EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12388

Dmd dystrophin, muscular dystrophy ( MGI:94909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010997. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010997_01	euxassay_010997_02	euxassay_010997_03	euxassay_010997_04

euxassay_010997_05	euxassay_010997_06	euxassay_010997_07	euxassay_010997_08	euxassay_010997_09

euxassay_010997_10	euxassay_010997_11	euxassay_010997_12	euxassay_010997_13	euxassay_010997_14

euxassay_010997_15	euxassay_010997_16	euxassay_010997_17	euxassay_010997_18	euxassay_010997_19

euxassay_010997_20	euxassay_010997_21	euxassay_010997_22	euxassay_010997_23	euxassay_010997_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12388_wholemount_strong.wlz

12388_wholemount_moderate.wlz

12388_wholemount_weak.wlz

12388_wholemount_possible.wlz

12388_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12388_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 07 21 22 weak expression: see section 08 09 19 20

cerebral cortex ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 12

pons mantle layer

weak

regional

weak expression: see section 16

pons ventricular layer

weak

regional

weak expression: see section 12

spinal cord lateral wall

weak

regional

weak expression: see section 13 14 15

lower jaw incisor

weak

regional

weak expression: see section 11 12 15 16

lower jaw molar

weak

regional

weak expression: see section 19

upper jaw incisor

weak

regional

weak expression: see section 11 12 16 17

upper jaw molar

weak

regional

weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36288
Entity Detected:	Dmd, dystrophin, muscular dystrophy ( MGI:94909)
Sequence:	sense strand is shown >T36288 CAAGAGTGAATTTTCCCTGGAGCCCAAAGCCAGGAGACTACCGCCACACTAAAATATTGTCTAGGGCTCC AGATGTTTCTAGTTTTAAACTTTCCACTGAGAGCTAGAGGATTCATTTTTTTCAAGGAACATGCGAATGA ATACACAGGACTTACTATCATAGTAATTTGTTGGCTGATATATTCAACTTCCTACTGTTGGGTTATATTT AATGATGTTTCTGCAATAGAACATCAGATGACATTTTTAACTCCCAGACAGTAGGAGGAAGATGGTAGGA GCTAAAGGTTGCGGCTCCTCAGTCAATTTATATGAGGGGAGCAACAACTCTGTAAAAGAATGGATGAATA TTTACAACTATACATATAAACATCTCTATAATTACAACTAAATTGTTCTGCCCTCTTCATAAACTCAACC TGAAGTGGGTGGTTTTGTTGTTGTTGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97664. Forward Primer - name:097664_F_cDNA_Dmd, sequence:CAAGAGTGAATTTTCCCTGGAG; Reverse Primer - name:097664_N_SP6_cDNA_Dmd, sequence:ACAACAACAACAAAACCACCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12390 same embryo
	EMAGE:12387 same embryo
	EMAGE:12389 same embryo
	EurExpress:euxassay_010997 same experiment
	MGI:4824331 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source