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EMAGE:12156

Gja3 gap junction protein, alpha 3 ( MGI:95714)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156
"Pseudo-wholemount" of euxassay_008372. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008372_01 euxassay_008372_02 euxassay_008372_03 euxassay_008372_04
EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156
euxassay_008372_05 euxassay_008372_06 euxassay_008372_07 euxassay_008372_08 euxassay_008372_09
EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156
euxassay_008372_10 euxassay_008372_11 euxassay_008372_12 euxassay_008372_13 euxassay_008372_14
EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156
euxassay_008372_15 euxassay_008372_16 euxassay_008372_17 euxassay_008372_18 euxassay_008372_19
EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156 EMAGE:12156
euxassay_008372_20 euxassay_008372_21 euxassay_008372_22 euxassay_008372_23 euxassay_008372_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12156Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12156_wholemount_strong.wlz
12156_wholemount_moderate.wlz
12156_wholemount_weak.wlz
12156_wholemount_possible.wlz
12156_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12156_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lens
strong strong
homogeneousstrong expression: see section 01 02
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35173
Entity Detected:Gja3, gap junction protein, alpha 3 ( MGI:95714)
Sequence:sense strand is shown

>T35173
CCAACACTGTGGACTGTTTCATCTCCAGGCCCACAGAGAAGACCATCTTTGTCATCTTCATGCTGGCTGT
GGCCTGTGCGTCACTGGTACTCAACATGCTGGAGATTTACCACCTGGGCTGGAAGAAGCTCAAGCAGGGA
GTTACTAACCACTTCAACCCAGATGCCTCAGAAGCCAGGCACAAGCCCTTGGACCCCCTACCCGCGGCCA
CCAGCTCTGGCCCACCCAGCGTCTCCATCGGGTTCCCACCTTATTACACACACCCTGCCTGTCCCACAGT
ACAGGGAAAGGCCATAGGGTTTCCTGGGGCCCCACTATCACCAGCAGACTTCACAGTGGTGACTCTAAAC
GATGCCCAAGGCAGAAACCACCCAGTCAAACACTGCAATGGCCACCACCTGACGACAGAGCAGAACTGGA
CCAGGCAAGTGGCAGAGCAGCAGACTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100074. Forward Primer - name:100074_F_cDNA_Gja3, sequence:CCAACACTGTGGACTGTTTCAT; Reverse Primer - name:100074_N_SP6_cDNA_Gja3, sequence:GAGTCTGCTGCTCTGCCACTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12158 same embryo
 EMAGE:12161 same embryo
 EMAGE:12157 same embryo
 EMAGE:12160 same embryo
 EMAGE:12159 same embryo
 EurExpress:euxassay_008372 same experiment
 MGI:4825056 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS