EMAGE mouse gene expression spatial database

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EMAGE:12400

Ctps2 cytidine 5'-triphosphate synthase 2 ( MGI:1933185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011214. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011214_01	euxassay_011214_02	euxassay_011214_03	euxassay_011214_04

euxassay_011214_05	euxassay_011214_06	euxassay_011214_07	euxassay_011214_08	euxassay_011214_09

euxassay_011214_10	euxassay_011214_11	euxassay_011214_12	euxassay_011214_13	euxassay_011214_14

euxassay_011214_15	euxassay_011214_16	euxassay_011214_17	euxassay_011214_18	euxassay_011214_19

euxassay_011214_20	euxassay_011214_21	euxassay_011214_22	euxassay_011214_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12400_wholemount_strong.wlz

12400_wholemount_moderate.wlz

12400_wholemount_weak.wlz

12400_wholemount_possible.wlz

12400_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12400_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6248
Entity Detected:	Ctps2, cytidine 5'-triphosphate synthase 2 ( MGI:1933185)
Sequence:	sense strand is shown >T6248 GGGCCGGCTGCCGGACTGCGAGGGGAAGATTGCATCTGGGTATTGGCAGTCACCTATTCGCCCGCCAATG AAGTATATCCTGGTTACTGGTGGGGTCATCTCAGGCATTGGTAAAGGGATTATCGCCAGCAGCATTGGGA CTATTCTCAAGTCATGTGGACTCCGAGTTACTGCCATCAAAATTGACCCCTATATTAACATCGATGCTGG AACTTTCTCACCTTATGAACATGGTGAAGTATTTGTTTTAAATGATGGTGGAGAAGTTGATTTGGACCTT GGAAACTATGAAAGATTCTTGGACATTAATCTCTATAAAGACAACAACATCACCACTGGGAAGATATATC AGCATGTGATCAACAAAGAGAGGCGCGGTGATTACCTGGGGAAAACTGTTCAAGTTGTCCCCCATATTAC TGATGCTATCCAGGAGTGGGTTATGAATCAAGCCAAGGTGTCTGTGGATGGCAATAAGGAAGATCCCCAG ATATGTGTTATTGAGCTGGGAGGCACCATTGGAGACATTGAAGGAATGGCATTTGTGGAAGCATTCCGAC AGTTCCAGTTTAAAGCCAAGAAAGAGAATTTCTATAATATTCATGTCAGCCTTGTGCCACAGCCCAGTGC TACTGGAGAACAAAAAACCAAGCCCACGCAAAACAGTGTCCGTGCGCTGAGAGGGTTGGGCTTGTCTCCG GATCTGATCGTCTGCCGTAGTTCAACCCCCATTGAGATGGCTGTGAAGGAGAAGATTTCTATGTTTTGTC ACGTGAACCCTGAACAGGTCATCTGTATCCATGATGTTTCTTCCATCTACCGAGTGCCTCTCCTTTTGGA GGAGCAAGGTGTGGTTAAATATTTTCAAGAGAGATTGGGCCTGCCCATCAATGATTGTTCAAGTAATTTG CTTTTCAAGTGGAAAGCCATGGCTGACAGGTATGAGAGATTGCAGAAAATATGTTCCATAGCCCTGGTTG GTAAATACACCAAACTCAGGGACTGCTACGCCTCTGTGTTCAAAGCACTGGAACACTCAGCCTTGGCCAT CAACCACAAGTTGAACCTGATGTACATAGACTCCATTGATCTGGAGCCAGTCACCAAAGCCGAGGATCCT GTGAAATTCCATGAAGCTTGGCAGAAGCTGTGCTTAGCTGATGGGATTCTTGTGCCCGGAGGGTTTGGAA TCCGAGGAACATTGGGAAAACTCCAGGCAATTTCGTGGGCAAGGACAAAGAAGATTCCATTTCTGGGAAT TTGCCTTGGGATGCAACTGGCAGTGATTGAGTTTGCAAGAAACTGCCTGAACTTGAAAGATGCTAATTCT ACAGAATTTGAGCCAAACACTCCAGTCCCATTGGTAATTGACATGCCTGAACACAACCCTGGCGATTTGG GAGGAACAATGAGACTGGGGTTAAGACGAACTGTTTTCACAACTGAAAACTCCATACTGAAGAAGCTTTA TGGTGACGTTCCTTACATAGAAGAAAGACACCGACATCGCTATGAGGTGAACCCTAACCTGATCAACCAA TTTGAGAATAAAGACCTTTGTTTTGTCGGTGAGGATGTAGATGGGAAGAGGATGGAAATTGTTGAGTTGA CAAGTCATCCATATTTCATTGGTGTCCAGTTCCATCCTGAGTTTTCCTCTAGGCCAATGAAGCCTTCCCC TCCGTACCTGGGGCTCTTACTTGCAGCAACGGGGAATCTGAATGCCCATCTGCAGCAGATGAACAAGCTG CCATACAGTGATGGATACAGTGATGCCAGCGATGACAGCTTTCCAGAGGCAAAGCTTGCTGAACTGGATC TCAACTGAAGCTCTTTCTTGCCTCAGGAATAGCAGAAGCTACCTAAGAGGCCTCTGAAGTCATTATACGG AAGATGAAGGAATTGTCTGAAGAAACCACCACACTGTTCCCTAGACATGGGCTGTGAAGATTCAAAGAGA ATCTGACAATGTTTACTTCATGCAACAGAACCAAAGGGGTCCTTTTCTTTTCCCTCCAGAGGCAGCCTGT AGTAGTCACACATTTCTATCCTTAATTAAACGCAATTCAGCAGCAAGCCCGGGCCCGGCAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3500778 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3500778 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12403 same embryo
	EMAGE:12402 same embryo
	EMAGE:12401 same embryo
	EMAGE:12399 same embryo
	EurExpress:euxassay_011214 same experiment
	MGI:4824109 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source