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EMAGE:12090

Epyc epiphycan ( MGI:107942)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090
"Pseudo-wholemount" of euxassay_008346. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008346_01 euxassay_008346_02 euxassay_008346_03 euxassay_008346_04
EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090
euxassay_008346_05 euxassay_008346_06 euxassay_008346_07 euxassay_008346_08 euxassay_008346_09
EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090
euxassay_008346_10 euxassay_008346_11 euxassay_008346_12 euxassay_008346_13 euxassay_008346_14
EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090
euxassay_008346_15 euxassay_008346_16 euxassay_008346_17 euxassay_008346_18 euxassay_008346_19
EMAGE:12090 EMAGE:12090 EMAGE:12090 EMAGE:12090
euxassay_008346_20 euxassay_008346_21 euxassay_008346_22 euxassay_008346_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12090Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12090_wholemount_strong.wlz
12090_wholemount_moderate.wlz
12090_wholemount_weak.wlz
12090_wholemount_possible.wlz
12090_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12090_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
humerus
strong strong
regionalstrong expression: see section 03 04 05 21 22 23
foot
strong strong
single cellstrong expression: see section 03 04 moderate expression: see section 02
fibula
strong strong
regionalstrong expression: see section 01 02 03
tibia
strong strong
regionalstrong expression: see section 01 02 03 04
femur
strong strong
regionalstrong expression: see section 01 02 03 07 08 09 10 20 21 22 23
telencephalon meninges
strong strong
regionalstrong expression: see section 01 moderate expression: see section 03 04 05 06 07 08 09 22 23
olfactory lobe
strong strong
regionalstrong expression: see section 02
otic capsule
strong strong
regionalstrong expression: see section 16 17 18 19
eye
strong strong
regionalstrong expression: see section 23
perioptic mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 21 22 23
nasal septum
strong strong
regionalstrong expression: see section 12 13 16 17 18 weak expression: see section 15
axial skeleton cervical region
strong strong
regionalstrong expression: see section 07 09 10 11 12 13 15 16
axial skeleton lumbar region
strong strong
regionalstrong expression: see section 11 12 13 15 17 18
axial skeleton sacral region
strong strong
regionalstrong expression: see section 15
axial skeleton thoracic region
strong strong
regionalstrong expression: see section 09 10 11 12 13 15 16
basioccipital bone
strong strong
regionalstrong expression: see section 08 19
exoccipital bone
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 09 10 16 17 18 19 20 21 22 23
foramen ovale of chondrocranium
strong strong
regionalstrong expression: see section 08
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 03 04 05 06 19 20 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 06 07 08 09 20 21
viscerocranium
strong strong
regionalExpression in the turbinate bone.
scapula
strong strong
regionalstrong expression: see section 01 02 03 04 21 22 23
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 18 19
axial skeleton tail region
strong strong
regionalstrong expression: see section 13 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7190
Entity Detected:Epyc, epiphycan ( MGI:107942)
Sequence:sense strand is shown

>T7190
CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCGGACGCGTGGGTCGACCCACGCGTCCGTTCATCA
AGTCGGAGCCAAAGAAATCTTGAAAGATGGGAATGCTAGCAAGAGTTGCCCTGGGACTTATCATCATTGA
TGCTGTATTGGCTGCACCAACTACAGAGCTCTTTAACTATGACTCAGAAGTGTATGATGCCATCTTGGAG
GACACAGGGACCTTTTATAACTATGAACACATTCCTGACAACCATGTAGAGAACGAGAAAGTGTCTGAGA
GGCTTTCTGGGAACAGAGAGCTCCTGACCCCAGGCCCACAGCTAGGGGACAACCAGGATGAAGACAAGGA
TGAAGAATCTACTCCCAGGCTGATCGATGGCTCTTCCCCACAGGAGCCAGAATTCCCGGGGCTTCTGGGT
CCACACACCAACGAAGACTTTCCAACTTGTCTCCTGTGTACTTGTATAAGTACCACTGTATACTGTGATG
ACCATGAACTGGACGCTATTCCTCCACTGCCCAAGAAGACTACATATTTCTATTCACGTTTTAACAGAAT
TAAAAAGATCAACAAAAATGACTTTGCAAGCTTAAATGATTTAAAAAGGATCGATCTGACATCAAATTTA
ATATCGGAGATTGATGAAGATGCATTCCGAAAGCTGCCTCACCTCCAAGAGCTGGTTCTGCGTGACAATA
AGATAAAGCAGCTTCCAGAATTGCCAAACACTTTGACATTTATTGATATCAGCAACAATAGACTGGGAAG
AAAAGGCATTAAACAAGAAGCTTTTAAAGATATGTATGATCTCCACCATCTCTATATCACCGATAACAGC
TTGGACCACATCCCTCTACCACTCCCAGAAAGTCTGCGGGCTCTTCACCTCCAGAATAATGACATTCTGG
AGATGCATGAAGATACTTTCTGCAATGTTAAAAATTTAACTTATGTTCGTAAGGCATTAGAGGATATTCG
ACTGGATGGAAACCCTATCAACCTCAGCAGAACTCCTCAAGCCTACATGTGTCTACCTCGTTTGCCCATT
GGGAGTTTTATCTAATGTTAGGATGCTAGGCAAATATCAGAATTGTGATGCTTTCTGTAGCCAAGAGAAG
CATATATCTCTTCAGAAGCAAACTCAGTATATTTGAGATGCGTTTAAAACATGTGGCACAATGATATAAA
CAATAAGCTAAAGGTGTTGAGATAAAATAAGAGTTCAATAATTTAATTAAACATGATTAGGTATAAATTA
ACAAAATATATAGACAGTAAGTTAAATAAAATGTATGCCAACTAGTCATCAAATCTCATCAAGAATTGTA
GGTGTTCATGTCAAGTTAAATATGTAAGAAATAAAATATTGGTAATGAATACTTTAGATACGTTGCTAAG
ATGTAGACATTTTAATTAAACTTCTCTAGTCTTTATTTTCACTCATGTATGTTTCCCACCTGATTCAGTT
AAGAGCAGAAAAAAATATTAAATGTGTTTGAAAGATAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4037028 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4037028 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12092 same embryo
 EMAGE:12091 same embryo
 EMAGE:12088 same embryo
 EMAGE:12089 same embryo
 EMAGE:12093 same embryo
 EurExpress:euxassay_008346 same experiment
 MGI:4824605 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS