EMAGE mouse gene expression spatial database

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EMAGE:12367

Pde2a phosphodiesterase 2A, cGMP-stimulated ( MGI:2446107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007019_01	euxassay_007019_02	euxassay_007019_03	euxassay_007019_04	euxassay_007019_05

euxassay_007019_06	euxassay_007019_07	euxassay_007019_08	euxassay_007019_09	euxassay_007019_10

euxassay_007019_11	euxassay_007019_12	euxassay_007019_13	euxassay_007019_14	euxassay_007019_15

euxassay_007019_16	euxassay_007019_17	euxassay_007019_18	euxassay_007019_19	euxassay_007019_20

euxassay_007019_21	euxassay_007019_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12367_wholemount_strong.wlz

12367_wholemount_moderate.wlz

12367_wholemount_weak.wlz

12367_wholemount_possible.wlz

12367_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12367_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9434
Entity Detected:	Pde2a, phosphodiesterase 2A, cGMP-stimulated ( MGI:2446107)
Sequence:	sense strand is shown >T9434 AGCAGCAGAGGCAGTAGCAGCCGGAGAGGCAGGCGGAGCAGCCCAGGCAGCAGAGCTGGATCGTGTGTTG AGTACAGGCAGAGTCAAGAACCAGCCATTGCTCTTGGTTCCTATGCCTGCTGGGAGTGCCCCAGTCAGAA CTCTGGGCAGTGGGGACAGGGGGGACGGGGCGAGGTGCCACCTTAGTCTGGCTGGGGAGGCAGATGATGA GGAGTGATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGG CTGAGCCGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAGCCGCCGCCGCAGCCATGCGCCGACAGCCT GCAGGATGCTTTGCTGAGCCTAGGCGCCGTTATCGACATTGCTGGCCTGCGACAGGCTGCCAGGGATGCC CTCTCAGCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGCTAGATGGGGAGTCCAGACTGGTGT GTGAGGACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAAGCTGTGATCTCTCAGAAGCGGCT GAGTTGCAATGGGCTGGGACCTTCGGACCTACTGGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCT CCTGACATGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAAGTGTGGCAGCTGTCATCTTGG TGCACTGTGGCCAGCTGAGTGACAGTGAGGAACAGAGCTTGCAGGTGGTAGAGAAGCACGCTCTGGTAGC CCTGCGGAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACGTCAGTGGATGCCTCT GAAGATCAAAAGGATGAGAAGGGGTACACCGACCATGACCGAAAGATCCTGCAACTGTGTGGGGAACTCT TTGACTTGGATGCTACTTCTCTGCAGCTCAAAGTCCTTCAATATCTGCAGCAGGAGACACAGGCCACTCA CTGCTGCCTCCTGCTGGTGTCGGAGGACAATCTACAGCTTTCCTGCAAGGTCATTGGAGACAAAGTGCTG GGAGAAGAGGTCAGCTTTCCATTGACCATGGGACGTCTGGGGCAGGTGGTGGAAGACAAGCAGTGTATCC AGTTGAAGGACCTAACCTCTGACGATGTGCAACAGCTGCAAAACATGTTGGGTTGTGAGCTGCAGGCTAT GCTGTGTGTCCCTGTCATCAGCCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTT GGAGGAGATTTCTTCACAGATGAGGATGAACACGTGATCCAACACTGCTTCCACTACACAGGCACGGTGC TCACCAGCACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGC AAAGAACCTCTTCACCCACCTGGATGATGTCTCTGTCCTGCTACAGGAGATCATCACGGAAGCCAGAAAC CTCAGCAACGCAGAGATCTGCTCGGTGTTCCTGCTGGATCAGAACGAGCTGGTGGCCAAGGTGTTCGATG GTGGCGTTGTGGACGATGAGAGTTATGAGATCCGCATCCCGGCGGACCAAGGCATCGCGGGCCACGTGGC GACTACGGGCCAGATCCTGAACATCCCAGATGCATATGCCCATCCGCTTTTCTATCGCGGCGTAGATGAC AGCACTGGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAGGAGGTCATTGGTG TGGCTGAGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAGTTTGATGAGGACCTGGCCACAGCCTT CTCCATCTACTGTGGCATCAGCATCGCCCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAATACCGC AGCCACCTGGCCAATGAGATGATGATGTATCATATGAAGGTCTCTGATGATGAATACACCAAGCTTCTCC ACGATGGCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTTACCTACACGCCTCGGTCTCT GCCTGAGGACGACACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAA ATTGACTGCCCAACTCTGGCCCGATTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCACCCTACCACA ACTGGATGCACGCCTTCTCTGTCTCTCATTTTTGCTACCTGCTCTACAAGAATCTGGAGCTCTCCAACTA CCTCGAGGACATCGAGATCTTTGCATTGTTTATTTCCTGCATGTGTCATGACCTGGACCACAGAGGCACA AACAACTCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCCGCACTCTACAGCTCAGAGGGCTCTGTCATGG AGAGGCACCACTTTGCTCAAGCCATTGCTATCCTCAACACCCACGGCTGCAATATCTTTGACCACTTCTC TCGGAAGGACTATCAGCGCATGCTGGACCTGATGAGGGACATCATCTTGGCTACAGACCTGGCACACCAC CTCCGCATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAGGCAACACCACA GGCTTCTTCTGTGCCTCCTCATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAG AAAGATTGCAGAGCTGATCTACAAAGAGTTCTTCTCCCAGGGAGACTTGGAGAAGGCCATGGGCAACCGA CCGATGGAGATGATGGACCGTGAGAAGGCCTACATCCCTGAGCTTCAGATCAGCTTTATGGAGCACATTG CCATGCCTATCTACAAGCTTTTACAAGACCTGTTCCCCAAGGCGGCAGAGCTGTATGAACGTGTGGCCTC CAACCGTGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTG GATTTCCTGGACGAGGAATACGAGGTCCCCGATTTGGACGGCACCAGAGCTCCTGTCAATGGCTGCTGCA GCCTCGAGGGCTGAGGGATCCCCTCTAGGGACCCTCCCTGCCTGGCCCTCCTTTCACAACTCTCTACCAG TCTGTTTGATGCATTGGGAACCAGAGCCATGGGTCCTGGGTTCTAGGCCAGGATGTCCCCTGTGACCCGG CACATGCCACCCTTCCTGGGCTTCTTCATCTTTCTCATCTGCAGAAGGCAAGCAAGATTACCAGTCCCAC ACAGACTTTGCCATTTGTACACAAGAGCACAGTAGTGACCAGGGGCAGTGGTATGGGTCTCTTCTGCACC TCTGACTATCCATGCCCAGTCCTGCCCAAGCCGCTCCATCTCTTAAGCAGCGTCACAGAACGTCCTGTGA CCCCGTTAGAGCCCTACTAGGTCAGTACCCTTGTTTTCCTGGAGGAATCCCAGGTCTCTGGAGACTCCAC GATCCTCACCTCATGGAAGGAGGAGAGACTGCTGATCGAGCAGAGGATATAAAACAAGTCACCAGCTGGA AGTACCCTGAGGGTGCCTCTGTGCTTTTGGTAGGATTGGGACCCTTGGAGGAAGCTAACTGCCAGGTTGC TCAGTGCTCTGCAGGAGGAAGTGCTCCAGCAGAGAGGCTGGGAATAATGGGCAGGAAGAATTGTCAAGCC AGGAGACAGTCCTGTACACACCCACACCCTTCAGGCAGTGTGACCTGAAGCAAGTCCCTTTCTCTAGCTA GGCAAGACAGAATCATTAGACAGCCTAGTCTCTCTTCAGATCAAAGTCTCCATAGTTGTGTGGAGTCCGT GAGTGGCCGGGCATGCTCTGCTGCCCTAAGGCACAGAGAGCCTAACTTTTCTTTCCTCTGAGTGTATCCC CTTGGTCTGAGCCCCTCTCCCATTCCTGCCACCAGAGAGATGCATTTTTTCTAGGACTCTGTGCACCTGT TGTGTGTGGTCCCGTGGGCTGAGTGCTTTGTACATGAGAATAAATCTATTTCTTTCTAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30543765 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30543765 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12368 same embryo
	EMAGE:12370 same embryo
	EMAGE:12371 same embryo
	EMAGE:12366 same embryo
	EMAGE:12369 same embryo
	EurExpress:euxassay_007019 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source