EMAGE mouse gene expression spatial database

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EMAGE:12540

Dhx34 DEAH (Asp-Glu-Ala-His) box polypeptide 34 ( MGI:1918973)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011244. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011244_01	euxassay_011244_02	euxassay_011244_03	euxassay_011244_04

euxassay_011244_05	euxassay_011244_06	euxassay_011244_07	euxassay_011244_08	euxassay_011244_09

euxassay_011244_10	euxassay_011244_11	euxassay_011244_12	euxassay_011244_13	euxassay_011244_14

euxassay_011244_15	euxassay_011244_16	euxassay_011244_17	euxassay_011244_18	euxassay_011244_19

euxassay_011244_20	euxassay_011244_21	euxassay_011244_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12540_wholemount_strong.wlz

12540_wholemount_moderate.wlz

12540_wholemount_weak.wlz

12540_wholemount_possible.wlz

12540_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12540_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 06 07 08 09 12 13 weak expression: see section 05 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9382
Entity Detected:	Dhx34, DEAH (Asp-Glu-Ala-His) box polypeptide 34 ( MGI:1918973)
Sequence:	sense strand is shown >T9382 GGGGCGGGGCGTGTGTGCGGGGTGTCTGCGTGGCTTTGGTCCCTGGTGCAGGATTTGTGTGGGGCTATAG TTCCGAGCGAACAGGGGAGTCAAGAGAACCCCAATTTTAATGAAGGAATGTGCCAGGACCCAGAGAGCTC TGGCGGTGCGCGATTCTTAGAAGCTGGAGGACCAAGAGGCCCGGAACTGAAACCACAGAGGGAACAAGTT TCTGAGAAAGGGAGAGAAAAGGGGACCAGGGTGATGGGGTGCCTGCAAGAGAGGCTCACAAGGGCGCCAG GAAGAGATTGGCAAGAAAGCCTGGATGGTGAACCTGGAAGTCCAGAATGGACCCTTGTCCCTTCCAGAGA CCACTGCAGGAGTCATGAAGCGCTTTGTGCAGCTCCACCTACCCACCAGGCCCCTGGCTACCCACCCTGC CGTCCACAGAGCCTCTGTACATGGAAAGGGGAGGTGACTCTTTGAAGAGAAAGCAGTTCCTGGGGTCGGG AGTTAGGTGAGAATACTTAGTCTAAGCGATCAGAACGGAGACTGTTTCTGTGACAGCAGTTTGATAGGAA CCATGCCTCCTCCCAGGACAAGGGAGGGGAGGGGTCACCGAGACCGAGACCACCATCGAGCTCCCAGGGA GGAAGAGGCCCCGGAGAAATGGGACTGGAATTGTCCAGAGACTCGTTGTCTCTTGGAAGATGTATTCTTC CGCGATGAGGATTATATCCGTCGTGGTTCAGAGGAGTGTCAGAAGTTCTGGGCTTTCTTTGAACGCCTGC AGAGATTCCAGCACCTCAAGACCTCCCAGAAGAAGGAGAAGGACCCTGGCATGCCCAAACATGGCATCGC TGCTTTAGCTGACCTACCTCTCACTTACGACCCACGCTACCGCATCAACCTCTCCATTCTCAGCCCAGAC ACTCGGGGTCGTCATGGGCCCGGCAGGGGGCTGCCGCCCGAGAGAGTGTCCGAGTTCCGCCGTGCATTGC TTCACTACCTAGACTTCCAGCAGAAGCAGGCTTTTGGGAGGCTCGTCAAACTGCAGCGTGAGCGAGCGGC ACTTCCCATCGCCCAGTATGGGAACCGCATCCTGCAGACTCTCAAGGAACACCAGGTGGTGGTGGTGGCC GGGGACACAGGCTGTGGCAAGTCTACTCAGGTGCCCCAGTACTTGCTGGCCGCCGGCTTCAGTCACGTGG CATGCACTCAGCCCCGGAGAATCGCCTGCATCTCACTGGCCAAGCGCGTTGGCTTCGAGAGCCTCAGTCA GTATGGCTCCCAGGTTGGCTACCAGATCCGCTTTGAAAGCACTCGGTCGGCAGCCACCAAGATCGTGTTC CTGACGGTGGGGCTGCTCCTACGCCAGATCCAGCGTGAGCCCAGCCTGCCCCAGTACCAGGTCCTGATCG TGGACGAGGTCCACGAGCGACACCTGCACAATGACTTCCTGCTGGGTGTCCTGCAGCGCCTGCTGCCCCA GCGGCCTGACCTCAAGGTCATCCTCATGTCGGCCACCATCAACATCTCTCTCTTCTCCAGCTACTTCAGC CATGCTCCGGTGGTGCAGGTGCCTGGGAGGCTCTTCCCCATCACGGTAGTCTACCAGCCGCAGGAGGCAG ACCAGACAGCATCCAAGTCAGAGAAGCTGGACCCACGGCCCTTCCTGAGGGTGCTGGAGGCCATCAACAA TAAGTATCCCCCCGAGGAGCGGGGGGACCTTCTTGTCTTCCTCAGTGGCATGGCGGAGATCACCACGGTG CTGGACGCAGCCCAGGCTTATGCCAGCCTCACCCAGCGCTGGGTGGTGCTGCCGCTGCACAGCGCCCTCT CTGTGTCTGACCAGGACAAGGTGTTCGATGTGGCTCCAGCTGGAGTCCGGAAGTGCATCCTGTCCACCAA CATTGCCGAGACCTCAGTCACGATCGATGGCATCCGCTTCGTGGTGGATTCTGGGAAGGTGAAGGAGATG AGCTACGACCCACAGGCCAAACTGCAGCGGCTGCAGGAATTCTGGATCAGCCAGGCCAGTGCTGAGCAGC GCAAGGGCCGAGCGGGCCGCACGGGCCCCGGCGTCTGCTACCGTCTCTATGCCGAGTCTGACTATGATGC CTTTGCGCCGTACCCAGTCCCGGAAATCCGCAGGGTGGCCCTGGATGCACTGGTGCTTCAGATGAAGAGT ATGAGTGTGGGGGACCCACGCACCTTCCCCTTCATCGAGCCCCCTCCACCTGCCAGCGTGGAAACCGCTA TCCTCTATCTCCAAGAGCAGGGGGCCCTGGACAGCTCGGAGGCCCTCACCCCCATCGGGTCCCTGCTGGC CCAGCTGCCTGTAGATGTTGTAATTGGGAAGATGCTGATCCTCGGCTCCATGTTCAGCCTGGCAGAGCCT GTGCTCACCATTGCTGCTGCCCTCAGCGTCCAGTCACCATTCACCCGCAGTGCCCAGAGCAACCTGGACT GTGCCACTGCCCGGAGACCGCTGGAAAGTGACCAGGGTGACCCCTTCACACTCTTCAATGTCTTCAATGC CTGGGTGCAGGTGAAGTCTGAGCGGAGCGGAAACTCTCGAAAGTGGTGCCGCCGCCGGGGTGTGGAGGAG CACCGGCTGTATGAGATGGCCAACCTGCGGCGCCAGTTCAAGGAGCTGTTGGAGGATCATGGATTGCTGA GTGGGGCCCAGGTAGTGGCCCCAGGGGACAGCTACAGTCGGCTGCAGCAGCGCCGAGAGCGCAGGGCCCT GCACCAGCTGAAGCGACAGCATGAGGAGGGTGGGGGACGCAGGCGCAAGGTGCTGAGGCTGCAGGAGGAT GGCTGCTCCAGCGATGAAGAGGACCGAGAGGGCTCTACCTCCCAGAGGGCTGACAGCGTTGACATCCAGG ATGTGAAATTCAAGCTTCGGCACAACCTGGAGCAGCTGCAGGCAGCTGCCAGCTCAGCCCAGGACCTGAC CCGAGACCAACTGGCCCTGCTCAAGCTGGTGCTCGGGCGGGGGCTGTACCCACAGCTGGCTGTGCCCGAT GCGTTCAACAGTGGCCGCAAGGACTCAGACCAGATTTTCCACACGCAGGCCAAGCAGGGCACCGTGCTGC ACCCCACCTGTGTCTTTGCCAACAGCCCTGAGGTATTACACACACAGGGGCAGGAGGCCTCAGGCCAAGA AGGGAGCCAAGATGGCAGGGACCAAATGAGCTGCAAGCATCAGCTGCTGGCCTTCGTCTCCCTACTGGAG ACCAACAAGCCATACCTGGTGAACTGCGTCAGAATCCCGGCCCTCCAGTCCCTCCTGCTGTTCAGCCGCT CCATTGACACCAACGGTGACTGCTCCCGCCTGGTGGCTGACGGTTGGCTAGAGCTGCAGCTCGCAGATAG CGAGAGTGCCGTCCGGCTCCTGGCAACATCCCTGCGGCTCCGTGTCCACTGGGAAAGTGCCCTGGACCGG CAGCTGGCCCGCCAGGCCCAGCGACGGAAGCTAGAGCAGGAGGAAGACATGGGGTCTCCAGCAGTCAGCC CCCAGGAGGTGGCTGCTCTGAGCAGGGAGCTGCTGCAGTTCATGGTGGCCAAGGTTCCCTACCGCCTCCG AAGGCTCACTGGTCTAGAAGCCCAGAACCTCTACGTGGGACCCCAGACCATCACGACCGCCCCTAGTCTG CCCGGCCTCTTTGGAAATTCTACCCTGTCTCCACACCCCACCAAGGGGGGCTACGCTGTCTCTGATTATC TCACCTACAACTGCCTCACGAGTGACACTGACCTGTACAGTGACTGTCTGCGTTCCTTCTGGACCTGCCC ACACTGTGGCCTGCACATGCCCTTCACACCCTTGGAGCGCATTGCTCATGAGAACACCTGCCCTGAGGCG CCAGGCGATGATCCAGGGTCTGAGGAGGCTGCTCCTGCACCACCACAGAAGACATCAGCCCTGCAGAGGC CCTACCACTGCCAGGTGTGCGGGCAGGACTTCCTCTTTACCCCAACTGAGGTACTAAGACATCGAAGGCA GCATGTCTGAGCCAGCACTGCTCCTGGAGCGCCCAAGTGGGGGACAGCAGTGTGTCCATGCAAATAAAGT CAGGCTTTGCTTCAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854931 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854931 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12542 same embryo
	EMAGE:12541 same embryo
	EMAGE:12543 same embryo
	EMAGE:12538 same embryo
	EMAGE:12539 same embryo
	EurExpress:euxassay_011244 same experiment
	MGI:4824302 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source