EMAGE mouse gene expression spatial database

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EMAGE:12439

Cand2 cullin-associated and neddylation-dissociated 2 (putative) ( MGI:1914338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011212. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011212_01	euxassay_011212_02	euxassay_011212_03	euxassay_011212_04

euxassay_011212_05	euxassay_011212_06	euxassay_011212_07	euxassay_011212_08	euxassay_011212_09

euxassay_011212_10	euxassay_011212_11	euxassay_011212_12	euxassay_011212_13	euxassay_011212_14

euxassay_011212_15	euxassay_011212_16	euxassay_011212_17	euxassay_011212_18	euxassay_011212_19

euxassay_011212_20	euxassay_011212_21	euxassay_011212_22	euxassay_011212_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12439_wholemount_strong.wlz

12439_wholemount_moderate.wlz

12439_wholemount_weak.wlz

12439_wholemount_possible.wlz

12439_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12439_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23 weak expression: see section 10 11 12 13 14

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 15 16 moderate expression: see section 12

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22 23 moderate expression: see section 12 14

rest of cerebellum marginal layer

strong

regional

strong expression: see section 02 03 04 08 17 18 19 20 21 moderate expression: see section 05 06 07 12 15 16 weak expression: see section 09 13 14

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 03 04 08 17 18 19 20 21 moderate expression: see section 05 06 07 12 15 16 weak expression: see section 09 13 14

pons ventricular layer

strong

regional

strong expression: see section 08 moderate expression: see section 06 07 12 15 16 weak expression: see section 13 14

midbrain ventricular layer

strong

regional

strong expression: see section 08 moderate expression: see section 09 10 11 12 13 14 15 16

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 15 16 weak expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9125
Entity Detected:	Cand2, cullin-associated and neddylation-dissociated 2 (putative) ( MGI:1914338)
Sequence:	sense strand is shown >T9125 ATTCGATTCGACCCGCAACCCCGCGGCGCAGCCAGCATGAGTACCGGTGCCTTCTACATCTCCAGCCTGC TGGAGAAGATGACGTCCAGCGACAAAGACTTCAGGTTCATGGCTACCAGTGACCTGATGTCAGAGTTGCA GAAAGACTCCATCCAGCTGGATGAGGACAGCGAGCGTAAGGTGGTGAGGACGCTCCTGCGGCTCCTGGAG GACAGGAGTGGTGAGGTGCAGAACCTGGCTGTCAAGTGCCTGGGTCCCCTGGTGGGCAAGGTGAAGGAGT ACCAAGTGGAGAACATTGTGGACACCCTCTGTGCCAACATGCGGTCAGACAAGGAGCAGCTCCGGGACAT CGCTGGCATTGGCCTGAAGACCGTGCTTTCAGAGCTGCCTCCCGCCGCCACAGGCTCAGGGCTGGCTATC AATGTGTGTCGAAAGATCACAGGCCAACTCACCAGTGCCATCGCCCAGCAGGAGGATGTGGCTGTGCAGC TGGAAGCCCTGGACATCCTCTCTGACATGCTGAGCAGGCTAGGTGCCCCGTTGGGCACCTTCCATGCAAG CCTGCTGCACTGCCTATTGCCCCAGCTGAGCAGCCCACGCCTGGCTGTGCGCAAGCGCACTGTTGTCGCA CTTGGTCATCTGGCAGCCGCCTGCAGCACTGACCTCTTCGTGGAGCTTGCTGACCACCTGGTGGACCGGC TGCCTGGGCCACGGGCACCCGCCAGCCCTGCAGCCATTCGAACCCTGATCCAGTGCTTGGGCAGCGTGGG CCGTCAGGCAGGCCATCGCCTGGGGGCCCACCTGGACCGCCTGGTGCCCATGGTGGAGGAGTTCTGCAAC CTGGATGACGATGAGCTAAGAGAGTCCTGCCTCCAGGCCTTTGAGGCCTTCCTAAGAAAGTGCCCCAAGG AGATGGACCCTCACGTACCCAATGTGACCAGCCTCTGCCTGCAGTACATGAAGCATGACCCCAACTATGA CCATGACAGTGATGACGAAGAGCAGATGGAGACCGAGGACAGTGAATTCAGTGAGCAAGAGAGTGAGGAC GAGTACAGTGATGATGACGACATGAGCTGGAAGGTACGCCGAGCTGCGGCCAAGTGCATGGCAGCCCTCA TCAGCTCTCGGCCCGACCTCCTGCCCGACTTCCACTGCACTCTGGCACCTGCGCTCATCCGCCGCTTCAA GGAGCGGGAGGAGAACGTCAAGGCAGACATCTTCGGGGCTTACATCATGTTGCTGCGACATACACGGCCC CCTAAAGGCTGGTTGGAGGCTGTGGAGGAGCCCACCCAGACTGGCAGAAACCTCAATATGCTGCGAGCAC AGGTGCCCCTAGTGATAAAGGCCCTGCAGCGACAACTTAAAGACCGGAATGTTCGGACCCGCCAAGGCTG CTTCAACCTCTTCACCGAGCTGGCAGGCGTCCTCCCTGGCAGCCTGGCAGAGCACATGGCTGTGTTGGTG TCAGGCATTGTCTTTTCACTGGCCGACTACTCAAGCTCCTCCACCATTCGGATGGATGCCCTGGCCTTCC TGCAGGGACTTCTGGGTACAGAGCCGGCTGAGGCCTTCCATCCCCACTTGCCTACCCTCCTACCACCTGT GATGGCCTGTGTGGCTGACCCTTTCTACAAGGTGGCAGCTGAGGCCTTACTGGTGCTCCAGGAGCTGGTG CGGACCCTGTGGCCACTGGATAGGCCTCGGCTGCTGGACCCTGAACCATATGTCGGAGAGATGTCCACAG CTACCCTGGCACGACTCCGAGCCACTGACCTGGACCAGGAGGTGAAGGAACGGGCCATCTCTTGTGTGGG CCACCTTGTGGGTCACCTTGGTGATCGACTTGGGGACGACCTAGAACCCACACTTATGCTTCTCCTGGAT CGCCTACGGAATGAGATCACCCGGTTGCCTGCTGTCAAGGCACTGACCCTGGTGGCCATGTCCCCATTGC GACTTGACCTGCAGCCCATCCTGGCTGAGGCCCTGCCCATCCTGGCTTCATTTCTGCGCAAGAATCAGCG GGCCCTGCGGCTGGCCACACTGGCTGCTCTGGATGCCCTGGCTCAGAGCCAGGGCCTCGGCCTGCCCCCG CCTGCCGTGCGGACTGTGCTAACCGAGCTGCCTGCTCTGGTCAGCGAGAATGACATGCATGTGGCCCAGC TGGCTGTCGACTTCCTCACCACAGTGACCCAGACGCAGCCCAGCTCTCTGGTTGAGGTCAGTGGCCCCGT TCTGGGGGAGCTGCTGCAGCTGCTGCACTCACCCTTGCTGCCTGCCGGGGTGTTGGCAGCCACTGAAGGC TTCCTGCAGGCGCTGGTGGGAACCCGCCCTCCGTGTGTGGAGTACTCGGAGCTCATTAGCCTGCTCACTG CACCTGTTTACAACCAGGTTGGGGACGGGGGACCCGGCCTGCACAAGCAGGTGTTCCACTCGCTGGCCCG GTGTGTAGCTGCCCTCTCAGCTGCCTGCCCCCAGGAGGCCGCAGGTACTGCCAGCCGCCTGGTTTGTGAT GCCAAGTCGCCCCACTCAAGCACGGGGGTCAAAGTCTTGGCATTCCTGTCACTGGCTGAGGTGGGCCAGG TGGCAGGGCCAGGCCCCCAGAGGGAGCTGAAGACCGTGCTTCTGGAAGCCCTGGGGTCTCCCAGTGAGGA CGTGAGGGCTGCAGCCGCATATGCCCTGGGCCGTGTGGGTGCTGGCAACCTGCCTGACTTCCTGCCCTTC CTGCTGGCACAAATTGAGGCCCAGCCCCGGCGACAGTACCTGCTGCTACATGCACTCAGGGAGGCCCTGG GGGCTGCGCAGCCTGACAATCTGAAGCCCTATGTTGAGGATGTGTGGGCGCTGCTCTTCCAGCGCTGTGA GAGCCCCGAGGAAGGCACTCGGTGTGTGGTGGCGGAGTGCATCGGGAAGCTAGTGTTTGTGAACCCTCCC TACCTCCTGCCTCGATTCCGGAAGCAGCTCGCTGCAGGTCAGCCATACACCCGGAGCACGGTCATCACTG CAGTCAAGTTCCTCATCTCAGACCAGCCTCACTCCATCGACCCTCTCCTGAAGAGCTTCATTGCGGAGTT CATGGAAAGCCTGCAGGACCCAGACCTGAACGTGCGCCGGGCCACACTCACCTTCTTCAACTCAGCTGTG CACAACAAACCATCACTGGTCCGGGACCTGTTGGATGACATCCTGCCCCTCCTCTACCAGGAGACCAAGA TCCGCCGGGACCTCATCCGAGAGGTGGAGATGGGGCCCTTCAAGCATACTGTGGATGATGGGCTGGATGT GAGGAAGGCTGCCTTCGAGTGCATGTACTCGCTGCTGGAGAGCTGCCTGGGCCAGCTGGACATGTGCGAG TTTCTGAACCACGTGGAGGATGGGCTGAAGGACCATTATGACATCCGGATGCTGACCTTCTTCATGTTGG CCCGACTGGCCACTCTTTGTCCTGCACCTGTCCTGCAGAGGGTGGACCGGCTCATCGAGCCACTCAGAGC CACCTGTACTGCCAAGGTCAAGGCTGGTTCTGTGAAGCAGGAGCTGGAGAAGCAGGAGGAGCTGAAGCGC TCAGCAATGCGGGCAGTGGCCGCCCTGCTGACCAACCCTGAGGTGCGCAAGAGCCCCACTGTAGCTGACT TTTCCGCCCAGATCCGATCCAACCCAGAACTCACCACCCTCTTCGAGAGCATCCAAAAGGACACAGCCTC CGGCCCCAGCACGGACTCTATGGAACTCAGCTAGCTGCCCCCAACACAAGTCTCCCTTCTTATCCCAATA GGCCTTGGCCTCTTCTGTCCTTCCCTGTCAGCCTCAGTGCCTTTGCTAGGGCCCCCTGCTCACAGCCCCA GTGCCAAAGCCGGGCCCACCAACTCAGGACGTTCATTCACGAGCGTCATGTCCCACCACCTTTACACTGC ACCCCTCCACTCACTTAGGAGCCAGAATTGGCCTATGCCCAAGACCAGAAGGCAAGCAGGGGGTTGACTT CCTACTTCACTTACTCTGAAGTCCAAATGTCAGTTAGCACCCTGAACCTAGCCATGGGTAGGGTATGTAG AGCCCATACAAGAGAACCAATTTATACTTAGTGCCTCATTAGTTAGCAGAGTGAGAACTGTTTACAGGGA AGATAGTGTGGCTCATTGGCCAGGTGTAGGTCCACTGTCTCAAACATCCCTAATCCCATCACCCAGGAGT GTAACAGTCGGCTTGTGCTCTTCCCGACTTTGTAGTTAGTACTGAAAATTTAAGTATAAATGACAGGTTT TGATTTTTAAGACATTGTTTCTCTTTGTAGCCACATATGGCCCAGACTCAAATCCACCTGCCTGGAATTA AAGCCAGCTGAGGCCAGTTATCTGAGTTAATAACATATACAAAGCTCTCCTCACAGGATTCTTATCCATA TTGATTCAGTCCTGGAAATAACAAGGCCGATGGCATAGACCTGTTATCCTAATTCCTTAGGAGGCTGAGG CTGGACCACACACTCAGGTCGTAGTGAACAAATTTAAGATCTACCTAAAAGCAACAGGGCAGGATAGCTC AGTACCACGCCCTCCTGGTAGCAACAACTTTCTAACCTGTCCTAAAGGACCCTATGCCTTCGGGCAGACA TATATACAGGCAAAACACCAAAGTTTAAAATAGCTCAGTTGTAGAGCACTCTCCAACACTACAGCAAGAT GATAAAACCTCATTTCTACAATGAGAACAAAAGCTTAGTAATAAAAAGGCTGGGATGTCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6824847 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6824847 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12443 same embryo
	EMAGE:12441 same embryo
	EMAGE:12442 same embryo
	EMAGE:12440 same embryo
	EurExpress:euxassay_011212 same experiment
	MGI:4823611 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source