EMAGE mouse gene expression spatial database

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EMAGE:12399

Map3k6 mitogen-activated protein kinase kinase kinase 6 ( MGI:1855691)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011224. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011224_01	euxassay_011224_02	euxassay_011224_03	euxassay_011224_04

euxassay_011224_05	euxassay_011224_06	euxassay_011224_07	euxassay_011224_08	euxassay_011224_09

euxassay_011224_10	euxassay_011224_11	euxassay_011224_12	euxassay_011224_13	euxassay_011224_14

euxassay_011224_15	euxassay_011224_16	euxassay_011224_17	euxassay_011224_18	euxassay_011224_19

euxassay_011224_20	euxassay_011224_21	euxassay_011224_22	euxassay_011224_23	euxassay_011224_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12399_wholemount_strong.wlz

12399_wholemount_moderate.wlz

12399_wholemount_weak.wlz

12399_wholemount_possible.wlz

12399_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12399_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 21 weak expression: see section 03 04 05 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36617
Entity Detected:	Map3k6, mitogen-activated protein kinase kinase kinase 6 ( MGI:1855691)
Sequence:	sense strand is shown >T36617 AGCAGAGAATCTCCTGGAACAGGAACAGGACTCTCGACTCAGCAAGATTCATGTGGAACTTCTGCTTCGT GGCCTTGGGGCACAAATCCACACTCCTAACCGCCGGCAGCTGGCCCAGGAGCTGCGGACCCTGCAAGCTC AGCTGCGGGCCCAGAGCCTGGGCCCTGCGCTTTTGAAGGGGCCGCTCTTCGCCTTTCCAGACGCGGTGAA GCAGATCCTCCGCAGACGCCAGATCCGCCCACACTGGATGTTCGTGTTGGACTCGCTGCTCAGCCGTGCA GTCCGGGCGGCGCTGGCGGTGCTGGACGCGGAGTCGGAGAAGAAAGCGGTCTTACCGAGGTCAGAAGAGT CGAGTAAAGAGGAGTCCCAGCAGAAACCTCAGGAGAGCCAGGCGCTGCAGAGCCAGCTCCCACCAGAGCA GGGACCCCCATCGTTGATGGTGGAGTTGGGCCTTTTGCGAGCCGAGACTGACAGGCTTCGGGACCTTCTG GCTGAGAAGGAACGTGAGTGCCAGGCGCTGGTGCAACAGGCCCTGCATCGGGTGCATGCAGAGACCAGGA AGTATGCCCCAGCTTCCGAGACCCCAGCCACTCTCCCAAAGGACCAGAACCTGGTGCGGTGGCTACAGGA ACTGAGTGTAGATCCAGCCACTATCCAAACGCTCCTGAGTCATAGCTTCACCCTTCAAACCCTGCTCACC TGTGCCACTCAAGATGATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100034. Forward Primer - name:100034_F_cDNA_Map3k6, sequence:AGCAGAGAATCTCCTGGAACAG; Reverse Primer - name:100034_N_SP6_cDNA_Map3k6, sequence:GATCATCTTGAGTGGCACAGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12403 same embryo
	EMAGE:12402 same embryo
	EMAGE:12400 same embryo
	EMAGE:12401 same embryo
	EurExpress:euxassay_011224 same experiment
	MGI:4826083 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source