EMAGE mouse gene expression spatial database

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EMAGE:12441

Pcmtd1 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 ( MGI:2441773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011211. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011211_01	euxassay_011211_02	euxassay_011211_03	euxassay_011211_04

euxassay_011211_05	euxassay_011211_06	euxassay_011211_07	euxassay_011211_08	euxassay_011211_09

euxassay_011211_10	euxassay_011211_11	euxassay_011211_12	euxassay_011211_13	euxassay_011211_14

euxassay_011211_15	euxassay_011211_16	euxassay_011211_17	euxassay_011211_18	euxassay_011211_19

euxassay_011211_20	euxassay_011211_21	euxassay_011211_22	euxassay_011211_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12441_wholemount_strong.wlz

12441_wholemount_moderate.wlz

12441_wholemount_weak.wlz

12441_wholemount_possible.wlz

12441_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12441_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 16 17 18

thyroid gland

moderate

regional

moderate expression: see section 10 11 15

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

weak

regional

weak expression: see section 05 06 07 08 19 20 21

hindbrain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

spinal cord

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 16 17

neural retina

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 22 23

lower jaw incisor

moderate

regional

moderate expression: see section 16 weak expression: see section 11 15

lower jaw molar

moderate

regional

moderate expression: see section 07 18 weak expression: see section 06

upper jaw incisor

moderate

regional

moderate expression: see section 16 weak expression: see section 11 15

upper jaw molar

moderate

regional

moderate expression: see section 18 weak expression: see section 06

testis

moderate

regional

moderate expression: see section 06 07 08 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9224
Entity Detected:	Pcmtd1, protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 ( MGI:2441773)
Sequence:	sense strand is shown >T9224 CTTCTGCGCCTTCGGTTTAATTTATTTTTGGAAATCAAGTTCAATATTAAAAAGAAAGAGCACACATTAT TGGAAGCCATTTCTACTAATTCGTTACATTTTAACTTATGATTTGATTTGAATACTGTCATGGGAGGAGC TGTGAGTGCTGGGGAAGATAATGATGACTTAATTGATAACCTTAAAGAAGCTCAGTACATCCGAACTGAA AGAGTAGAGCAAGCCTTCAGAGCCATTGACCGCGGAGATTACTATCTGGAAGGCTACCGAGACAATGCTT ACAAAGACTTAGCCTGGAAGCACGGGAACATACACTTGTCAGCACCTTGCATTTATTCTGAAGTTATGGA AGCTTTGAAACTTCAACCAGGATTATCTTTTCTTAACCTGGGAAGTGGAACCGGATATTTAAGTACAATG GTGGGCTTAATTTTAGGTCCTTTTGGAATAAATCATGGGATTGAACTTCATTCAGATGTGGTGGAGTATG CCAAGGAAAAACTGGAGAGCTTCATCAAAAATAGTGATAGCTTTGATAAATTTGAATTCTGTGAACCCGC ATTTGTGGTGGGTAATTGCCTTCAGATAGCTTCTGACAGTCATCAGTATGATCGAATTTACTGTGGAGCT GGAGTCCAGAAAGACCACGAAAACTACATGAAGATCTTACTCAAAGTTGGGGGCATATTGGTCATGCCTA TAGAGGATCAGTTGACACAGATTATGCGGACTGGACAAAACACTTGGGAAAGTAAAAATATCCTTGCGGT TTCCTTTGCACCACTAGTACAGCCTAGTAAGAATGATAATGGCACACCGGACTCTGTGGGGCTCCCTCCA TGTGCTGTCAGGAATCTCCAAGACTTGGCTCGCATTTACATTCGACGTACACTTAGAAACTTCATAAATG ATGAGATGCAGGCCAAGGGGATTCCACAAAGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAG AATTAATACTTACGTATTTGTGGGTAATCAGCTTATCCCTCAGCCTCTAGACAGTGAAGAGGATGAAAAA ATGGAAGAAGACAGCAAAGAAGAAGAGGAGAAAGAGCACATCGAAGCCATGAAGCGCGAGGAACCACCTC AGAACCTACTGAGGGAAAAGATAATGAAGCTGCCACTCCCAGAGTCTTTAAAGGCTTACTTGACATATTT TCGAGACAAATAACTTAAACAAGGAAGAATGCCTACTGATAAACTCCCTTACTCTTGTAAATGTAGTATT AATTAGGACTTAGAGAATTACTTCATTAGAATGAATTATTGCAGAAAAAAGTCTAAGTTATTTCTTTTAA TAACAGAAATGATGTATTCAGTGATTTAAAAGAGTCTCTTTCCTAAGATCTATTTTTCTTAAATCTTGAA GAATTACTGTTTTGATCTCAAAGTGGGATGGGTCAATAGTAAGAACTTTGTATACAGTACTCTTTCTGGT TCTTCATGGATTTGTAGTGTTGGCAATTGAATTTCATTCTATAAGAGGTTGATACACATATTGTCACACA TATAGGCTTATTTAAATTGCCGTTTGCATTTCTGTTACTTGAGCTTGCTTATCTAATAAGGAAAGCAAAT GCTTGTAGACCTACTTACCTTACTTATGTTCTAGACATATTCCGGGCAACGTATGAGTGCAATACTAATT TAAGATACTGAGTAATTTAGCTTTAAAAACCACAGACTTCATGAAATTTTACAGACCCGAAACTTAAGCT TTTGAATCTATTGCCAAAAGGCATGGGGAAAATTTCTGCTTCTCTCATAGAGATTTTTAAGAGCTAGTGA ATGTTAAAGTAGGAAGTGGCTACTTGACACAACTAGTTTGTATGGGCCCTTTGTGTTCTCAGTATTTAAA GATAATGAGGTTATCTTAACTATAGAATTTTAAGCGGTAGTATATTTAGGATTGCTTTTCTATAAATAAC TATAAAGTATTAGATTTGGAAAGTCATTCACTGTCCCATTTGCTTCTAAATGCAGACTGTGAATAAGTCA CATTAAGTGATCAGCACTGCTTAGGGACCTCCAGCAGCAGGGGGAATGATATGATCAGTAGAGACATTTT AGCAGTGGAACTTTTCATTAAGTAGATATTTATCTTTGACAAGGTAGGCCACACTGGCCTCATCTGCTCT GAATCTTTCTAATATAAATAGTCATGGGAGTTGTATGTAGATGCGTTTGTGTTAAAACTTGCTTCAGAAT CAGAGTGGGATCTCTGGGACCTATACCAAACTCATTATCCTTCATTCTGTATTTTGTTTCTTTGATAGAA TGATGATGATTTTCACTTTTTGCTTAATACTACATTCTTAAGCTACTTACATAGGGTATCAGTGATACAC CAGGCAGTGCATGTCATTACTAGTTCTTTCTTCCTGGTAGGGCCTGCACAGTTATTCCTGGTTAAGAAGC TGTGGGATGTGACCTACTTACTGCTTACCATCACACGGAAGGGAAGATTTCAAGTGTCTCACTTATTCCC ACCACATCACTGTCAGAGCACCCTCTCGGATTTACATAAGGAAGTACAATCATGATGATTCAAATAGTTT ATAACTCAGGTCCAATAATTCTGTGTAGGTGATCAGTTAAAACAGTTTAAAGATGAAATTGACCCAAATA ATTGATAATGTTTTAATAATGTGAACAACAGTGTTATGGAAAGGATTCGTTTGACTCTTGTGAAATGGGC CTGTATGATTTTTCTTAATGTAATTAGTAAACAAACTATTATTAAGATATGCTCTATCCTGTTTCTGTCC AAAAAAGAAAAAAGACATCTCCCTCTCTCTGACGTGGTACTGGGGGTTAAACCCAGGACCAGGACCTCTT GCATGCTAGGCAAGCACTCTACAAATGAGATATATCCCTAGACCAACATACTCTTTTATTCTTTTTAAAA TTTTGTTTAAGAAGTTTAATCTTGAACTCCTGATACCCACATAGTCTTATCTGGGAAGAGTTGTCATTTA TTTTCCATTCTGTCACCCACATCTCTTTACTTTTCTCTTAAGAGCTTGATGTACAGAAAACTGAAAATAT GTAATGCTCAGTGATCTTATTATTTAGGAGTGTTTTGACTGATATATTTAAAGATAGATTATATTCATGC TGTCTTCTGTTGACTTTGAGAAATTGTACAGTAGATTGTCTTGATCATTGCATTTGTTGTCTTTGAAATG TATATTTTGTCATTTTAAATTCATTTACTTTTCAGGTGTGACTTTATGGTTGACTTTTAAAGGTAGGGAT AGATGAAAATTTAAGGAAAGCTATCTTATCTATCAACTCGGATGCATTAGATTAAGAATATTTGGGTTCG GGCTTATTTTTATTTGAGTGTGGAAAGTTATGCTTTATAAGTAAAGAAGTAGCAAATACAGATGAAATAA AAATAAATGGAAGAATATAAAGAAGAAAAAACTAAGAAAATAAACAAAAATGTAAGTGAAAGTAAGCCAT ATGTGATGGCTACTGCCTGTGGTCAGGGCTTGAGAAGCTGAGGCAGGAGGATTACAAATTTAAGGCATCT GTGGTTTCAGAGGGACTTTGAAGCCCATCTGGACCACAGAGTAAGACCGGCTTTTGTTCTTCAGAGAACA TAAACAAAAGAAAGTATATGATAATTATGGGGAATAGTCTCGTGGATTTAAATATAGACAAAAATACATT TAAGTTTATCTTTTGAAGACAACAAAGAGGGCTTGAATGGATGTGTGTGGGGCTTAAGTCATGCTGCAGA TTGGAGACAGGGCCTGAAGGAACATCGACCATCACTGAAATCCCCAGGATCTGGGAGCAACACCATAGCT GCCAAGGGTCTAATTTAAATCTGTCCTACTCACTGGTCAACATATTGGACTCTGGTGTTAATATAATGTT AAAATAGCTGCTTACATGGTTAAAAGAAAAGAAAAGAAAATGTGCTGATTGTTTGAGATGAAAGTAAAAG AAAACCTGCTAAATCATTGGGGAACTTGGGAGGAAAATCATTTTAAATAAACAAATCACATCATTACACA GCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833458 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833458 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12443 same embryo
	EMAGE:12439 same embryo
	EMAGE:12442 same embryo
	EMAGE:12440 same embryo
	EurExpress:euxassay_011211 same experiment
	MGI:4827096 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source