EMAGE mouse gene expression spatial database

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EMAGE:12539

Rnft2 ring finger protein, transmembrane 2 ( MGI:2442859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011273_01	euxassay_011273_02	euxassay_011273_03	euxassay_011273_04

euxassay_011273_05	euxassay_011273_06	euxassay_011273_07	euxassay_011273_08	euxassay_011273_09

euxassay_011273_10	euxassay_011273_11	euxassay_011273_12	euxassay_011273_13	euxassay_011273_14

euxassay_011273_15	euxassay_011273_16	euxassay_011273_17	euxassay_011273_18	euxassay_011273_19

euxassay_011273_20	euxassay_011273_21	euxassay_011273_22	euxassay_011273_23	euxassay_011273_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12539_wholemount_strong.wlz

12539_wholemount_moderate.wlz

12539_wholemount_weak.wlz

12539_wholemount_possible.wlz

12539_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12539_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9164
Entity Detected:	Rnft2, ring finger protein, transmembrane 2 ( MGI:2442859)
Sequence:	sense strand is shown >T9164 CTGCAGTGTCTGGCAGGTCTGCTGTCTCCAATGAGTCTCCTGTCCTGTGCTTCCCTCTGAAGAGGAGGAA GAGGAGGAGGAGGAGGCCTGCTCTCTCTGGGACCCAGTGGCCACATCCCATCAGGAACCCTGAGTGTCCA CGGCACATTTCGTCAGCATGGAGTTCTGAGGTCCATGTGGCTCCTTGCTGCGCACCAGGTGCTAAGGAAA ATGCAGAGACGCCACAGTAGCAACACGGACAACATTCCACCTGAAAGAAGTCGCAGCCAGGCGCTCAGCC CCGAGGCCAGCGTGGATGAGGGTGGTGTATTTGAGAGTCTGAAGGCAGAGACGGCCTCCCCTCCTGCGCT CTTCTCAGGCCTGGCAGGTGGCCTGCCTGCCAGCCCCTTCCCAGCAGGCCTGGTGCTGGGCTCCACGGCG GGTGGCGGGGATGTCTTCATCCCCATGCCAGCCACCAGGGATGAGGCCGGTGGCCGCAGCGCCGAAGGAA GCACCTACCACCACCGCCAGGCCCACCACCACTTCCACCATGGTGCCCACCGCGGGGGCTCCCTGCTGCA GCACGTGGGTGGGGACCACCGTGGACACTCAGAGGAAGGGGTCGACGAGCAGCCCGGAACTCCAGCACCC GCACTGTCTGAGCTCAAGGCTGTGATCTCCTGGCTTCAGAAAGGACTGCCCTTTATCCTCATCCTGCTGG CCAAACTGTGCTTCCAGCACAAGCTGGGCATTGCTGTGTGCATCGGGATGGCCAGCACCTTTGCCTATGC CAACTCCACCCTCCGGGAGCAGGTGTCCTTGAAGGAGAAGAGGTCGGTTCTGGTCATCTTGTGGATTCTG GCCTTCCTGGCAGGGAATACCATGTACGTCCTTTATACCTTCAGCTCCCAGCAGCTCTACAGCAGCCTCA TATTCCTGAAGCCCAACCTGGAGACACTGGACTTCTTTGACCTGCTGTGGATCGTGGGGATCGCCGACTT TGTACTGAAGTACATCACCATCGCCCTCAAGTGCCTCATCGTGGCCCTGCCCAAGATCATCCTGGCTGTC AAATCCAAGGGAAAGTTTTATCTGGTCATTGAGGAGCTGAGCCAGCTGTTCAGGTCTCTTGTCCCCATCC AGCTGTGGTACAAGTACATCATGGGGGACGACTCCTCCAACAGCTACTTCCTGGGAGGAGTCCTGATCGT CCTCTACAGTCTCTGCAAGTCGTTCGACATCTGTGGTCGCGTGGGCGGACTCAGGAAAGCATTGAAGCTC CTTTGCACCTCACAGAATTACGGGGTCCGAGCCACAGGGCAGCAGTGCACGGAGGCTGGCGCCGTCTGCG CCATCTGCCAAGCTGAGTTCCGAGATCCCATGATTCTCCTGTGCCAGCACGTGTTCTGTGAAGAGTGCCT CTGCCTGTGGCTGGACAGGGAACGCACCTGCCCGCTCTGCCGCTCGGTTGCTGTGGATACCCTGCGATGC TGGAAGGACGGGGCCACGTCTGCACACTTGCAGGTGTATTGAGCTAAACATGAGTGTCTGGGGAGCCCAG CCCCAAGGATGCCTGGGAAACAGCCTCCAGGACTTTCTCCTGCCTCCCACCACCTCTATCTGATGACCCC AGGGCCTCCACACCAACACTTCCTCAGCGCCCCAGTACAGTGTGTGTGTCCTACCTCACCAGAAAGGGGA GCCCTCCTCACTCAGACTTGGCTCCCCCACAGGTAGGTGATCCGTCCCCTCTAAGTGGAGGAGTTACCCA TACATACACAGTCTCCCTTCATTTAACCTGCTTCCAGCCGCTGGTGCTCAATGCTACGAACTAGTATGAG CTCAGAGAGTACAGTCTGACCCAGGTCTGCACACAGGAGCCGATGGGAGAACGTCCAACATGTGAGCACC AGGCTTCACCCACCTAGCTGACCTTCAAAACAGCAGGATGCACCAAGCATCCCGCTCATGCCGCCTCCTG GCATCTCCACATCAGCCCTAGGGTGGGGCTACTTGCTCTCTCTCACACACACACCATTGGCAGGTGAGAA GCTTGGGGTGGCCGCCCAGCTAGTAAGGGGCCAATGTGTCTCCAGCGCTGCCTGGTGCTGTTGCTGCAAG TGCCCCTCGCTGGCTGAGCATCCGTGGCCTGGGCATACAGCACATCAGAGACAGACTGCTCTGAGCAGGC AGGCAGCGAGGTCAAATGGCAGGAGCCATGGACAGACTGCACCTTTGTGATTCCTTTTCTCCCGGGATCC ACCTGGGACAGTCAGTCTCAGAAGGGTCAGCTCTGGTCCAGAGTGTATGCCACCTGCCTTCTGTCATTCT TCTGTCCTTATTCTCATCTACATTGTCCCTCACTGACAGCTCAGGCCTGTGCAGAGCTGTGGACATGGCT ACCTCCCCCCCCCACCCCCCGGAGGCTTCAATCCATGTCATAAGTGGCACACGGCTTCGGAGTGATCCAT GTGTCGTCCCCCCTCCCCCCCCCCCTCTCTAGTGAGGTAATTAGCTCGGAGAAACAACAGTCCTTCCAGA GTCTCTGAAAAGACTGCTTTATATCTTTAACTAACCGCCCGAGCGAGCGGGAACGCTGGGAGCAGATGGC AGGTGTGCTTTCACATCCTTCGGTCTCTAGGACACAGCTTCTTCTCCCCGCGGGAAACTCTCTGATTGGA AACCTGGGACGAAATAGCACAGGTGACCGCACAGAAGCCTCTGGAATTCTGCCTGCCTCCACTGGCTCCT GACTCGGTTCTCTCACAGCCGGCATCCGCACAACAGGCTGGCCCAAGCCACGTTTGAGATTAGCACCAAG GGGGTTGGGGGAGGGAGAAAGGAGCCTCTGTAGATTCAGGGTGCTCTCTCCTCCCCTTTGTAAATTTAGG ACCCATGCCTTGGGCACCCCTGCTGGGACTTCTTCCTCTGCTTGGTCTCCAGGGCACTTTCCTGGTACCA GTAACACACCCACCCTTTCCAGGAGACCAGGGCATCCTTCAAGCTTTGTTACTATGGCAATGGCCATGAT GCTAAGCAATCCCACTGCCTGGATGTCCTAAGAGGGAAGGGAGGATGGGCCCTTCTGTCCTCTCTGGTTT GAGGGGGTACCCAGGAGAGTGGTGGGACTGCCTGCAACTGGTTTTTATCAGAGAGATTGCTTTGCCTGAG ATGCTGTCAGCATTGTGGAGGGGTGGGAGCTGCAGGAGGTGCACGGAAGTGTAGGGTGGGATGGATCAGA TGCAGCCACTGGGAGTCGGGCTTGGAAACCTGACTCCAAACTTTGTGATGTCCCTATACCCTGTATCCCC CCACCCTCCATGGAGCTACAAAACCTAGCATGTTTCTTGCTAGGACTGAGAAAACTAACAGCAGGACTGA AGATGTAGCTCAGTCGGTAGAGCACTTGCCTAGTGTGCAGGAAGCCCTGAGTTCCGTGGCCAGCACCACA TGAACTAGGCATGGCAGTGCACAACTGAATTGGCAGCGCTGGGTGGGAGAGGCAGGAGGATTAGAAGGAC AAGATCATTCCTTGGCCACATAGCGAGTCCCAGGGTAGCCTGGGCTCTGTGAGACTCCATCTTAAAAGGG AGAACAGGTGAACTAACAGTGTGGGTTACTGGAGAGTGTGCCAAGGGTTTTACACACCCCCTCCATTACT TCTCTGACATCTTGTAAATATAATTATATCCCTACCACACATGGAGCATCCCCTGGGACAGTGAGATGCT CTTCAGTTCCCATCCTTCCAGGGAACACCTCAGATGGCCAGTGGGGTTCATCCAGTTTAGAGGGCTCAGG CAGTTTCCCAGACAGCAGGCTGTGGGAAAGTGTCCCCTGCAGTGGCGTATCAGTCCTTTGCTTTGAGCAG GTCAGTGGAGCATCAGGGGCCGGATATGGCTCCCGGTATATTGTGGGTCACAGACTCAAATCCCTCTGAG TCCTCCAAGTCTTGTGTGTGTGAGGACCTGAGATTAACAAGGTAGGGGAGGCTGGTGAGAGGGCAGAGGT TGAGAGTGCTTGGCTTGCATCTTTAAGAGGACCCAGGACTCACACCAAAGGGCTCACAACTCCAGCTCCA GGGACACACACATCATTTAAAACAAATCTTCAAGAACCATGTGGGAAGAAGAACGGCCTGCTTTGGACCC GAGCCTAACAGGCCCAGCCTTCCAAAGGGAACGGCACAGGTACGGAGCCTTGGGACCATCCCCCACTGCT GAAGCGGAAGTGGGCAGCGACCTGAGGTCCTCTGCTTTTGCTTTGGGACATGTCTTTGGTGTCTTTGAGA ACCTTCTGCCAATAAACCTCCCCCATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827287 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827287 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12542 same embryo
	EMAGE:12541 same embryo
	EMAGE:12540 same embryo
	EMAGE:12543 same embryo
	EMAGE:12538 same embryo
	EurExpress:euxassay_011273 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source