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EMAGE:12159

Gja8 gap junction protein, alpha 8 ( MGI:99953)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159
"Pseudo-wholemount" of euxassay_008373. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008373_01 euxassay_008373_02 euxassay_008373_03 euxassay_008373_04
EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159
euxassay_008373_05 euxassay_008373_06 euxassay_008373_07 euxassay_008373_08 euxassay_008373_09
EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159
euxassay_008373_10 euxassay_008373_11 euxassay_008373_12 euxassay_008373_13 euxassay_008373_14
EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159 EMAGE:12159
euxassay_008373_15 euxassay_008373_16 euxassay_008373_17 euxassay_008373_18 euxassay_008373_19
EMAGE:12159 EMAGE:12159 EMAGE:12159
euxassay_008373_20 euxassay_008373_21 euxassay_008373_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12159Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12159_wholemount_strong.wlz
12159_wholemount_moderate.wlz
12159_wholemount_weak.wlz
12159_wholemount_possible.wlz
12159_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12159_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lens
strong strong
homogeneousstrong expression: see section 01
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35175
Entity Detected:Gja8, gap junction protein, alpha 8 ( MGI:99953)
Sequence:sense strand is shown

>T35175
TCAGTCGCTTTTGTGTCACTCTTCCTCAACATCATGGAGATGAGCCACCTGGGCATGAAAGGAATCCGGT
CTGCCTTCAAGAGGCCTGTAGAGCAACCACTGGGGGAGATTGCTGAGAAGTCCCTCCACTCCATTGCAGT
TTCCTCCATCCAGAAAGCCAAGGGCTACCAGCTTCTAGAAGAAGAGAAGATCGTATCACACTATTTCCCT
TTGACAGAGGTTGGAATGGTGGAGACCAGCCCTCTTTCGGCCAAGCCTTTTAGTCAGTTTGAGGAGAAGA
TCGGCACAGGACCCCTGGCAGATATGTCACGGAGTTACCAAGAAACCCTGCCTTCTTATGCTCAGGTGGG
GGTCCAGGAAGTGGAGCGGGAAGAGCCGCCTATAGAAGAGGCTGTGGAACCGGAAGTGGGAGAGAAGAAG
CAAGAAGCAGAGAAGGTGGCCCCAGAAGGGCAGGAGACAGTTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97769. Forward Primer - name:097769_F_cDNA_Gja8, sequence:TCAGTCGCTTTTGTGTCACTCT; Reverse Primer - name:097769_N_SP6_cDNA_Gja8, sequence:CAACTGTCTCCTGCCCTTCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12158 same embryo
 EMAGE:12161 same embryo
 EMAGE:12157 same embryo
 EMAGE:12160 same embryo
 EMAGE:12156 same embryo
 EurExpress:euxassay_008373 same experiment
 MGI:4825058 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS