EMAGE mouse gene expression spatial database

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EMAGE:12543

Serpina1e serine (or cysteine) peptidase inhibitor, clade A, member 1E ( MGI:891967)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011248. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011248_01	euxassay_011248_02	euxassay_011248_03	euxassay_011248_04

euxassay_011248_05	euxassay_011248_06	euxassay_011248_07	euxassay_011248_08	euxassay_011248_09

euxassay_011248_10	euxassay_011248_11	euxassay_011248_12	euxassay_011248_13	euxassay_011248_14

euxassay_011248_15	euxassay_011248_16	euxassay_011248_17	euxassay_011248_18	euxassay_011248_19

euxassay_011248_20	euxassay_011248_21	euxassay_011248_22	euxassay_011248_23	euxassay_011248_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12543_wholemount_strong.wlz

12543_wholemount_moderate.wlz

12543_wholemount_weak.wlz

12543_wholemount_possible.wlz

12543_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12543_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 06 07 08 09 12 13 moderate expression: see section 05

hindgut

strong

regional

strong expression: see section 10 11 12

rectum

strong

regional

strong expression: see section 10

midgut

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 moderate expression: see section 14 15

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

maturing glomerular tuft

strong

regional

strong expression: see section 16 moderate expression: see section 05 weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9947
Entity Detected:	Serpina1e, serine (or cysteine) peptidase inhibitor, clade A, member 1E ( MGI:891967)
Sequence:	sense strand is shown >T9947 ACGCAATGGGGTCCTCAGCCTCGGTCACCACCCAGCTCTGAGACAGCAAGCTGAAAATGACTCCCTCCAT CTCATGGTGTCTACTGCTTCTGGCAGGCCTGTGTTGCCTGGTCCCCAGCTTTCTGGCTGAGGATGTTCAG GAGACAGACACCTCCCAGAAGGATCAGTCCCCAGCCTCCCATGAGATTGCTACAAACCTGGGAGACTTTG CCATCAGTCTATACCGGGAGCTGGTCCATCAGTCCAACACTTCCAACATCTTCTTCTCCCCAGTGAGCAT TGCCACAGCCTTTGCTATGCTCTCCCTAGGGAGCAAGGGTGACACTCACACGCAGATCCTAGAGGGCCTG CAGTTCAACCTCACACAAACATCGGAGGCTGACATCCACAATTCCTTCCAACACCTCCTCCAAACCCTCA ACAGACCAGACAGTGAGCTGCAGCTGAGCACAGGCAATGGCCTCTTTGTCAACAATGACCTGAAGCTGGT GGAGAAGTTTCTGGAAGAGGCCAAGAACCATTATCAGGCAGAAGTCTTCTCTGTCAACTTTGCAGAGTCA GAGGAGGCCAAGAAAGTGATTAATGATTTTGTGGAGAAGGGAACCCAAGGAAAGATAGTTGAGGCTGTGA AAAAACTAGAACAAGACACAGTTTTCGTCCTGGCAAATTACATTCTCTTTAAAGGCAAATGGAAGAAGCC ATTCGATCCTGAGAACACTAAGCAAGCTGAGTTCCACGTGGACGAGTCCACCACGGTGAAGGTGCCCATG ATGACCCTCTCGGGCATGCTTGACGTGCACCATTGCAGCACGCTCTCCAGCTGGGTGCTGCTGATGGATT ACGCGGGCAACGCCACTGCTGTCTTCCTCCTGCCCGATGATGGGAAGATGCAGCATCTGGAGCAAACTCT CAACAAGGAGCTCATCTCTAAGTTCCTGCTAAACAGGCGCAGAAGGTTAGCCCAGATCCATATCCCCAGA CTGTCCATCTCTGGAAACTATAACTTGGAGACACTCATGAGTCCACTGGGCATCACCCGGATCTTCAACA GTGGGGCTGACCTCTCCGGAATCACAGAGGAGAATGCTCCCCTGAAGCTCAGCCAGGCTGTGCATAAGGC CGTGCTGACCATCGATGAGACAGGAACAGAAGCTGCAGCAGCTACAGTCTTACAAGGCGGTTTTTTGTCT ATGCCCCCTATCTTGCACTTCAACCGCCCTTTCCTTTTCATAATATTTGAAGAACACTCTCAGAGCCCCC TCTTTGTGGGAAAAGTGGTAGATCCCACACATAAATGACCACCCTAAGAAGTCATCCTTCCTTCTGAATT GGGTTCCTTTCCATTAAACACGGGCTGGCCTGGCTCATGCCTGATGCTACAGAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30381238 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30381238 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12542 same embryo
	EMAGE:12541 same embryo
	EMAGE:12540 same embryo
	EMAGE:12538 same embryo
	EMAGE:12539 same embryo
	EurExpress:euxassay_011248 same experiment
	MGI:4827978 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source