EMAGE mouse gene expression spatial database

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EMAGE:12344

Nudt3 nudix (nucleotide diphosphate linked moiety X)-type motif 3 ( MGI:1928484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007084. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007084_01	euxassay_007084_02	euxassay_007084_03	euxassay_007084_04

euxassay_007084_05	euxassay_007084_06	euxassay_007084_07	euxassay_007084_08	euxassay_007084_09

euxassay_007084_10	euxassay_007084_11	euxassay_007084_12	euxassay_007084_13	euxassay_007084_14

euxassay_007084_15	euxassay_007084_16	euxassay_007084_17	euxassay_007084_18	euxassay_007084_19

euxassay_007084_20	euxassay_007084_21	euxassay_007084_22	euxassay_007084_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12344_wholemount_strong.wlz

12344_wholemount_moderate.wlz

12344_wholemount_weak.wlz

12344_wholemount_possible.wlz

12344_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12344_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9190
Entity Detected:	Nudt3, nudix (nucleotide diphosphate linked moiety X)-type motif 3 ( MGI:1928484)
Sequence:	sense strand is shown >T9190 TCCAAAATGGCGGCGCGGTCGTCGTCGGGGGCGGCGGCGGTAGAGGGGGCGGTGGCGGTGGCGACGGCGG CCGTGACGGCGGCGCAGGGCCTGGGCCGGGGGCAATGAGGCTGTCCCGCCGCGGAGCCGTGTAGCGGACA CACAGACCCTCCTCCCCGCCCTCTCGATCGAGCCTGGGGCGCGCGCGCGCGCGGCCGCCCGCCTGCAGCA GGCTCCCCTTCCCTGGACTCCGAGGCCCCGCACGGCACCCCCCACCCGGGCCCGGAGGATGATGAAGCTC AAGTCGAACCAGACCCGCACCTACGACGGCGACGGCTACAAGAAGCGGGCCGCGTGCCTGTGCTTCCGCA GCGAGAGCGAGGAGGAGGTGCTGCTTGTGAGCAGTAGCCGCCATCCAGACCGATGGATTGTCCCCGGAGG AGGCATGGAGCCTGAGGAGGAGCCAAGTGTGGCAGCTGTCCGGGAAGTGTGTGAGGAGGCTGGAGTGAAA GGGACACTGGGAAGACTAGTTGGAATTTTTGAGAACCAGGAGAGGAAGCACAGGACCTACGTGTATGTGC TCATCGTCACGGAGGTGCTGGAAGACTGGGAGGACTCGGTGAACATCGGAAGGAAGAGAGAGTGGTTTAA AATAGAAGACGCCATAAAGGTGCTGCAGTGTCACAAGCCCGTGCAGGCGTCATACTTTGAGACGTTGAGG CAAGGCTACCCTGCCAACAACGGCACCCCTGTGGTGCCCACCACCTACTCCAGCTCCGTGTCAGGCATCA GATGACTGCAGACTTCTGTAAGAGCCGGAGAGTGGAAAGCAGACTGAAGCATGGCTCTCCTCACCACCCC TCACCTCACGGCCTTCTCTGTCACCCAGGCATGGCGGGCAGTGGAGAGGGGACACTGAGGTGCTGCTGTG GGCTGTGGCGGGAGGCGGGTGGGTGTGGCAGGGTGGGAACTCTTTTTTCTCTTCCTGAGGGTGGGGCTGG GTGTAACTCACAAGCACTTTCCTGCATGCCCGATCCTCCCCGCTCGCTCACCCTGCCCTGAGAGGGCACC TCCCTCTCCTTCTTGGGTTCCGAAGCACTCCCATTTGTCTCGTCCCGGCCTGGCCAGATATTTCCTCTGA ATTTTTTTTTTTTTTTCATTAAAAGACACCCATGTGCGATGAAACTTTCTAAAAATCTGTCCTGTGCTGT GCTGTTGAGTCCCTTAGGTTGACCACGCTCCCTTGGGACGCACCTGTCCACACATTGCACACTGTCACGG GACACGTAAATAAGCGGCGTAGAACGAGGAGCTCAACTGTTCAGGTTCTCAAAGTTTTGGGTTTTTTTTT TCCTTTTTTTTTCTCAGATATTTCTGGTTTGAAGCCCCTCACTCTTCACATTTGTTTAATGCTAGTAACT TGGCGGGCTTCCCTGTCCTCTGTTCCCAGCCATGCCGAGCCCGGCCACCTTGCTCTCACTCCTGGGACCC TGGCCTTCTGTCTCTAGGCTATTCCTGTGCCAAGGAAAACAGAAGGGAACCTGTTTTCTTTCTGTTACGA AGCCTGGAAAACAGTGAGAGAAACTGAATTCTGTCCGATAGGCTCACAGTAGGCTTTAAACTGTCACCCT CAATGTCGCAGAAACCGGGCACTGTGTCTGTCCTGCTGGCTGGAGATTCTTTCTGTAATGCTATTTATTG CTGACATAGCCTCTCTTACCCTTATATGCCTTCCACCATGCTGACCTGGCTGTGGCCCCACGCTCTGCAG CCATTCTGCATAGTCCTGTCCCCACGCCGAGAGGCCCCACGACGTCCTGAGAATACATGGGGAGCAGTGT GAGCATGTATGTGCCAAGGGGGGACCAAGAGCTTGCTCAAAAGGGACATTAGCTTTCTGTCTGCTCGGTG TGAGAGGTCATCTGGAAGAGGGGCCCACCGGTGACCCTCTGGCTCCGGTTGGTATTTTATAGAGGTTTTG TGCCGAACTCCCCAGCCAGTTGTGGTGGTTAGTCGGGAAGTAAAGGGTCCTGGGAGGGGCCTTGAGCAAG GAGGGCAAGAGGGGAGCTAGGCCTGGCTTAGCGTGGGTGCTTGGGACTCTTTGTGGCATTGCCTATGTTG AAACGTAGCCAAGATGTGAATTGAAAATAGTACTTCTCTTTATTTAAAAACTAATAAATACTCAAACTTT GAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829170 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829170 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12345 same embryo
	EMAGE:12343 same embryo
	EMAGE:12348 same embryo
	EMAGE:12346 same embryo
	EMAGE:12347 same embryo
	EurExpress:euxassay_007084 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source