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EMAGE:12261

Lgi3 leucine-rich repeat LGI family, member 3 ( MGI:2182619)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261
"Pseudo-wholemount" of euxassay_007015. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007015_01 euxassay_007015_02 euxassay_007015_03 euxassay_007015_04
EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261
euxassay_007015_05 euxassay_007015_06 euxassay_007015_07 euxassay_007015_08 euxassay_007015_09
EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261
euxassay_007015_10 euxassay_007015_11 euxassay_007015_12 euxassay_007015_13 euxassay_007015_14
EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261
euxassay_007015_15 euxassay_007015_16 euxassay_007015_17 euxassay_007015_18 euxassay_007015_19
EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261 EMAGE:12261
euxassay_007015_20 euxassay_007015_21 euxassay_007015_22 euxassay_007015_23 euxassay_007015_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12261Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12261_wholemount_strong.wlz
12261_wholemount_moderate.wlz
12261_wholemount_weak.wlz
12261_wholemount_possible.wlz
12261_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12261_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hand
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06
foot
moderate moderate
regionalmoderate expression: see section 03 04 05 06 24
diencephalon lateral wall mantle layer
moderate moderate
spottedmoderate expression: see section 11 15 16
telencephalon mantle layer
moderate moderate
spottedmoderate expression: see section 02 03 04 05 24
medulla oblongata basal plate mantle layer
strong strong
spottedstrong expression: see section 14 15 16 17 moderate expression: see section 07 08 09 10 11 12 18 19 20
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 13
pons mantle layer
strong strong
spottedstrong expression: see section 14 15 16 17 moderate expression: see section 05 06 07 08 09 10 11 12 18 19 20
pons ventricular layer
strong strong
regionalstrong expression: see section 13 14
midbrain ventricular layer
strong strong
regionalstrong expression: see section 12 13 14
trigeminal v ganglion
moderate moderate
spottedmoderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24
basal columns
strong strong
regionalstrong expression: see section 11 12 13 14 15 16 17 18 19
stomach mesentery
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12
rest of midgut mesentery
moderate moderate
regionalmoderate expression: see section 16 17 18 19 20 21
urogenital mesentery
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 24 weak expression: see section 23
left lung
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15
right lung
strong strong
regionalstrong expression: see section 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8407
Entity Detected:Lgi3, leucine-rich repeat LGI family, member 3 ( MGI:2182619)
Sequence:sense strand is shown

>T8407
TGTTGCGGCAGCCCGGAGGTGGAAGCTCTCGAGAGGGGCAGAACAGCCTGCAGGGAGCAGCGGGGCGCCG
GCAGGCTGGTGTGGGGGGAGACCCTGCAGGAGTCATGGCCGGGCTACGAGCCAGGCGGGGCCCTGGGCGC
AGGCTACTGGTGTTGTCCACGCTGGGTTTTTGCCTAATGCTGCAAGTCAGTGCCAAGAGACCTCCTAAGA
CGCCCCCCTGCCCACCCAGCTGCTCCTGCACCAGGGACACCGCCTTCTGCGTGGACTCTAAGTCGGTGCC
CAAGAACCTGCCTTCAGAGGTCATCTCCCTGACGCTGGTGAATGCTGCCTTCTCAGAGATCCAGGATGGA
GCCTTCTCCCATCTGCCGTTGTTGCAGTTCTTGTTACTCAACTCTAACAAGTTCACACTGATTGGAGACA
ATGCCTTCATAGGACTGTCGCACCTGCAGTACCTCTTCATAGAGAACAATGACATCTGGGCTCTCTCCAA
GTTTACCTTCAGAGGACTGAAGTCCTTGACACACCTTTCTCTGGCCAACAATAACCTGCAGACACTGCCC
AGAGACATCTTCCGTCCCCTGGACATCCTGAGTGACTTAGACCTCCGGGGCAACGCACTTAACTGTGACT
GCAAGGTAAAGTGGCTGGTGGAGTGGCTGGCACACACCAACACCACGGTGGCTCCCATCTACTGCGCCAG
CCCGCCCCGCTTCCAGGAACACAAGGTGCAGGACTTGCCTCTTCGAGAATTCGACTGCATCACCACTGAT
TTTGTCCTCTACCAGACCCTGTCCTTCCCAGCAGTGTCTGCGGAACCTTTCCTTTACTCTAGTGACCTCT
ACTTGGCTCTGGCCCAGCCAGGTGCCAGCGCCTGCACCATCCTCAAGTGGGACTATGTGGAACGACAGCT
TCGAGACTATGACAGAATTCCAGCCCCTTCAGCTGTGCACTGCAAGCCGATGGTGGTGGATGGCCAGCTC
TATGTCGTTGTGGCCCAGCTGTTTGGTGGCTCATACATTTACCACTGGGACCCCAACACCACACGTTTCA
CCAAGTTGCAGGACATCGACCCACAGCGCGTGCGCAAACCCAATGACCTGGAAGCCTTCCGCATCGACGG
TGACTGGTTTTTCGCAGTGGCTGACAGCTCCAAGGCAGGTGCCACCAGCCTCTACCGTTGGCACCAGAAT
GGCTTCTATTCCCACCAGGCCCTGCACGCCTGGCACCGCGACACCGACCTGGAGTTTGTGGACGGTGAGG
GCAAACCACGGTTAATTGTGTCTAGCAGCTCGCAAGCACCTGTCATCTATCAATGGAGTCGTTCTCAGAA
ACAGTTTGTGGCTCAGGGAGAGGTGACTCAGGTACCTGATGCCCAGGCCGTGAAACACTTTCGTGCTGGC
CGAGACAGCTACCTGTGCCTTAGCCGCTACATTGGCGACTCCAAGATCCTGCGCTGGGAAGGTACCCGTT
TCTCTGAGGTGCAAGCCCTTCCCTCCCGAGGCTCGCTGGCCCTACAGCCCTTCCTGGTGGGTGGTCACCG
CTACCTGGCCCTGGGCAGTGACTTCTCCTTCACCCAGATCTACCAGTGGGATGAGGGGCGACAGAAATTT
GTACGGTTCCAAGAGCTGGCAGTACAGGCACCCCGGGCCTTCTGCTACATGCCTGCTGGAGATGCCCAGC
TGCTCCTGGCCCCCAGTTTCAAGGGACAAACACTGGTGTACCGACATGTTGTGGTGGACCTTAGTGCCTA
GAGGGGGGCTAAGTCCTTTGGGTTCCTCAGGGTGGCACTGTACGATGGGTGGGGACCTCTGTGAGCAACT
TGTGGCCCCAAGTGAAGTCACAGGTGGCCAGCCTGTCTATGTAGCTACACACACATACACAAAGAAGTGG
GCCTGGGCACACACCAGGGACTAGCCCAGGGGAGCCATTCCCATTACTGTAATAAGCACCTACGTATGCA
CCAGGCACCCAGTGTCCAGGCTCTCTATGTGCGCACCAACAGCCCCTGATCTTCAGTGTTCCCTGCTCTG
CTCCGTTGCTAGCGTGTCTGAGTAACAAAGCGAGTGCAGGAAGGGGTGAGCCACAGCCTTCTCTTCTCAG
CTTAGCTTTCTGCTCTCTGGCCTCTGGATGCCCGAGGTGCTTGTGTACCTGTTTGGCATCCACAGTTGCA
GCCCCCCTGCAGGTGTGCCCACACTTCCAAGTGTGCCTGCCCACGCCATGCTCCTTCTGCATGCACCTGT
GGACACTGATGGACAAACTCAGCTGCATACTTGTGCATACCCCACCACACTTCTGATTCTGTATCTGCAC
ACGTCTGGGGGTGTGTACGTGAGCGGACTAGGTGCTGGGCAAACCGGCGTCCTTGATACCCCGTAGCTGC
TCTTCTTGGAGGGTGCCTACAGCACCCACACTTTCTCTGCTCTCCCCAGTGTGCCCCCTGTGTGGCTCAT
AAGGATAGCAACAGAAAACGTGACCATGGCCTCGTGGGGACAAAGCCACTTCATCCAGAAGCAATAATAA
CAAGAGGCAGCAGCAACTGGGTTCTTCACAGTGCAGTATAGCTCTCCTCACTAGAGTTTTCTTCCACGAC
TCAGTCCCAGCTCAAAGGGACACGGGTGGGAAGGCTTTCTCTGGATAGCTATTGCTTTTTCAAGCTCGGG
CCATCTCCTCTCTCACCCTATCTTTGAGATGCGGATCCCAAAGTGTGATTGCTCCTTGACTGGATATATA
AAAAAAAATCAGCCAAGGCAAGAATCCTGAGACAGCCTGAGCTACTTGTGTGGGCGCCAGGAACATGTTG
GTCGCCCTGGCACCCCTCCATGCCATGGAGATGCCCCCCGGAGGCACAGCTCGGTGGAGGCTGGATGAGG
ATGGCAGGGAGGGACACCTGGGAACCAGGCCTCTGCTGCCCTCTGCTGTCCTCTGGGAGCAGCAGCCCTG
AAGTAGGAAATGATCAGGCTGAGAGGATTTCTTGCGGCCTGATGCACCTGGGGATAATGCTAAGATTCCT
TTGCTTCGAATAAAGTTCCACCCCACTGCTAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5686778 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686778 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12260 same embryo
 EMAGE:12263 same embryo
 EMAGE:12265 same embryo
 EMAGE:12264 same embryo
 EMAGE:12262 same embryo
 EurExpress:euxassay_007015 same experiment
 MGI:4825913 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS