EMAGE mouse gene expression spatial database

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EMAGE:12174

Rxfp2 relaxin/insulin-like family peptide receptor 2 ( MGI:2153463)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008331_01	euxassay_008331_02	euxassay_008331_03	euxassay_008331_04

euxassay_008331_05	euxassay_008331_06	euxassay_008331_07	euxassay_008331_08	euxassay_008331_09

euxassay_008331_10	euxassay_008331_11	euxassay_008331_12	euxassay_008331_13	euxassay_008331_14

euxassay_008331_15	euxassay_008331_16	euxassay_008331_17	euxassay_008331_18	euxassay_008331_19

euxassay_008331_20	euxassay_008331_21	euxassay_008331_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12174_wholemount_strong.wlz

12174_wholemount_moderate.wlz

12174_wholemount_weak.wlz

12174_wholemount_possible.wlz

12174_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12174_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

peritoneal component

strong

regional

strong expression: see section 08 09 10 11 14 15 16 17 18 moderate expression: see section 06 07 19 weak expression: see section 01 02 03 04 05 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35258
Entity Detected:	Rxfp2, relaxin/insulin-like family peptide receptor 2 ( MGI:2153463)
Sequence:	sense strand is shown >T35258 CTCACAGAAGTCCTTTATGGGGTTAAACTCCTTGTTTTTCTTGCCCATGGTGGGTAACCGGCTCGAGGCC CTTCCTGAAACATTGTGTGCTCAGATGCCTCAACTCAACTGGGTGGATCTGGCAAACAATGGAATAAAGT ACATAACGAACTCCACCTTCCTAACGTGCGACTCGCTCACGGTTCTGTTTCTGCCTAGAAATCAAATTGG TTTTGTTCCAGAGAAGACATTTTCTTCATTAAAAAATTTAGGAGAACTGGACCTGTCTAGCAATATGATA ACAAAACTCCCAGTCCACCTTTTCAGCGACCTTCATCTTCTCCAGAAGCTGAACCTGTCATCCAACCCTC TTCTGTATGTCCACAAGAACCAGTTTGGAAGTCTCAAACAACTTCAGTCTCTAGACCTGGAAAGGATAGA GATTCCAAACATAAGCACAGGAATGTTCCAGCCAATGAAGAACCTTTCTCACATTTATTTGAAAACCTTT CGATACTGCTCCTATGTCCCCCATGTCCGAATCTGTATGCCGTCGACTGATGGTATTTCTTCGTCTGAGG ACCTCTTGGCTAACGGTATCCTCAGAGTGTCTGTCTGGGTTATAGCTTTCATTACCTGCGTTGGGAATTT CCTTGTCATAGCCGTGAGATCTCTCATTAAGGCTGAGAATACAACTCACGCTATGTCCATCAAAATCCTT TGTTGTGCCGATTGCCTGATGGGGGTGTACCTGTTCTCCGTGGGCGTCTTTGACATCAAGTACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 92199. Forward Primer - name:092199_F_cDNA_Lgr8, sequence:CTCACAGAAGTCCTTTATGGGG; Reverse Primer - name:092199_N_SP6_cDNA_Lgr8, sequence:GGTACTTGATGTCAAAGACGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12173 same embryo
	EMAGE:12176 same embryo
	EMAGE:12175 same embryo
	EMAGE:12177 same embryo
	EurExpress:euxassay_008331 same experiment
	MGI:4827841 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source