EMAGE mouse gene expression spatial database

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EMAGE:12626

Spty2d1 SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) ( MGI:2142062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011306. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011306_01	euxassay_011306_02	euxassay_011306_03	euxassay_011306_04

euxassay_011306_05	euxassay_011306_06	euxassay_011306_07	euxassay_011306_08	euxassay_011306_09

euxassay_011306_10	euxassay_011306_11	euxassay_011306_12	euxassay_011306_13	euxassay_011306_14

euxassay_011306_15	euxassay_011306_16	euxassay_011306_17	euxassay_011306_18	euxassay_011306_19

euxassay_011306_20	euxassay_011306_21	euxassay_011306_22	euxassay_011306_23	euxassay_011306_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12626_wholemount_strong.wlz

12626_wholemount_moderate.wlz

12626_wholemount_weak.wlz

12626_wholemount_possible.wlz

12626_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12626_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9241
Entity Detected:	Spty2d1, SPT2, Suppressor of Ty, domain containing 1 (S. cerevisiae) ( MGI:2142062)
Sequence:	sense strand is shown >T9241 CTCAGCGAGAGGCAAGACTCGCATCCACCGCGTCCCGAGTCCCCGGCCCAGCCGGGCTTTTCTCTTCGGC CAGACATGGACTTCAGGGAAATCCTCTTGATCGCTTCCAAAGGACAAGGTGTCAACCACGTACCGAAAAG GTACAGTTTGGCGGTGGGGCCTCCAAAGAAAGACCCAAAAGTGAAAGGTGTCCAGTCGGCAGCTGTGCAA GCTTTCCTTAGGCGGAAAGAAGAGGAGCTTAGACAAAAAGCCTTGGAAGAGAAAAAAAGGAAAGAGGAAC TAGTGAAAAAGCGAATTGAGCTCAAACATGACAAAAAAGCAAGAGCCATGGCCAAGAGGACAAAGGACAA TTTTCATGGTTACGATGGGATTCCTGTTGAGGAAAAGACAAAGAAGAAGCAGCTGGTGGAGAGCCACCTG AACCAGGGAACTGACCAGGAGTATGATGTGGAGGAGGAAGACTTCATAGACTACAATCAGGCTGAGCTGG ACCAGGACTATGAGGAGGAGCAAGAGCCACCCAAGGCTGAAAGCAAACCAAAGGCCCCCCTTAAAAGTGC CCCGTCACCCATGAACTTCACTGACTTACTGAGGCTAGCTGAGAAGAAGCAGTTTGAACCAGTGGAGATC AAGGTAGTGAAGAAAGCAGAAGACCGGCCCCTGACTGCAGAGGAACTGAGGGAGCGAGAGTTTCTTGAAC GAAAGCATAGGAAAAAGAAACCTGAACCCGATGCCAAACTACCTCCACCCGTGTTGAAAAAGGCGCCCTC TCATAAAGACATCATGGGTACAAAGCCCAGCAGAGGTGCTGGGGACAGGCAGCTTGCTTCCAAAGGATTG CCCTTTCCTCAAGCTGAGAAGAAATTCAGACCCAGCACGGCCAGTGAGAAACAAGCAGCTTTGTCTTCAC CCAAATCCCTGCCAGGCGAGAGGACCAAGGTAGGGTCTGGCAGTAGCACCCAGCCCTCACTTCGGGAGGG TCACAACAGGCCTGTCTTCAATGGGGCTGGAAAGCCCCGCCCCAGCACCTGTTCACCAAGTGTCCCAAAG ACTCCTGCTAGCGGGACTCAGAAATCTGCTTCTGAGCACAAAGCCAAAAAACCTCTTCCTTCTCATCCGA GCCATTCCAAGCCTGGACCCACGGTTCTCTCACACAACAAATCTAAGAGTCCAGGTGTCAGACAACCAGG CAGCAACTCTGGCTCTGCTCCTGGGCAGCCTAACCCAGGGACAGCTCGACCCACACTTAGTTCTGGCCCT GTGCCCAGGCGCCAGAACGGCAGCTCCAGCTCAGGACCTGAGCAGTCAGCCGGTGGGATCAGAAAGCTGG CCAGCAATTCACATCTCTCCGGACGAACACTCAACGGCACAAATGGTCCTGGACGACCTGCAAGCAGCTC AAGTGGCCCTGGGCGACCCATCAGTGGATCTGCTGGTTCTGGGAGACCTGTGGGCAGTTCTGGAGGCCCC GGGCAGCCTGTAAACAATCCACATGACCTTCGACGACCAATGAACAGTCTTAGCTCTCCTGGGCGGGCAG TCAGTGGCCCTGGGAGATCCATAAGTGGTTCCATTCCAGCTGGACGAACTGTCAATTCAGGCCCTGGAAG ACCAGTGAGCAGCCTAGGCCCTGGACGAGCAGTTAGTAACCCAGGCCTCCCCACAAAGCCCAGATGTACT GTTGTCTCAGAAACAATTTCTTCAAAGAATATTATTAGTCGGTCAAGTAATGGACAGATAAATGGAATGA AACCTCTCCTCTCTGGCTACAGATCTGCTCAAGGTCCTCAAAGGCTTCCCTTCCCTACTGGCTACAAAAG GCCTCGAGAGTATGAAGAAGATGATGATGATGAATATGACTCGGAAATGGATGATTTCATCGAAGATGAA GGAGAACCTCAGGAAGAAATATCCAAGCACATTCGAGAAATCTTTGGCTATGACCGAAAAAAATATAAAG ATGAAAGTGACTATGCCTTACGTTACATGGAGAGCAGTTGGAAGGAGCAGCAGAAGGAGGAAGCCAAGAG TTTAAGACTTGGTATGCAAGAGGACTTAGAGGAAATGAGGCGTGAAGAGGAGGAACTGAAACGTCGAAAA GCCAAGAAGCTGAAGCGACACTAGCTGCTGCTACTGTAGATTCTGGAAGGGACACTGCAAGGATCACCTG CCTTCAGACTGAGCAAGCCTCTTCCCGGATTAGATTCACACTTGGGCCCGCAAGAGGAAGGAATACATAC TGTCGTTTGCAGGCTATCATTTGAGTGTGCAGTGTTTTTAAATAGCCCAGGGTGGAACCTATAATGGAGA TGATACGATGCTGGCCATGTGCTCTGTGAAGTTAGCCATTCACGGGTGCCGGCCTTGATCCTGATGAGCT ATGCACGCTAATGTGGGACCACTCACCAGGCAGCTGATCATTGTTCTTTTGCTACTTCAAAAGGAGAAAA AGATTGATGTGTGAGTAGGGTACCACCCAGATCGCAGTCTGACTTCCTTAGGAAGGGCCTGGCCCTTCTG TGAATGCTGCCAGCAATAAAGTGACCTGTCCTAGTCTATGTTTGAATTGAAAACCCAGGAAAGAGTAGGC TTTATAGATATTATATTAGTCTCATCCTTGTGACTGACACACAAAATAGAATGAATTATATTTAGCTTAT TTGTGGGTTGGATGCTAAGCATTCTTCATTGATCTTAGGTAATAAAACTTTTAAGTTAAAATACAAATTT TGCCAGAAGAAGAATGTTCTTTTCCAGTTTTCCTTCCAAGCTTTCTGTTACGTTGATTTTACATGAAGTA GATGAGAGGGCCCATACTATTGGTGATTGATTGTTGTTTGTTTGTTTCATTCCTTTTTATAGTAAAGAAA GATGGTTATTCAGATATTAGATATTTGAAAGGGTAGTTTAAAGATTAGCAAGCAACATGTATTTTGGCCC CTTCTGCTTTATTCTCCCCCATTACAATGCTATTTGGGACCCTAAGGTGCCCCTGTACCTCTTGATGTTA ATAGTTAACATTTCATTTTTAGCTTAAATTCCTTAGAAAATGTTAATAACCAAAAAGCCAAATGTTTAGT GTATCAGGAATACCACCTTCTCTTTGTCCGCGTTGCACTTTTATTTCTAACTATGCACTGTGAAGAGAAT GTTTCCTGTTGGTGTGAAACCTTCTTACGTTCTATCCTGCTTAAAGCCAGACCTACAGACAGACTTAATG CCATTTCAACCTGGAGCCCATCCAGGCTGATGTATGGTCTGGCTGGAAGGAAGCCATGTCTCAAGGACAG TCAGCAACAGAAAATGAAATAACATGCTAGAGACTGGAGTGTGGAGGTTTGGGAGCAGCCAGAATTTGGA TTGTGGGATGATCCACAATGTATCTGGCTTTCTTAAACTGTTAAGCCATGAGGGGTGGGAAGCCAGAGAG GCTATCCTACAAAGAATTCTTACTTTTCAACAGGTCCAGACTTTATGCATTTGTCCATGATGTCTTCATT CTTAAGCTTGAGTATTATGGGTAATCATGTTTTGCCTTCCTTTGTGGGACCATTTGACCCAATCCTTAAA ACAAACTCGTTTTGAATTGAGTTTCAAACTTTGAGAAAGTGCAAGGATTTAATTTTAAAGGGTATAGAAC GCTTCTAACTCTCCATGTAGGTCTTTATACACTGGTAGCAGAAAGTCTATTATTAACTTCCCTTTCCCTA GCCCACCGTTAATATAAAGGGAGGAATATTCCACTGGATAAGTATGTTTGGAACATTGTTATTGCTATCA CTTGTGTGCTTCCCCGCCTCTGTGATTTTCATATTCATACTCTGGAATCTGTAATTGCGGTCAAAGTTAC TGGTGGCTGAAAGTTTACTGTACTCAATTTCAGGGTGCAGCTGTTTGCACAGAAAGGCGCCATTGTGCTA GATTAAAGTTTCAGAACTTGGTAGAGTGAGGTTGTAATCTCTCATGTATGAATGCATTTCTGTAACAGGC ATGCATCAACTTTTACATAAGTACTCTTGTGTGGGCCATTAAAAAGACACAGGAAAGATTGTAAGTCCAA ATCATGTCTTCCATTTTGCTCTTCTGTATTTCCTGACCACTTAGAAAAAGTTCACAAGCCTTTGAAGAAA CAGCAGCAGGCTGTTATTGTTTTCAAGGTTTACAAATTAAGTGCTTGATTTTTAATGTATTCTCTCTATC TACTGTTTTGATTTTCAGATTTTGACTCAATATTTGAAGATGTGCCTTTTATTGGGTGATGTTAATTCTC GAATGTAGTAATGTTGTTTCACAATTGTAATGTTACATGTTCACGGGTGTGACATTCTCTAACAAATTAT TCGATAAGAAAGTCATTTTCATGGAACTCATGGCTCTGATTGCACAGTCCCTTGCTAAAGCAAAGGGCAC TCTTTTAAGGCTACTTCAGAACTGGTCTTTGTATTTTCTCTAAGCGATGGATGTAGTCATGTCCATTTAG AGGCAGGGTACCACATGTCACAGGCAGCCCTGAGTGTGTGCCATGAGAAAGTTCAAAGACTGTGACAAAG TCTACAGGAACTCAAAATTTCATTTTTGTAATGGTAACCCCCAAGATTTTCTCCAAGAAATAAATTATTT TGTATTTTATAACTTAAAAGCAGCTAGTAGTACATTTCTGAGATACAGCAGGAGACCAAAACATTTTTCG AAAGAGAATAAATATATAAAGAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834544 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834544 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12629 same embryo
	EMAGE:12625 same embryo
	EMAGE:12627 same embryo
	EMAGE:12628 same embryo
	EMAGE:12630 same embryo
	EurExpress:euxassay_011306 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source