EMAGE mouse gene expression spatial database

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EMAGE:12216

Fbxl12 F-box and leucine-rich repeat protein 12 ( MGI:1354738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008307. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008307_01	euxassay_008307_02	euxassay_008307_03	euxassay_008307_04

euxassay_008307_05	euxassay_008307_06	euxassay_008307_07	euxassay_008307_08	euxassay_008307_09

euxassay_008307_10	euxassay_008307_11	euxassay_008307_12	euxassay_008307_13	euxassay_008307_14

euxassay_008307_15	euxassay_008307_16	euxassay_008307_17	euxassay_008307_18	euxassay_008307_19

euxassay_008307_20	euxassay_008307_21	euxassay_008307_22	euxassay_008307_23	euxassay_008307_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12216_wholemount_strong.wlz

12216_wholemount_moderate.wlz

12216_wholemount_weak.wlz

12216_wholemount_possible.wlz

12216_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12216_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7207
Entity Detected:	Fbxl12, F-box and leucine-rich repeat protein 12 ( MGI:1354738)
Sequence:	sense strand is shown >T7207 CTTCAGCCTTCTGGATTTGCTGTTAGTCTTCCTCTTGGCCCTGGCCACGCCTCGTGCCCCCTCCTCCACC AAGCCACATCCAGGTTTTCCTAGAGTCACGCCTTCTGGGTTGTCTGCGGACCCCTTTTGGGTTTGCTGCT TATACTTAGCTCCACCTTATGGCCACGCCCCCGAGGCACTCGTCACCTCCAGCCTTCTGGGTTTGCTGTT CGTCTTCCTCTTGGCCCTGGCCACGCCTCGTGCCCCCTCCTCTCCCAAGCCACATCCAGGTTTTCCTATT TCCAGTTCCATCCGCTAGCCAAGCCAGTCAGGCCTAGTCAGTCTCTAGTCTTGACCTCTAAACGGTCGTC AGCCCGCGCCTGTCCAGTCCCGGGGCCTCGGGCACTTTCCTAGAAAAGTAGGGATTCTCGGCGGGCCTCT GAAAGTCGCGCCGGTTTGGCCAGTCCGACCACCCCAGCGCTGTCCAGGGAACGCGGCCTCGCCGCCACCG GAAACGGAAGCGTGCGACAGCTGGACCATGGCGACTCTGTTTGACCTCCCAGACTTGGTGCTCTTGGAGA TCTTCTCTTACCTCCCTGTCCGGGACCGGATCCGCATCTCCAGGGTCTGTCACCGCTGGAAGAGGCTAGT GGATGACCGGTGGCTATGGCGACACGTCGACCTGACGCTCTACACGATGCGGCCGAAAGTCATGTGGCAC CTCCTGCGCCGGTACATGGCGTCCCGGCTCTACTCGTTGCGCATGGGCGGCTACCTGTTTTCTGGCTCTC AGGCCCCACAGCTGTCCCCCGCCTTGATGAGGGCCCTGGGTCAGAAATGCCCCAATCTGAAGCGCCTGTG CCTGCACGTGGCTGACCTGAGCATGGTGCCTATCACCAGCCTTCCTAGCACACTGAGGACCCTGGAGCTG CACAGCTGCGAAATCTCCATGATCTGGTTGCAGAAAGAGCAGGACCCCACGGTGCTGCCTCTTCTGGAAT GCATCGTGCTGGACCGAGTGCCGGCCTTCCGCGATGAGCATCTGCAGGGCCTTACCCGATTCCGAGCCCT GCGCTCGCTGGTGCTGGGCGGCACCTACCGGGTCACTGAGACCGGGCTAGATGCCAGCCTGCAGGAGCTC AGCTACCTGCAAAGGCTTGAGGTGCTGGGCTGCACCCTGTCAGCTGACAGCACGCTGCTGGCCATCAGCC GCCACCTTCGAGATGTGCGCAAGATTCGGCTGACCGTTGGGGGCCTCTCAGCCCAGGGCCTGGTCTTCCT GGAGGGAATGCCTGTCCTGGAGAGTTTGTGCTTCCAGGGTCCCCTTATCACCCCAGACATGCCCACACCC ACTCAGATTGTGTCCTCCTGCCTCACCATGCCGAAGCTCAGAGTGCTTGAGGTGCAAGGGCTGGGCTGGG AGGGTCAGGAAGCAGAGAAAATCCTGTGCAAGGGCCTGCCCCACTGCATAGTTATTGTTAGGGCTTGTCC CAAAGAATCCATGGATTGGTGGATGTGAGGGCATCACCCATCCATCTCTGGAACCTAGCCTAGCTTGGAG AGTGACCTTATAAGCAAACTTAAGCATGTAAATTCATCAGGCGTGAGGGAACTGGAGGTGTCAAGGAAAG GAAACTAGGACCTTGGGGCTTCCTGAGCTGGGGTTATCTGTAATCTGGGTCACCTCAGACTTGTCATCAA GCCTCTGGAAGACCCCGTTCACACATTTAGGAATAGGCATCATAGCTACCTCATGATTCATTTTAAAATC TTTAACACCCCCTTCCCCTCTTTCTTTTCCAGTACCTGGGACTCAAACCCATGGCCTTAGGTCTGGTAGA GAAGCACTGCTGTGCTGCTGAGCCACCCCAGAGACCTTAGAGAGGGCTCATTTTCAAGTGGAGGTGTGTG GAGTTCAGGGCTCTGAGGTTGGCCTGGTTTTCTACATACATGGTTGAGAGGCATAGAGGGCAGGGGCAAG GGAGCATGCTGTTGGATTGTCCTAGTTTTCTAGAAGGTTCTCAGTCATATTTACTGAATTTTGTATCCTG TCTTTTTCAAGTTTTCCACAGCATATATTTTACTATTAAAGAAAAATAATGTTTTTCAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4039251 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4039251 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12217 same embryo
	EMAGE:12219 same embryo
	EMAGE:12218 same embryo
	EMAGE:12220 same embryo
	EMAGE:12215 same embryo
	EurExpress:euxassay_008307 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source