAGCTTGCAGAGGATTTCCTGCGGGACTACACCCAGATCAATGTGGGC AATCTGGAGCTGAGTGCCAACCACAACATCCTACAGATTGTAGATGTCTGTATGGAGAGTGAAAAAGACC ACAAATTGATCCAGCTGATGGAGGAAATCATGGCGGAAAAGGAAAATAAGACTATAATATTTGTGGAGAC AAAGAGGCGCTGTGATGACCTCACACGAAGAATGCGCAGAGATGGTTGGCCTGCTATGTGTATCCATGGA GACAAGAGTCAACCAGAAAGAGATTGGGTACTTAATGAGTTCCGATCTGGAAAGGCTCCTATCCTCATTG CCACGGATGTAGCCTCCCGTGGGCTAGATGTGGAAGATGTCAAGTTTGTCATCAACTACGATTATCCAAA CAGCTCAGAGGATTATGTTCACCGTATTGGCCGAACGGCCCGCAGCACCAACAAGGGCACTGCCTATACT TTCTTTACCCCGGGCAACCTGAAGCAGGCTAGAGAGCTGATCAAAGTATTGGAAGAGGCCAATCAAGCCA TCAATCCAAAATTGATGCAGCTTGTGGACCACAGAGGTGGCGGCGGAGGAGGGGGAGGCCGCTCACGATA CCGGACTACTTCTTCAGCCAACAATCCCAATCTGATGTATCAGGACGAGTGTGACCGGAGGCTTCGAGGG GTCAAGGATGGTGGCCGGAGAGATTCTACAAGCTACAGGGATCGTAGTGAAACCGATAGAGCCAGTTATG CTAATGGCAGTGGCTATGGAAGCCCAAATTCTGCCTTTGGGGCACAAGCAGGCCAATACACCTATGCTCA AGGCACCTATGGGGCAGCTGCCTATGGCACCAGTGGCTACACGGCGCAGGAGTATGCTGCTGGCACTTAC GGGGCGAGCAGCACTGCCTCAGCAGGGAGGAGCTCTCAGAGCTCCAGCCAGCAGTTTAGTGGGATAGGCC GATCTGGGCAGCAGCCACAGCCACTGATGTCACAGCAGTTTGCACAGCCTCCAGGAGCTACCAATATGAT AGGCTACATGGGGCAGACTGCTTACCAGTACCCTCCCCCTCCCCCTCCCCCTCCTCCATCTCGCAAATGA AACTGCATGGCAGGAGTGACTCGTGCAGATGAATTACACATCTAAAGGAACACTGTCTTTTACCCTTCTA GACCTTTTCATTTTTATTTTTTCTTTTCTCAACCATTACGGTTTATCTTTTTTTAATGCAGATAGTTAAA ATTTCACTGCCAGGTTTCTTCTGCCCACCCAAAAGATCCAGTCTATAATTACAGATTTTGTAAGAAAAAA TATATATACACATAACTGACTGGAAAGAATTAATTTCTTTCCCCCAGCTTAATGCATGTTGAGGATATTG AGCTATTTTTCATCTTAAAGGTATTAGGCACTTTTGTTGGAGCCTGTTTATCCATGGGAGGGTGGGTAGG GAAGGAGGGACTGCTCTGCAGGAAGTGGTATCTGTTCTTTAGACTGCTCTCCTTAGGGAGGAGCCAAGAG GGATTTCCATGCACACTGCCCATGGTGGGTTTAAGTTACTGAAGTTCGATTACTCAGGCCTGATGAATTT TTAAGACTGCTTTGTTGGTCATTTGGCTGATGGCTTCTTATGTGCATTCAACAGTTAAAAATTCCTAATC GATTCTGTCATCATGCCCCCTCCCCACTTGTTCCTGCAGTTATTAAATATTAACCAGGTTAGTGTTGAGT TAGAAATTACATTTCTGAGTTCAAGCGTTGTCTTTTTAAGTTTAAAAAACTATAAAGGGTTGTTTTTACT AGGTAGACTGGCATCTGGGTAGAGGCTGCCTGGGATTTTTATCCCCACATAACCAGGGGCCATCTGAAAG TCATTTTCTAGCCAGATCAGCCACTGAGCGGTAAGCATCAGCGCTCAGCAGAGCCTTCCGAGCAGCATTT AGAAAGCACCTTTGGGAATGATGGACTGCAAGGGAGTGGGCGGATCTTCCTCTTAAACAGTATTCTTAGA GTAGGATTGACCTCATGGGCTGCTCTGCCCACTTGGTCAGGCTCTCCTGAGCAGGACTGCTAAAGAGCTT GCCTCCTCAAAGCCTGGTCTCTGAGGTGCTTTCTAACAGCTGGAGGCACTTGTTCTGGGCTTTCCTTTGC CCTCAGTTTTTCACAGGTAGGTAGTAGCCATGGAGGGCTGAAGCCCAGGCTCTCCTTACTCTCTGGAGTG TGAAGGTAGGGGTGAGCTCGTCTCACCCCTTTATTAAATGCTCCTGGAACTTGGAAGAGCAGCAGATGAA GTTGGAAAGAAAATTCTGCTCTTATCACCTAAACAGGACAGGAGAGCAGATGCCCCAGCGCCTCTAGTAG ATGGTAGTGGTGGGGCTAGTGTGTGGCTGTTACCTGGGCCGCTCAAATTCATTCCAGGGAGGAGGCTCCA CTGCATTCTTTGGCTGTGCCCTGAGGGTGCCTGCTCCTTTGTACGGTAACAGTCAATTTTGAGGTTTCTT TCAGGAAGAGAAAGGGATGGTTTTGAGGGGCTCAGAAAATAGGATTCAGTGTGTAACATAACAGGTAGGT TGTCAGCATGCTTGGCATTCTTCTGTTTTCCTTACCGTTTTACAGACTATGAAGCTGAGGGGGGATGGTA GGCACAGATGTGTGGCAACCCTGCTCTGTGCTCTGAAACCAAAGTGTGTCCTGTCTTGTCTGTCTCTCAC CCTGCCAAAAGCAAGTGTCTAATCACTGATGCTGACCAGCCTGTTACAGATACTTTCTACAACCAAATTC AGATTTATTTTTTATTAAGCATTATACCATGGGCTGTCCTGTGCTCCTGTCACTCTTCAAAAACACCCCT TCTATTACTGCCTCCGGGGATGAAGCAGTAGCTAGGTTGCTTAACCTCCCCTCTCCAGCAGAGGCATAAT CCAGATAAGTAGCAGATGTCATATTTCATAAGCTCTTTTGGCCTGGGTACAGCAGAAAACTAGCAGTTTT CCTACTTGGCGCAAGTGACTTGTGCCTCCTTGTCTGTCCTTTGTCAGCACAGGTGCAGGCAAACCCTTCC AGCTGGGTTAATTGCCGTTTATGGTTTACTCACTTAGGGAAAGGGCTTAGGTGTTAGGCTGTGGGCTGCT TCTCCCTAACCATCCATTGTGCAGACTTCTCATCTAAAAAGGTTGGTGGCTTTTGCTTGGGATCAGTGCT CTGCTAACGCCCTTGCTGGTCTCTCCACACATTCCTGTCATTGAGACTTGAATTGTAGGTGTGATGTTAT GCACAGGATGCTCAGAGCTATGTTACTACTATTCTTAGTTTGTAAATTGTCCTTTTGATACCATCTTGTT TTCTTTTGTAGGTATAAATAAATACTTGACAATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30549388 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30549388 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12076 same embryo
	EMAGE:12075 same embryo
	EMAGE:12079 same embryo
	EMAGE:12074 same embryo
	EMAGE:12077 same embryo
	EurExpress:euxassay_007031 same experiment
	MGI:4824267 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source