EMAGE mouse gene expression spatial database

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EMAGE:12139

Rab10 RAB10, member RAS oncogene family ( MGI:105066)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008412. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008412_01	euxassay_008412_02	euxassay_008412_03	euxassay_008412_04

euxassay_008412_05	euxassay_008412_06	euxassay_008412_07	euxassay_008412_08	euxassay_008412_09

euxassay_008412_10	euxassay_008412_11	euxassay_008412_12	euxassay_008412_13	euxassay_008412_14

euxassay_008412_15	euxassay_008412_16	euxassay_008412_17	euxassay_008412_18	euxassay_008412_19

euxassay_008412_20	euxassay_008412_21	euxassay_008412_22	euxassay_008412_23	euxassay_008412_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12139_wholemount_strong.wlz

12139_wholemount_moderate.wlz

12139_wholemount_weak.wlz

12139_wholemount_possible.wlz

12139_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12139_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9332
Entity Detected:	Rab10, RAB10, member RAS oncogene family ( MGI:105066)
Sequence:	sense strand is shown >T9332 GGGAAAAAGGTGGCTCTGGCCGGGGCGGCTCTGTTTCCTGGGGTTATGTAGCAGGGCTTGTCGGCTCGCG AGACCTCCCGCTGCCCTCGCCCGTGTCCTAGCGCGGGGTTTCTGCTCCGGGCGGAGGCCGTGTTCAAGCC CGCCGCGCTCCCTCGACGTAGAGCTCGCTCGTCCGCCCGTGGGAGCGTCCCGGCCGAGCTGCGCTGAGGG GGGAGGGGAGGCCATTTTGTCCCGACCGACTCCCCGGAACCGGGCGGAGCGGCTGGGAGAGGCTGCGGAG CCGCGGGCGCCGCCCCTCGGAGGCACGGGCGCCGCCACCGTCGGGGCTTCCTCGACGAGGCCGTTCGGAA GGTCTCCTGCTCCGTCTCGAGAGCTGCTTTCTCCTTCCGCACACGCTACCCGGCTGCTGCGGCCCCAGAA CGCCCGGGTGAGGAGTTGGTTGTAGTGAGCAGTTCCGATCCCTTGGGGCTACCGGCGGCGAGCGCCCGAG CCGCTCCTCCCAATGGCGAAGAAGACGTACGACCTGCTTTTCAAGCTGCTCCTGATCGGGGACTCGGGAG TGGGCAAGACCTGCGTCCTTTTTCGTTTTTCGGACGATGCCTTCAATACCACCTTTATTTCCACCATAGG AATAGACTTTAAGATCAAAACAGTGGAACTACAAGGAAAGAAGATCAAGCTACAGATATGGGACACAGCA GGCCAGGAGCGATTTCACACCATCACAACCTCCTACTACAGAGGAGCAATGGGCATCATGCTAGTGTATG ACATCACCAACGGTAAAAGCTTTGAGAACATCAGCAAGTGGCTTAGAAACATAGATGAGCATGCCAATGA AGATGTGGAAAGAATGTTACTAGGGAACAAGTGTGACATGGACGACAAGAGAGTTGTACCGAAAGGCAAA GGAGAACAGATTGCAAGGGAGCATGGTATTAGGTTTTTTGAGACTAGTGCAAAAGCAAATATAAACATCG AAAAGGCGTTCCTCACATTAGCTGAAGACATCCTCCGAAAGACCCCTGTAAAAGAACCCAACAGTGAAAA CGTAGATATCAGCAGTGGAGGAGGCGTGACGGGCTGGAAGAGCAAGTGCTGCTGAGTGCTCTCCTGTCCA TCTGCTGCCATCCACCATCCGGTTCTCTTCTTGCTGCAAAATAAAACACTCTGTCCATTTTTAACTCTAA ACAGATATTTTTGTTTCTCATCTTAACTATTCAATCCACCTATTTTATTTGTTCTTTCATCTGTGACTGC TTGCGGACTATTATAATTTTCTTCAAACAAACAAACAAAAATGTATAGAGAAATCATGTCTGTGAGTTCA TTTTGAGATTTACTTGCTCACTCAGCCCTGCACTTCAGTTGTATTATAGTCCAGTTCTTATCAACATTAA ACTAGAGCAATCATTTCAACTATTCTGCAAATTGTATAAGAATAAAAGTTAGAATTAACAATTTTATTTT GTACAACAGTGGGATTTTCTGTCATGAATAATGTGCTTGATCCCTATAATCTATAGACGTGATAGCAAAG AAACAAAAGCCAGGAAAACACTCATTTTCGCCTTGAACTGTATGTGGGTTAATTTGTCCTGTGCCTTATG TGGAAAGGAACGTCTTTGGTTTTCTTTTTTTGTTTTGGTGGAAGCATGAGCAGGAGACATTTCATCCCAG CATAAACTCCTTCAGATGCTTGTGGTTTGCTTGGCTGTATCTTTCACGATAGTTAACTGAGGACAAAGGG TAATGCAGAAGTGATATTTGATTTCCATTCTCATTCCCAGTGGCCTTGATATTTAAACTGATTCCTGCCA CCAGGTCCTTGGGCCACCCTGTCCCTGCGTCTCATCTTTCTGCATGCTGCTTTGTTTGCCCCTACTACTC CTGGTGTGAGTTATTTAAAGATGAAAGGTTTGTCAAGTTTAACATACAGCACTGATTTTACCTTGCTAAG TAAACAGAAAGATAAATTCTAACTGCCTATTAACTGTCAGTTAATTCGGGTCAGCCCCTCATTTACTCTC TCCCCAAACCTGCCCCGCTGTTGGCCTTCTGTCCCCCACCCCTTCCTGTCTATCCATGGAGTTTATGAAA TGGAAGAGTTAATTGCATGCACTAGTGTTTGGAGGGTGTTGTGGCTTGTCTTTCTAATTAGGTATATATC CTATTCACTTTCCTAGAGTAAACCTCCTAACCTGGATTTGACTAGTCTCCATTATGCCAGCTCCTCTGGC AGTTCGGCAGCCCAGGAAAGACTGGTTTTAAAAAAGAGCAAGAAGGAGTGGGTCTTAGTAGCGTTTGTCA AATGTATTGAGATTTGGCCTTTGAAGAACACATTTGGTATTAAGTTGTCTTTACTGTAAGATTTAGAAAT GCATTATAATATTATTAATTTCAAGTCCTATCTTTACATCCTCTCAGAAAACTTCTGATACCATGAATAT TTTAAAAAAGAAAAAGGACCGCAAAAGGCTTCTCGTGTAAAGCTGAGCACTCTGGCTCTGTCTGACCCGC CCTTCTCTCCCCTTTCTAGTTTGGCTTCATTGCTTCTCATTAGGATAGGGTAGGCTAAGTTCCCTATGCT GCTAGCATCCTAAGATGATACCTTTGTGGAAATAATTGTGAATAGCATGATTCATTTCAAGCAGAGGCTG AGTTTAGGACAGCAGCTACCATTGAGAAGATTTCTGTGACATGCATTTTAATGACCTCTTGGCTCACATA AACAATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTGTCCCACAGACAATTCTAC ATGCCTCACTGGTGGCTCTTAGGGTTTCTTCCCTCACTTGACTTTATCATTGTTTACTAGTAAAAAGCAG CATTGCCAAATAACCCCTGATTTTCCACTATGAAAAATGCGATGTGTAAGCTTTTAAAGTTGTAGGTTAA ACCTCCTGTTGTTATCTTTGCTGTTTGTGTTGCTTCCTTCTATCTGGAATGTGGTAGCCACCTCTTTAAA CTTTTTTTAACCCTTAATTCAGTGACTTAAGGTTTGGAGATACACACTGGGATTTTGGCTAACAGACTGA CAGTTTTGTTGTTTGCATAATTATAATCGACATTGTACAGAGAAAGGATAAGGCTACCTTTGTTAAATCT GCACTTTCTGAATATCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6848840 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6848840 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12136 same embryo
	EMAGE:12137 same embryo
	EMAGE:12135 same embryo
	EMAGE:12140 same embryo
	EMAGE:12138 same embryo
	EurExpress:euxassay_008412 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source