EMAGE mouse gene expression spatial database

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EMAGE:12372

Car10 carbonic anhydrase 10 ( MGI:1919855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007149. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007149_01	euxassay_007149_02	euxassay_007149_03	euxassay_007149_04

euxassay_007149_05	euxassay_007149_06	euxassay_007149_07	euxassay_007149_08	euxassay_007149_09

euxassay_007149_10	euxassay_007149_11	euxassay_007149_12	euxassay_007149_13	euxassay_007149_14

euxassay_007149_15	euxassay_007149_16	euxassay_007149_17	euxassay_007149_18	euxassay_007149_19

euxassay_007149_20	euxassay_007149_21	euxassay_007149_22	euxassay_007149_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12372_wholemount_strong.wlz

12372_wholemount_moderate.wlz

12372_wholemount_weak.wlz

12372_wholemount_possible.wlz

12372_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12372_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4311
Entity Detected:	Car10, carbonic anhydrase 10 ( MGI:1919855)
Sequence:	sense strand is shown >T4311 GTCGGGTGGGAGCGCGAGTGTGCGCTCTCTGGAGAGAGTGTGCGTGTGTGTATGTGTGTACCGTGTCGGG CTCCCCCCTTCCCCCCTGTTTTCCCGTCGAGTGATGCACCTGGAATGAGAATCAGAGGATGGAAATAGTC TGGGAGGTGCTTTTTCTTCTTCAAGCCAATTTCATCGTCTGCATATCAGCTCAACAGAATTCACCAAAAA TCCATGAAGGCTGGTGGGCATACAAGGAGGTGGTCCAGGGAAGCTTTGTTCCAGTTCCTTCTTTCTGGGG ATTGGTGAACTCAGCTTGGAATCTTTGCTCTGTGGGGAAGCGTCAGTCCCCTGTCAACATAGAAACCAGT CACATGATCTTCGACCCCTTTCTGACACCTCTTCGCATCAACACTGGGGGCAGGAAGGTCAGTGGGACCA TGTACAACACTGGAAGGCATGTCTCCCTCCGCCTGGACAAAGAGCACTTGGTCAATATTTCAGGAGGGCC CATGACATACAGTCACCGCCTGGAAGAAATCCGGCTACACTTTGGGAGTGAGGACAGCCAAGGGTCTGAG CACCTCCTCAATGGACAGGCCTTCTCCGGGGAGGTACAACTCATCCACTATAACCACGAGCTATACACAA ATGTCACAGAAGCTGCGAAGAGCCCGAATGGATTGGTGGTAGTTTCTATATTTATAAAAGTTTCTGATTC ATCAAACCCATTTCTTAATCGAATGCTCAACAGAGATACTATCACAAGAATAACATATAAAAATGATGCA TACTTACTACAGGGACTTAATATAGAGGAACTGTATCCAGAGACCTCTAGTTTCATCACTTATGATGGGT CGATGACTATCCCGCCCTGCTATGAGACAGCAAGTTGGATAATAATGAACAAGCCTGTGTACATAACCAG GATGCAGATGCATTCCCTACGCCTCCTCAGCCAGAATCAGCCATCGCAGATCTTTCTGAGCATGAGTGAC AACTTCAGGCCTGTCCAGCCACTCAATAATCGCTGTATTCGCACCAACATCAACTTCAGTTTACAGGGGA AGGACTGTCCTAACAACAGGGCCCAGAAGCTACAGTACAGAGTAAACGAATGGCTCCTCAAGTAGGGAAC TAAGCCAAGAAGAATCCCACCTCAGTGAAATTCTACAACTGTGAATTGACGCAACCCAGAATGCCCCCCT TCCCTGTCCTTCCTCCCCTAAGCCTTTCACTCTTTGGATTGGCCCCTTTTTCATGAAAATTGTCTGAAAA AATGTTTCAGAGGAGTGCATCTGTCTCTCTCTCTCTCTACACACACACACTCACACACACACTCATACAC ACATATACACATACACAGACACATATACACACACATAAATACAAATGCACACACTCATACACACATAAAC ACAAGTACACATGCACACACACACACACACACACACACACACACACACACACACCACCTCCATGATGAAA AAGCCAAGTTTCAGAAAAAAAGGTTCATTCATCAAAGGTCTTAGAAGAAAACAACCAGTTAACCTGATTA CAATTTTGATACTGTTTTCCTGAACTAATAACTCAACCCAATGAGACTTCTCAGCCTTTGTATATACAAA ATTCTTCCAAAAGAGAGTGGGGGGGGGGGGGAAACCACCGTTCTGACAGCATCAAGCGGACTCGGCATCT CACCATCCCTGACAGTGTCCTGGGTTACTCCGAGGGTGCTGTGACAGGTGACCAGGACAAAGCTGACCAA GGACACTTATTTCTAGATTACGATTCTTCTGTTTACTCAATGATTTAAAAAGAAAATCTTCAAAAATAAA TAAATAAATAAATAAATAAATAAATGGACAGACATCAAAGAGCTATACGTTGTATATATTTATCACCACA GACTATAAGAATGGAATTATTTCCCTCTTTGTCACATAACTGTACTAGGACTTGCCCGAGATCAGAATCG ATCCATTCGCTGTTTCTTGTTTTCCGAAGGTCATACATTGTGTTTGGTTATTGTTAACAGCCCAATAAAT GTGTTTAATGCGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4507552 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4507552 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12376 same embryo
	EMAGE:12374 same embryo
	EMAGE:12373 same embryo
	EMAGE:12375 same embryo
	EurExpress:euxassay_007149 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source