EMAGE mouse gene expression spatial database

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EMAGE:12374

Barhl2 BarH-like 2 (Drosophila) ( MGI:1859314)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007161. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007161_01	euxassay_007161_02	euxassay_007161_03	euxassay_007161_04

euxassay_007161_05	euxassay_007161_06	euxassay_007161_07	euxassay_007161_08	euxassay_007161_09

euxassay_007161_10	euxassay_007161_11	euxassay_007161_12	euxassay_007161_13	euxassay_007161_14

euxassay_007161_15	euxassay_007161_16	euxassay_007161_17	euxassay_007161_18	euxassay_007161_19

euxassay_007161_20	euxassay_007161_21	euxassay_007161_22	euxassay_007161_23	euxassay_007161_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12374_wholemount_strong.wlz

12374_wholemount_moderate.wlz

12374_wholemount_weak.wlz

12374_wholemount_possible.wlz

12374_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12374_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

corpus striatum

strong

single cell

strong expression: see section 01 02 03 04 05 20 21 22 23 24 moderate expression: see section 06 07

olfactory cortex mantle layer

strong

regional

strong expression: see section 11 12 13 15 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 18

midbrain mantle layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17 moderate expression: see section 07 08 09 10 weak expression: see section 18 19

dorsal grey horn

strong

regional

strong expression: see section 10 11 12 16 moderate expression: see section 20

spinal cord ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18 19

neural retina

strong

single cell

strong expression: see section 01 02 03 04 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8416
Entity Detected:	Barhl2, BarH-like 2 (Drosophila) ( MGI:1859314)
Sequence:	sense strand is shown >T8416 CGGCGGCGGCTGATCCGCGGAGTAGCGTGGACCTCATGTAGAAATGACAGCAATGGAAGGGGCCAGCGGG TCGAGTTTTGGAATAGACACTATTTTGTCCGGTGCCGGTTCCGGGAGCCCCGGCATGATGAACGGGGATT TCCGATCGCTCGGTGAAGCGAGGACAACGGATTTTAGGAGCCAGGCCACCCCGTCACCCTGTTCGGAGAT TGATACCGTGGGAACGGCGCCCTCTTCACCGATCTCGGTCACCCTGGAACCCCCGGAGCCGCATCTTGTG ACAGACGGACCCCAGCATCACCATCACCTCCACCACAGCCAACAGCCGCCGCCACCATCCGCGGTCCCCG CGCAAAGTTTGCAGCCTTCGCCCCAACAGCAACCGCCGCCGCAGCCGCAGTCAGCAGCCCAGCAGCTGGG CTCGGCCGCCGCGGCCCCCAGGACTTCCACCTCTTCCTTTTTAATTAAGGACATCTTGGGAGACAGCAAA CCCCTGGCGGCTTGTGCACCCTACAGCACCAGCGTTTCTTCTCCTCATCACACCCCGAAGCAGGAGAGCA ACGCGGCACACGAGAGCTTCAGGCCAAAGCTTGAGCAGGAGGACGGCAAAACCAAGCTGGACAAGAGAGA AGACCCCCAGAGCGACATCAAATGCCACGGAACAAAGGAGGAAGGAGACCGGGAGATTACAAGTAGCCGA GAGAGTCCCCCCGTGAGAGCCAAGAAACCTCGAAAGGCCAGGACAGCTTTCTCAGACCACCAGCTCAACC AGCTGGAGCGTAGCTTTGAGCGGCAGAAGTACCTAAGTGTCCAGGACCGAATGGACCTGGCGGCCGCACT CAACCTCACTGACACCCAAGTCAAGACCTGGTACCAGAACCGAAGAACCAAGTGGAAGCGGCAGACTGCA GTGGGCCTGGAGCTGCTAGCCGAGGCCGGGAACTACTCCGCGCTGCAGAGGATGTTTCCGTCGCCTTATT TCTATCACCCAAGCCTGCTGGGGAGCATGGACAGCACCACGGCGGCGGCGGCGGCCGCGGCCATGTACAG CAGCATGTACCGAACTCCTCCAGCACCTCATCCGCAGCTGCAGCGGCCGCTGGTACCCCGCGTGCTCATC CACGGCCTGGGGCCTGGGGGACAGCCGGCCCTCAACCCTTTGTCCAACCCCATCCCAGGCACCCCGCATC CCCGGTGAGGAAGGAGGGACAGGAGGGACAGTGCCACAGCCCTCCAGGTTCCCACCTCACCCCGGACGGC TGCCCCGCCTCTTCCCGCACCAAACCAACTGGAATAGAGGCAGAGGAGTAAAAGGGGGAGCTCACCGGTG GGCTGGGGTCCCCAAGGACCAGCCAGCTCTCAGATCTCCATCTGCCCACTCAGAGGCAGGGCTGCAAAGC TCCCCGAGTGTGACTGGAGAAAATACTGAAAAGTGCAACCAGTAAGTGAAAACACCCATCCACCCACACA TACACACACACACACACACACACACACACACACACACACACACACACACACACACACAAAACGAACCCTC TAGTTCTTTCTTTCTTTCTTTTTTTTTTCTTTTAAACAAAATGACTTTAGGGACATGCAGCAGGGAGGGA GGGGGGAGGGCAAAGAGGGGAGAGACTGGATGGAGAGGAGCCTTCGGGCAGAGAGAATTGTTACTGGACA GAAGCCTGAAGTTCACCCCCAGGCAGAAGGTGGTACCCTGGACATCCAGTCTGCCTCAGAGCAGAGGAGG GTGATGGAGAACTTTGCACTGATTTTTCATGAGCTTTTTTTTTTTCTTTTGGAAAAGTGGCATGCTACAT AATATGAAAAAAATGTACATTTGAAAGACTGAGCGATGAGTGATATATCATATTTATTATATGTTGTCCA AAAAAGAGTCACTTATATACGTCAGTAATAACATGACTTTTCTTTTCATGTCTTTTTGATTCAGTAAAAT AAATGCTTAGACAAAAGTATAGATTTTTTGTGATTGAAAATTACAAAAATAAAGTTTATCTTTTTTTAAC AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5688311 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5688311 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12376 same embryo
	EMAGE:12372 same embryo
	EMAGE:12373 same embryo
	EMAGE:12375 same embryo
	EurExpress:euxassay_007161 same experiment
	MGI:4823391 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source