EMAGE mouse gene expression spatial database

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EMAGE:12375

Tal1 T-cell acute lymphocytic leukemia 1 ( MGI:98480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007171. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007171_01	euxassay_007171_02	euxassay_007171_03	euxassay_007171_04

euxassay_007171_05	euxassay_007171_06	euxassay_007171_07	euxassay_007171_08	euxassay_007171_09

euxassay_007171_10	euxassay_007171_11	euxassay_007171_12	euxassay_007171_13	euxassay_007171_14

euxassay_007171_15	euxassay_007171_16	euxassay_007171_17	euxassay_007171_18	euxassay_007171_19

euxassay_007171_20	euxassay_007171_21	euxassay_007171_22	euxassay_007171_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12375_wholemount_strong.wlz

12375_wholemount_moderate.wlz

12375_wholemount_weak.wlz

12375_wholemount_possible.wlz

12375_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12375_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 weak expression: see section 09 10

pons mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 weak expression: see section 06 07 08

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20

ventral grey horn

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

liver right lobe

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9150
Entity Detected:	Tal1, T-cell acute lymphocytic leukemia 1 ( MGI:98480)
Sequence:	sense strand is shown >T9150 GCTGAACGAGCATCTGGGAGGCGGAGATCCGAGTCGGGTTGTTTACCGCGCAGGACCTCACGGCAAGCTA AGTAACTGCTGGTCTCTCAGCGAGAGCCGGGAAACCCAGCTTCGGGGTCCTACCTCGACCCCTCTCCAGC GGAGGAGTGGAGGTCCTAACCAGCCGAGTAGGTCTCTCTAAATATGCCCCAGGATGACGGAGCGGCCGCC GAGCGAGGCGGCACGCAGTGACCCGCAACTAGAGGGACAGGACGCGGCCGAGGCCCGCATGGCCCCCCCG CACCTAGTCCTGCTCAACGGCGTCGCCAAGGAGACGAGCCGCGCAGCCCCGGCTGAGCCCCCCGTCATCG AGCTAGGAGCGCGCAGCGGCGCGGGGGGCGGCCCTGCCAGTGGGGGCGGTGCCGCGAGGGACTTAAAGGG CCGCGACGCAGTAGCAGCCGAAGCTCGCCTTCGGGTGCCCACCACCGAGCTGTGCAGACCTCCCGGACCC GCCCCGGCGCCCGCGCCCGCCTCGGCTCCTGCAGAGCTGCCTGGAGACGGCCGCATGGTGCAGCTGAGCC CGCCCGCGCTGGCAGCCCCTGCCGGCCCCGGCCGAGCGCTGCTCTATAGCCTTAGCCAGCCGCTCGCCTC ACTAGGCAGTGGGTTCTTTGGGGAACCGGATGCCTTCCCCATGTTCACCAACAACAACCGGGTGAAGAGG AGGCCCTCCCCATATGAGATGGAGATTTCTGATGGTCCTCACACCAAAGTAGTGCGGCGCATCTTCACCA ACAGCCGGGAACGATGGCGGCAGCAGAATGTGAATGGGGCATTTGCTGAGCTCAGAAAGCTGATCCCCAC CCACCCACCAGACAAGAAACTAAGCAAGAATGAGATCCTCCGCCTTGCCATGAAGTACATCAATTTCCTG GCCAAGTTACTCAATGACCAGGAGGAGGAAGGCACCCAGCGTGCCAAGCCTGGCAAGGACCCCGTGGTGG GAGCTGGTGGGGGTGGGGCAGGGGGTGGCATCCCCCCTGAAGACCTTCTACAGGACGTGCTTTCCCCCAA CTCCAGCTGTGGCAGCTCTCTGGATGGGGCAGCCAGCCCGGACAGTTACACAGAGGAGCCAACACCCAAG CACACTTCCCGCAGCCTCCATCCTGCCCTGCTGCCTGCCGCTGATGGGGCTGGCCCCCGGTGATGCGTCT GGGGCTGCCCAGGGCCAGCAGGCAGGGGCCTTTAGGCCCCTGGGTTGCTGGGCTTCAGGGCAGGTGGGAT GAGAAGCAGGTCAATGGACTTATGTGAACTTCCCTTACAGTTTGAACTTTGGGAAGTCCCAACTGACCCT AGGCTGGCATTTCTGTTTCCTGCATGGAAACAGAAGAGGCAAACAGAGTGAAGTAGTAGGTACTTTTTCT GAAGATGGCACGGTCTTCTCCCTTTCCCAAGCCCAAAGATTTCCCCAATGATGAGGCTCAAGTGTCTAGT TTTGGTCTAGAGTTTGTGAGCCTTGTTTTGGACAAGATCTGGGGTTGTCACCTGTTACCTGAGGCATCCA ATTGGCCTCTGCCTTGCCTTTCTCCCATCCCGAGCTGGCTGGGTGGCTTTTGTGGCAGCTTCTGTTCAAA TACTTAGGTACCCAAGAGCTGACCATTTCTTGAGCCCCATCTTCACCCAGGTCCATGTTGATCCATCCAG CTTGCCAGCTGCTGTGGAGAGTCAAGCTTTGAGGTGCCTTCTTCAGGGCCTGGTTGGAAAAGATGGCCAG TAAACAGACAACCTGCTCTCAACTAGCTGGGGCAGAAAACCCAACAGCCCCTTTTGCTCCAGGGAATCAG AGCTCTCTCCCTCACTGAGACTTGCCTTCCTATCCTGTGGCTGTGAGGACCAAGTCAGCAGCCTTGAGCA TGCTTTGTTGGGCTGTGTGCAGGCACAGAAAGGGAAAGTGACATTAGGGCAAGCAAAGCAAGAGTGTTTG GGGCACTGGGTTTCAACTCCAGCGCATCCCTGTTTGTGCAGGAGAGCAAAGGATAGAGTGCTGGTGCATT GCATGTTGGTGCTGGGGCTGTGCAGCCCATAGGCTTCCCCAGGAAGCCCGAAGGAAGCTTCCCATTGCAA GATGTCTGTTGGCTGATGGTCTGGATGTCCAAATGGAGGACAAACTGTCCTGTACATACTCTATTTGGGA TTCTGCCTGACCAGTTGTTGGTTGGCAACTCTAGGCCATGATACCACCCAAGATGCAGCCTGATGCTAAG GCAAGGAGTCCAGTCAGAGGCAAGGCCCTGGCTGAGACCTGGGCATGCACCTTTGCTCAGCTTAACTTTA AGTACTCTCCCCTCACACAACTGCAGGCCCTACGTGTGTGCATGGTAGGTTGGCTTAACATGAAACGAGT GCCCCCTCTAAAACTTGTACATGAAAGTTTTGTAAAGCAGACCCTTCTCCTCCATCTTTTAAAGACACAA CCCCTGCAAGTCATTTTGTAAAGTGGAGAATCCTTTTGTAGTATGGACAAATACCCCATTGTTTCTCCCC TGTGTCAATCCAGGTGGTGGGACCGATCTATCCTAAAGCTAGGCCTGCCTATAATGAAGTTCAAGCTCAT GAAACAAAGTGCGCAGAAGTCCTGTGTATCTGTCATTGTATTGGTGTCACTCAGTTCTCACTCTCTCCAG GGTTTGCCTTTGAGCTAGAGAGAAAATATCCAAAGGCCCCATCATGTAACGTATGAGCCCATCACTTTTC TGTTGGAGTTTCAGCATGATGTTTCAGTTCACACCATGATGGAGCCTGAGTATAGACTTAGGCAGACAGA GACTGATCCTGTTCCTGCTCCCCTCCCCTTCTTATTAAGTACTGAGGGCCCCCAGGTAATGGGACAATGT GGTCAGCTACTAAGGCATTGTGATTCTTTGTCTCCTATCACATTCTGCTATCACATGAACATTAAGGCTG GTTCTTTCCCATTGCGTGGGAGTTAGTTCAAGGGGTCTGACCCATGAGGAAATGGGTCCAAAGCTGTGTT TGCTTCTTAGTGGAATGAGTTGGGAATGAAAACTGTGGCTCTGTAGGTGGATTCTATGAGAGGGATCACA CTTTTGTTGGGAGCCTAAGCTGCAGTTCCCTACATCAGCTTTGCAGCTTCACTGGGATAAACACGCTAGG CGGTGGACGGACTGTGATCATAACATTGGAAGTCCATTGGGTCCGTAAAGACTGCTCTGGAGCACAGAGG GCCGGTGCATCGCGGGGGGGGGGGGGGCGGGGATTGCACACACGGGGTTCTGTGTTCTACACCCAGCACA GGCACAAAAGAAGCTTCATAACCCTTAGTACTGACCGTGCCGTGAGGTTCCAGCCAGAGTTCAGGCCTGG GCAGTTGATGTGTTTGTGTCATTTGGATTATAAAATATTTTCCATTGAGTTATGTAGATGCTAATTTCTC AAGATTAATCTTAATTTCTCCCCGATTCCAAGGCAAGAAACAGGAGTGAGAAGGGGTGAGAGATTTCGAA AGTTTCTAAAGGGGCTGGGCAGGGAAGAGCCAGGGTTTTGGTCTTTGTAAGATCTAAGGAAGACCCTGAA TTCTGTTCTCATACTCCATACCCCATATCTTTCTTCCTCTGTGTCTTCCTTGCCCTTAAAGAAATTGCAG CATTCCAAGAACAATATCTGTACAAAGGGGGAAATGTAAGCATGAGAAAACATTAAAAAAAAAAAACAGT GATGAACATAACCACAGAGAGAATCCCACCCTTCAAGAATAATTCATGTTTATTTGTGGTGGCAAATAAC AAAATGGTACAACCTTTATCCTTTTCCAGAAACAAAAACCAAGGGCACAGCAACTAGAGTGAGCTGACAG CTATTTTGGCCTTTTTGGTGGGTCTAGCCGTACTTGGGATCCCAGTGGTACATGACCCTCTGCCGAAGGC TTGCCTCAGTCTGTGTACATAGCACGCCATGTCTGTGGGCAAGCCCAGCACTTTGCGTCAGTGTCGTACT GTATGTAATGAACTGTGTTGGTCTCTGTGTTTTTTTTTTCTGAAGAAGAGGAGTAACTACTCCGGGTACC TTGATATTTGTACAGCCTATAGGCCAACACTGCGGGCGTGTGACTCTTTATTGAAAAACAAAAACAAAAA AATACCAGTGTGGTGATGATAGTGTGTGTATATATATATAAGGTTATATGGGGAAGATTTCTAAATAAAA GTTTTACAAAGGGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6826611 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12376 same embryo
	EMAGE:12372 same embryo
	EMAGE:12374 same embryo
	EMAGE:12373 same embryo
	EurExpress:euxassay_007171 same experiment
	MGI:4828593 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source