EMAGE mouse gene expression spatial database

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EMAGE:12371

Ldb2 LIM domain binding 2 ( MGI:894670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007014. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007014_01	euxassay_007014_02	euxassay_007014_03	euxassay_007014_04

euxassay_007014_05	euxassay_007014_06	euxassay_007014_07	euxassay_007014_08	euxassay_007014_09

euxassay_007014_10	euxassay_007014_11	euxassay_007014_12	euxassay_007014_13	euxassay_007014_14

euxassay_007014_15	euxassay_007014_16	euxassay_007014_17	euxassay_007014_18	euxassay_007014_19

euxassay_007014_20	euxassay_007014_21	euxassay_007014_22	euxassay_007014_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12371_wholemount_strong.wlz

12371_wholemount_moderate.wlz

12371_wholemount_weak.wlz

12371_wholemount_possible.wlz

12371_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12371_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9425
Entity Detected:	Ldb2, LIM domain binding 2 ( MGI:894670)
Sequence:	sense strand is shown >T9425 TGCCTGCCTGCCTGCCTCTGTGTGTGTCTGTGTGTGTGTGAGCGTGTGTGCGTGCGTCTACTTTGTACTG TGAAGAACACAGCCCATGTGCTCTGCATGGACGTTCCTGATACTCTATTAAGCTTGATTTTCGACAAGCA GGCAAGATGTCCAGCACACCACATGACCCCTTCTATTCTTCTCCTTTCGGCCCATTTTATAGGAGACATA CACCATACATGGTACAGCCAGAGTACCGAATCTATGAGATGAACAAGAGACTGCAGTCTCGTACAGAGGA CAGTGACAACCTCTGGTGGGATGCGTTTGCCACTGAATTTTTTGAAGATGATGCCACATTAACACTTTCA TTTTGTTTGGAAGACGGACCAAAGCGATACACTATCGGCAGGACCCTCATTCCCCGTTATTTTAGCACCG TATTTGAAGGAGGGGTGACAGACCTGTACTACATCCTTAAACACTCAAAGGAGTCATATCACAACTCATC CATCACAGTAGACTGCGACCAGTGTGCCATGGTCACACAGCATGGGAAGCCCATGTTTACCAAGGTATGC ACAGAAGGCAGGCTGATCTTGGAATTCACATTTGATGACCTCATGAGAATAAAAACGTGGCACTTTACCA TTAGACAGTACAGGGAGCTGGTCCCCAGAAGCATACTAGCCATGCACGCACAAGATCCTCAGGTCCTGGA TCAGCTGTCCAAAAATATCACCAGGATGGGTCTCACAAACTTCACCCTCAACTACCTCAGGTTGTGTGTA ATACTGGAGCCAATGCAGGAACTGATGTCGAGACACAAAACCTACAACCTCAGTCCCCGAGACTGCCTGA AGACCTGTCTGTTTCAGAAGTGGCAGCGGATGGTGGCCCCGCCAGCAGAACCCACAAGGCAACCGACAAC CAAAAGGAGAAAACGAAAAAACTCCACAAGCAGCACGTCCAATAGCAGTGCCGGGAACACGACCAACAGT GCCGGCAGCAAGAAGAAGACCCCGGCGGCAAGCCTGAGCCTGGCCACTCAGGTACCTGATGTGATGGTGG TAGGAGAGCCAACTCTGATGGGAGGTGAGTTTGGGGACGAGGACGAAAGGCTAATCACTAGATTAGAAAA CACGCAGTATGATGCGGCCAACGGCATGGACGACGAAGAAGACTTCAACAATTCACCTGCCCTGGGGAAC AACAGCCCGTGGAACAGTAAACCTCCCGCCACGCAAGAGACCAAATCAGAAAACGCCCCACCCCAGGCTT CCCAGTAAGATGGGCTACTGGCACCTGATTACGCTGTCCATAGGCCCGTGGGGTAGAATTCCATTCGCAA ATCTCTACTATCAGGAGAGAAAAGAGATGAAATTTAAAACACACAAGCAAAACGTTTCCATGTAAACATC TATTTCTAAACTTATATAATGACCTGATTTTCTTTCTTGCAATTTTTCCCCCTTTTTAATTGAGAGGATC GTTCGAATGAGCCTCTGTGGCCTCTCCTTAGAGGCCTTCACAAGAAGCATAGACACTGTCCCTGACGGTA ATTAGAAGAAGCAGAGGCTCACACATCTCCTGGCACGGTCTTATCTCCCTCGTGTGTTGAACTTGCTGTC CGTCCATGCACCCTACGAAGGCCATGCTGTGCAAACGTGCTCAGGATCTCAGTAACGGGCCAACCCCCAA CTACAGATGACTTTTTAATATTGTAAAATATTTTCTGCTTTTTTGACTTGCATTTGAGAGTTTCTTGTTT CAGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30360903 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30360903 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12368 same embryo
	EMAGE:12370 same embryo
	EMAGE:12367 same embryo
	EMAGE:12366 same embryo
	EMAGE:12369 same embryo
	EurExpress:euxassay_007014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source