EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12390

Ip6k1 inositol hexaphosphate kinase 1 ( MGI:1351633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008353. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008353_01	euxassay_008353_02	euxassay_008353_03	euxassay_008353_04

euxassay_008353_05	euxassay_008353_06	euxassay_008353_07	euxassay_008353_08	euxassay_008353_09

euxassay_008353_10	euxassay_008353_11	euxassay_008353_12	euxassay_008353_13	euxassay_008353_14

euxassay_008353_15	euxassay_008353_16	euxassay_008353_17	euxassay_008353_18	euxassay_008353_19

euxassay_008353_20	euxassay_008353_21	euxassay_008353_22	euxassay_008353_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12390_wholemount_strong.wlz

12390_wholemount_moderate.wlz

12390_wholemount_weak.wlz

12390_wholemount_possible.wlz

12390_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12390_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9159
Entity Detected:	Ip6k1, inositol hexaphosphate kinase 1 ( MGI:1351633)
Sequence:	sense strand is shown >T9159 GTTGATCCGTACCCAGTGGGCAGCGCCAGGAGCTGGACCGAGCGGCCGGTGAGGGGCCGCTGCTGCGGGG CTCAGCCACCAGCGCCGCCTGCCAGGGGGGCGGGCCGAGACCTGGAGGCCCGGGGGGCCCAGCTCCCGTG GGGAGCCTTGGGCGCCCGCTGCCCGAGTCGGGCCGGACAGAACAGTAAACTAAGCAGACTGTGACCATTG GCCTCCCACTAGCCAGGATCAACTTAGCTCTCCTTTTGTGGTCCATGTGCTGCATCGTCTGCCATCAGTG TGCAACCGGCCCCCAACGCAATGTGTGTTTGTCAAACCATGGAAGTGGGGCAGTATGGCAAGAACGCAAG TCGGGCCGGAGACCGAGGAGTCCTCCTGGAGCCCTTCATCCATCAGGTGGGCGGGCACAGCAGCATGATG CGGTACGACGACCACACTGTGTGCAAACCTCTCATCTCCCGGGAACAGCGCTTCTACGAGTCACTCCCTC CCGAAATGAAGGAATTCACCCCAGAGTACAAAGGCGTGGTTTCTGTCTGTTTTGAGGGAGACAGTGATGG TTACATCAACTTGGTAGCCTATCCTTATGTGGAGAGTGAAACTGTAGAGCAAGATGACACACCAGAGCGG GAGCAACCTCGGCGCAAACACTCCCGCCGGAGCCTGCATCGGTCTGGCAGTGGTAGTGACCACAAGGAGG AGAAAGCCAGCCTGTCCTTTGAGACCTCTGAGAGCTCCCAGGAGGCAAAGAGTCCAAAGGTGGAACTGCA CAGCCACTCAGATGTCCCTTTCCAGATGCTAGACAGCAATAGCGGTCTGAGCTCCGAGAAGATCAGCTAC AACCCCTGGAGCCTGCGCTGCCACAAGCAGCAGCTGAGCCGCATGCGCTCCGAGTCCAAGGACCGAAAAC TCTACAAGTTCCTCCTGCTTGAGAACGTGGTACACCACTTCAAGTACCCCTGTGTGCTGGACTTGAAGAT GGGTACCCGGCAGCATGGCGATGATGCTTCAGCTGAAAAGGCTGCCCGGCAGATGAGGAAGTGTGAGCAG AGCACGTCGGCCACGCTGGGGGTCAGGGTCTGTGGCATGCAGGTGTACCAGCTGGACACAGGTCATTACC TCTGCAGGAATAAGTACTATGGCCGTGGACTCTCCATCGAGGGCTTCCGCAATGCGCTCTATCAGTACCT GCACAATGGCTTGGACCTTCGACGTGACCTTTTTGAGCCAATCCTGAGCAAACTGCGGGGCCTCAAAGCC GTACTGGAGCGGCAGGCCTCCTACCGCTTCTACTCCAGCTCCCTGCTTGTCATCTATGATGGCAAGGAGT GCCGGTCTGAGCTACGTCTCAAGCACGTGGACATGGGGCTCCCTGAGGTGCCACCACCCTGTGGCCCCAG CACCAGTCCTAGCAGCACCAGTCTTGAGGCTGGCCCCTCTTCTCCGCCCAAGGTGGATGTTCGCATGATT GACTTTGCACACAGCACATTCAAGGGCTTCCGGGATGACCCCACTGTTCACGATGGGCCAGACAGAGGCT ATGTGTTTGGCTTGGAGAATCTTATCAGCATCATGGAGCAGATGCGGGATGAGAACCAGTAGGCCCTGTG CTGGGCCTAGGACCCCCCTCCTCTCCACCCACAGGCAGGGACCATTGCTCTGAACTTGCTGTGAGGACAC ACAGACTTGCTTTTAAAGGGTTATATTTCTCTTTGGTGTAAACTAAAAGAAATGTTTTTAGCTGTAGCCT GGAATCCATATATATAAAGTAGAGGAGGGCAGAACATTTGTCCTCTCAGCCAGGCTCCTTAGCTCTGTGG CTGTGACTGCTGTGTCCAGGCTGTCTCAGGAAGGAAGAGGTGGCCCCAGTGGGCTTGACAGCAAAGAGAA TGAATGCCTTGAACTTTGGTCTCCCTTGCCTAAGGCGTTTGGGATCTGTGGCTGTGGGGCTCTGCTGATG GTGAGGTGAAGCCCTCGGCTGGCACACGGTCTCCATGCCCAATCATCCCTTATTTCCCAGAACTGGGGAA GGTTAAGTGCCCACGTCCTGAGGGCCATCTTAGTTTCGTGCCAGTGCTGTAACAGATGCCTCAGGGCATT AGAGGGCTAGCTGCACAGACGGACTCACCCTTGGGTCTGATTTTGGCATGGGGCTAGATCGGTGAAACTG AGCTAAGGCCAACTCAGCAGAGGGCAGGCCCTGGGCTCCCTAGGCACCCACTGGCATGTAACCCTGTTCC TCTCTCCACAACTCGAGCCAGCCTCTTGCTGGGATCCCTGACCTAAGGAGGCCTCATAGCCTCCTATCCC ACTAAGATGGGCACAGCTGTTTGCTGAGGAGAGCCAGTGTCCCAGGAGGCCTAAGGGCGGGACTTTAGTG GCTCTGGCCCAGACAGTATTTGCAGTTCTGGGCTCTGGGTGGCATCCCTTCCTCACCTGGGGCAGCTGTG CTGCTTGTGGGTGGGCTGCTGCTCCCAGGGCTCAGGAACCGTGGTCCGCTCAGGGTCTTGTCTTCTCCAG GCCAAGCTCAAGGCAGCAGCCCTGTATGTGGTGCTCCAGGCTCTAGGCCTGGAGGGAGAACTTTAAACTT GTTTGCTTTTAGGGACATAGGGCCCTGGGCACAGGTGCCCAGCGTCTGCTGAGAGCTTTGATTAATCCTG GGGTTTGTCAGTAAGTCCATTTGTCCACCTGCTAGCCCCTGTCTCATGGTGAGCTTTCTGGAGTATAGCC TTGTTGGTTAGTATATATTGATTTCCTTCATGCTTCCCCCAGCAGTCTTCTCCTTTCCTTTTCTGAGGTG AGTCTGCTCCTGTGCCCCTCCTCAGCAGGTTCTGCTGTTACAAACCTGGTGGCCAAATGATTCATAGTGG TGTTTACCTGCTAGCCCAGTGGCCTTCTTGTGGGCCTGGGGTATGGCCTTCAGCACCAACGGTGGGTAAT GAAGGCTCAGGGTTGACCCTGACCTATTGCTTTTATTTCTGCTACCAGTCCTTTCATGGTAGAGACCTGC TGCTGCCTGTAACATAGTGTCCGACAGGTTACACTACACCAATCATTGCCAAGGTGAGCAACAGTCTCCA AGTCTATCACTTCTAACATGTCTCTGTTTTGGCATTTGAAGGTAATCCTGCCAACCTTTGAGAGAAACTG TTAACGAATGGCATAATTTCCAGGTTCTTAGAGATGCAGCCTCTGAAAAGGGTTCTGTGACGGATCCAAG TCAGTCTTCTGACCCTGGTGCCATTGGTGGGCAGGTTCCCATTCCTTAGGGCCGGGAGGCACGGCTTGGC CCATCTCTGCTTACTAACACCTGTCTTCTGTCCAGTAGTAGTGTTGCTTGTGCCATGCAGGCAGCAAGTG TTAATTAGCGTGGACACCTTTTCTTTGGCAGGATAGAGGTCAAGAGAGGAGTTTGGGGCCAGCAGAGACC TCCTAGGAATGTGCTGTACTTCCGATTTACCAGATTTTGGGAGGTGCTGCCCCCATTCTGCTGCTTCTCA AGTAGCAAGGGAGTTGGTAGGCACTGCTCCTGCCACTGCAAGCGAGGAAAGGCTGGGATTTGCCGCTGCT CAAGGGCAGGGCCTCACTTTTCAGCACTTTTGATATCTGGAGGCCCAGCTCTGTTATCTGATAGGTGGTG GCAAGTGTGTGCGAACACTTTTACCACTGCTCTTCTGCCTGTCCCAGGCTAGAGCCCTGTCTTTTTCCTG ACAGGTCCTGCCTTCCCAATCATCAATTTGGGACCAAAGTGCAACTAGGGATCATGGGTTGGGAGCTCAC ATTACCCCTTTCCATTGTATTTCCCGGGCCAAGCCCATACCAGCCCGGGGAGATGGGTGGGAGTGAAATG GGATGGGTGCTGCTGGAAGAGGAAGGGACCAGTAGAGCAATGTCCTTGCCTGGGATTCTACCCCTCTCAG CTGTGGGCCGTGCAGTGGTGTGGGGATTCATTCTCTCCTAGGGGCAAGGGACCTGGGCTCGGACAGTATC CGTTCAGTATCCGCTGCTCATTCGCTCACATCTGGCCACGTGCACGTTCCCTAGATGCAAACTGCTTTGA ACTTTACAGCTATATAAGTTGGTTGTGTGTGTGTGCTCATTTTAAAAACTACATTTTAACGAGGAAAAAG GAGAACCTGGGGAAATTTCCTTTGCTTCTGTGGAGGAAATGTAAGACTTGGAATTCTGGAAATTGTTATC TGCTCCTCTTTCCTGGAAGCTACACACCCTAAGACCTGCGCCTGGATGCATACGTGGCTCCACTGGCGCT TAGGCGCGCTTGCATTTTCCCCAGGTCTTCTGTGTGGTGGAGAGGAGTGCTTGGCCCAAGCCGGCCCTTT TCTGTACACCCAGCGCTATCCGCCCGCTTGTGTCTGGTCGTATACGTCAAAATAAAGCCTCTAGAAACTG AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827203 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827203 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12387 same embryo
	EMAGE:12388 same embryo
	EMAGE:12389 same embryo
	EurExpress:euxassay_008353 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source